5-FITC
- SKU
- F0011
Category: Fluorescent Detection, Fluorescent Detection
- Synonyms
- Fluorescein 5-isothiocyanate
- 3326-32-7
- CAS-Number
- C21H11NO5S
- Molecular Formula
- 389.38
- Molecular Weight
Specifications
- Purity
- ≥95% (HPLC)
- Appearance
- Yellow to orange powder
- Identity
- 1H-NMR
Properties
- Solvents
- aqueous buffers (pH ≥ 6)
- Melting Point
- >360 °C
- Fluorescence
- λex 492 nm, λem 518 nm in 0,1 M phosphate pH 8.0
Downloads
- Safety Data Sheet
- CDX F0011 MSDS.pdf
- Shipping
- AMBIENT
- Short Term Storage
- +4°C
- Long Term Storage
- -20°C
- Handling Advice
- Protect from light and moisture.
- Use / Stability
- Stable for at least 2 years after receipt when stored at -20°C.
- Hazard statements
- H317, H334
- Precautionary statements
- P261, P280, P342 + P311
- GHS Symbol
- GHS08
- Signal word
- Danger
- Transportation
- Not dangerous goods
- Description
- Fluorescent marker for biochemical applications. Reacts under mild conditions with primary amines. Used in modification of actin at lys-61, labelling of FAB and the modification of thiol groups.
- Smiles
- OC1=CC2=C(C=C1)C1(OC(=O)C3=C1C=CC(=C3)N=C=S)C1=C(O2)C=C(O)C=C1
- InChi Key
- MHMNJMPURVTYEJ-UHFFFAOYSA-N
- References
- (1) D-W. Han et al.: Bioorg.& Med. Chem. 16(22), 9652 (2008) , (2) L. Miller et al.: Eur. J. Biochem. 174, 23 (1998) , (3) G.P. Der-Balian et al.: Anal. Biochem. 173, 59 (1988) , (4) K. Muramoto et al.: Anal. Biochem. 141, 446 (1984) , (5) A.F. Wilderspin et al.: Anal. Biochem. 132, 449 (1983)
- InChi
- InChI=1S/C21H11NO5S/c23-12-2-5-16-18(8-12)26-19-9-13(24)3-6-17(19)21(16)15-4-1-11(22-10-28)7-14(15)20(25)27-21/h1-9,23-24H
Fluorescent marker for biochemical applications. Reacts under mild conditions with primary amines. Used in modification of actin at lys-61, labelling of FAB and the modification of thiol groups.