Specifications
- Purity
- ≥90% (NMR)
- Appearance
- White to off-white powder
- Identity
- 1H-NMR
Properties
- Solvents
- water (10 mg/ml), DMSO, DMF
Downloads
- Safety Data Sheet
- CDX B0310 MSDS.pdf
Sulfo-NHS-LC-Biotin is an amino-reactive biotinylation reagent with a long spacer arm. The 6-aminocaproic acid spacer arm provides distance between the biotin and the target ligand which helps alleviate steric hindrance, in addition it forms permanent amide bonds. The sodium sulfonate groups render this compound water-soluble and prevent it from crossing the lipid bi-layer of the cell membrane, making it a tool to label cell surface proteins. In addition, Sulfo-NHS-LC-Biotin is used to biotinylate antibodies, proteins, peptides, or amino acids by reacting with primary amines as found in the N-terminus and Lysine side chains. Optimal reaction conditions are at a pH of 7-9. Avoid amine-containing buffers, which may compete in the acylation reaction.
Synonyms | EZ-Link Sulfo-NHS-LC-Biotin, Sulfosuccinimidyl 6-(biotinamido)hexanoate, Biotinamidohexanoic acid 3-sulfo-N-hydroxysuccinimide ester sodium salt |
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Purity | ≥90% (NMR) |
Appearance | White to off-white powder |
CAS-Number | 191671-46-2 |
Molecular Formula | C20H29N4NaO9S2 |
Molecular Weight | 556.59 |
Identity | 1H-NMR |
Solvents | water (10 mg/ml), DMSO, DMF |
InChi Key | JJGWLCLUQNFDIS-GTSONSFRSA-M |
Shipping | AMBIENT |
Short Term Storage | +4°C |
Long Term Storage | -20°C |
Handling Advice | Protect from light and moisture. |
Use / Stability | Stable for at least 2 years after receipt when stored at -20°C. |
Hazard statements | H360D |
Precautionary statements | P201-P308 + P313 |
GHS Symbol | GHS08 |
Transportation | Not dangerous goods |
References | (1) G.T. Hermanson, Bioconjugate Techn. 2nd p506-545 (2008) |
Quantity | 10 mg, 50 mg, Bulk |