6-FAM N-succinimidyl ester
- SKU
- C0017
Category: Fluorescent Detection, Fluorescent Detection
- Synonyms
- 6-Carboxyfluorescein NHS ester , 6-CFSE , 6-FAM, SE
- 92557-81-8
- CAS-Number
- C25H15NO9
- Molecular Formula
- 473.39
- Molecular Weight
Specifications
- Purity
- ≥98% (HPLC)
- Appearance
- Yellow to orange powder
- Identity
- 1H-NMR
Properties
- Solvents
- DMSO, dimethylformamide
- Melting Point
- 337.43 °C (Predicted)
- Boiling Point
- 788.09 °C at 760 mmHg (Predicted)
- Refractive Index
- n20/D 1.79 (Predicted)
- Density
- 1.74 g/cm3 (Predicted)
- Fluorescence
- λex 492 nm , λem 517 nm (pH 8.0)
- Shipping
- AMBIENT
- Short Term Storage
- +4°C
- Long Term Storage
- -20°C
- Handling Advice
- Protect from light and moisture.
- Use / Stability
- Stable for at least 2 years after receipt when stored at -20°C.
- Transportation
- Not dangerous goods
- Description
- Popular green fluorescent cell-permeable dye. Covalently couples via its succinimidyl group to intracellular molecules. The amine-reactive succinimidyl ester of 6-FAM acid is a common fluorescent derivatization reagent for covalently labeling peptides, proteins and nucleotides. Due to this covalent coupling reaction it can be retained within cells for extremely long periods and due to this stable linkage, once incorporated within cells the dye is not transferred to adjacent cells. Yields carboxyamides which are more resistant to hydrolysis than fluorescein conjugates prepared with FITC. Better retained in cells than fluorescein. Spectral data: ?ex 496 nm| ?em 516 nm (Buffer pH 9.0).
- Smiles
- OC1=CC=C2C(OC(C=C(O)C=C3)=C3C24OC(C5=C4C=C(C(ON6C(CCC6=O)=O)=O)C=C5)=O)=C1
- InChi Key
- VDABVNMGKGUPEY-UHFFFAOYSA-N
- References
- (1) P. Breeuwer, et al.: Appl. Environ. Microbiol. 62, 178 (1996) , (2) M. Adamczyk, et al.: Bioconjugate Chem. 8, 253 (1997) , (3) T.S. Seo, et al.: J. Org. Chem. 68, 609 (2003) , (4) K. Tanaka, et al.: JACS 129, 5612 (2007) , (5) G.V. Los, et al.: ACS Chem. Biol. 3, 373 (2008) , (6) S. Mizukami, et al.: JACS 134, 1623 (2012)
- InChi
- InChI=1S/C25H15NO9/c27-13-2-5-16-19(10-13)33-20-11-14(28)3-6-17(20)25(16)18-9-12(1-4-15(18)24(32)34-25)23(31)35-26-21(29)7-8-22(26)30/h1-6,9-11,27-28H,7-8H2
Popular green fluorescent cell-permeable dye. Covalently couples via its succinimidyl group to intracellular molecules. The amine-reactive succinimidyl ester of 6-FAM acid is a common fluorescent derivatization reagent for covalently labeling peptides, proteins and nucleotides. Due to this covalent coupling reaction it can be retained within cells for extremely long periods and due to this stable linkage, once incorporated within cells the dye is not transferred to adjacent cells. Yields carboxyamides which are more resistant to hydrolysis than fluorescein conjugates prepared with FITC. Better retained in cells than fluorescein. Spectral data: ?ex 496 nm| ?em 516 nm (Buffer pH 9.0).