Fluorescamine
- SKU
- F0003
Category: Fluorescent Detection, Fluorescent Detection
- Synonyms
- 1'(3'H)-isobenzofuran]-3,3'-dione, 4-phenyl-spiro[furan-2(3H) , 4-Phenylspiro[furan-2(3H),1'-phthalan]-3,3'-dione , Fluram
- 38183-12-9
- CAS-Number
- C17H10O4
- Molecular Formula
- 278.26
- Molecular Weight
Specifications
- Purity
- ≥98% (TLC)
- Appearance
- Off-white to yellow powder
- Identity
- 1H-NMR
Properties
- Solvents
- acetone (50 mg/ml), water (partly), ethanol, chloroform, DMSO, acetonitrile
- Melting Point
- 153-157 °C (lit.)
- UV / Vis
- λex 234 nm, λex 390 nm, λem 475 nm in 0.5 M borate pH 8.5 (after derivatization with L-leuc
- Shipping
- AMBIENT
- Short Term Storage
- RT
- Long Term Storage
- RT
- Handling Advice
- Protect from light and moisture.
- Use / Stability
- Stable for at least 2 years after receipt when stored at RT.
- Transportation
- Not dangerous goods
- Description
- A non-fluorescent reagent that reacts readily under mild conditions with primary amino groups in amino acids and peptides to form stable and highly fluorescent derivatives. Low background due to hydrolysis. Useful in fluorometric assays of amino acids, protein, and proteolytic enzymes. Effectively blocks newly generated amino termini in protein sequence analysis by the Beckman automated sequencer. Spectral data: Exc. 390nm/Em. 475 nm in 0.5 M borate buffer, pH 8.5, after derivatization with L-leucine.
- Smiles
- O=C1C(C2=CC=CC=C2)=COC31OC(C4=CC=CC=C43)=O
- InChi Key
- ZFKJVJIDPQDDFY-UHFFFAOYSA-N
- References
- (1) S. Udenfriend, et al.: Science 178, 871 (1972) , (2) M. Weigele, et al.: JACS 94, 5927 (1972) , (3) S. Stein, et al.: Arch. Biochem. Biophys. 155, 202 (1973) , (4) P. Bohlen, et al.: Arch. Biochem. Biophys. 163, 390 (1974) , (5) C.Y. Lai, et al.: Meth. Enzymol. 47, 236 (1977) , (6) K. Sogowa & K.J. Takahaski: Biochem. 83, 1783 (1978) , (7) A.S. Bhown, et al.: Anal. Biochem. 112, 158 (1981)
- InChi
- InChI=1S/C17H10O4/c18-15-13(11-6-2-1-3-7-11)10-20-17(15)14-9-5-4-8-12(14)16(19)21-17/h1-10H
A non-fluorescent reagent that reacts readily under mild conditions with primary amino groups in amino acids and peptides to form stable and highly fluorescent derivatives. Low background due to hydrolysis. Useful in fluorometric assays of amino acids, protein, and proteolytic enzymes. Effectively blocks newly generated amino termini in protein sequence analysis by the Beckman automated sequencer. Spectral data: Exc. 390nm/Em. 475 nm in 0.5 M borate buffer, pH 8.5, after derivatization with L-leucine.