Specifications
- Purity
- for microscopy
- Appearance
- Red to very dark red liquid
- Identity
- UV/Vis
- UV/Vis
- 525 - 535 nm
Properties
- Solvents
- ethanol
- Refractive Index
- n20/D 1.338 - 1.340
- Density
- 0.986 g/ml
Downloads
- Safety Data Sheet
- CDX G0061 MSDS.pdf
Category: Non Fluorescent Detection & Chromogene Detection
Gram Safranin Solution is a reagent recommended for use in qualitative procedures to differentiate gram-negative from gram-positive organisms. The Gram staining allows a fast differentiation of bacteria in Gram-positive and Gram-negative. The mureine structure of the bacteria walls is the basis of the color affinity. Bacteria will be stained with Gram’s crystal violet solution – an aniline dye – in the first step. After the treatment with iod solution, a dye-iod complex will form. During the decolorizing step, this complex stays in the multilayer mureine structures of the Gram-positive bacteria and they will appear blue. Gram-negative bacteria have a monolayer mureine structure only, the dye-iod complex does not stay bound to the cellwall, they will be decolorized. Gram-negative bacteria will be counterstained by safranin solution and appear orange.
Synonyms | Safranin O Solution, Safarin T Solution |
---|---|
Purity | for microscopy |
Appearance | Red to very dark red liquid |
CAS-Number | 477-73-6 (comp.) |
Molecular Formula | C20H19ClN4 |
Molecular Weight | 350.84 |
Identity | UV/Vis |
Solvents | ethanol |
Refractive Index | n20/D 1.338 – 1.340 |
Density | 0.986 g/ml |
Smiles | CC1=C(N)C=C([N+](C2=CC=CC=C2)=C(C=C(N)C(C)=C3)C3=N4)C4=C1.[Cl-] |
InChi Key | OARRHUQTFTUEOS-UHFFFAOYSA-N |
Shipping | AMBIENT |
Short Term Storage | RT |
Long Term Storage | RT |
Handling Advice | Protect from light and moisture. |
Use / Stability | This product should not be used if colour has changed or if there are |
Hazard statements | H226 |
GHS Symbol | GHS02 |
Signal word | Warning |
Transportation | Packing Group III |
References | (1) E. Adams, Stain Technol. 50, 227 (1975), (2) R. Coico, et al., Curr. Protoc. Microbiol. Appendix 3-3C (2005) |
Quantity | 250 ml, 2.5 l, Bulk |
cdx-UV/Vis | 525 – 535 nm |