Specifications
- Purity
- ≥99% (HPLC)
- Appearance
- White to light yellow powder
- Identity
- 1H-NMR
Properties
- Solvents
- DMSO, water (insoluble)
- Melting Point
- 168-171 °C (lit.)
Category: Fluorescent Detection, Fluorescent Detection
3,3?,5,5?-Tetramethylbenzidine (TMB) chromogenic peroxidase sensitive dye. TMB is a noncarcinogenic substitute (Ames test negative) for benzidine suitable as peroxidase substrate for use in ELISA procedures. The substrate produces a soluble end product that is pale blue in color and can be read spectrophotometrically at 370 or 620-650nm. The TMB reaction may be stopped with 2M H2SO4 (resulting in a yellow color), and read at 450nm. A sensitive and specific reagent for the detection of blood, assay of haemoglobin and assay of horseradish peroxidase (HRP).
Synonyms | 3,3',5,5'-Tetramethylbenzidine, BM blue, Sure Blue TMB, TMB Blotting Plus, TMB substrate, TMBZ |
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Purity | ≥99% (HPLC) |
Appearance | White to light yellow powder |
CAS-Number | 54827-17-7 |
Molecular Formula | C16H20N2 |
Molecular Weight | 240.34 |
Identity | 1H-NMR |
Solvents | DMSO, water (insoluble) |
Melting Point | 168-171 °C (lit.) |
Smiles | CC1=CC(=CC(=C1N)C)C2=CC(=C(C(=C2)C)N)C |
InChi Key | UAIUNKRWKOVEES-UHFFFAOYSA-N |
Shipping | AMBIENT |
Short Term Storage | +4°C |
Long Term Storage | +4°C |
Handling Advice | Protect from light and moisture. |
Use / Stability | Stable for at least 2 years after receipt when stored at +4°C. |
Transportation | Not dangerous goods |
References | (1) R.C. Lijana & M.C. Williams, J. Lab. Clin. Med. 94, 266 (1979), (2) E.S. Bos, et al., J. Immunoassay 2, 187 (1981), (3) D.B. Rye, et al., J. Histochem. Cytochem. 32, 1145 (1984), (4) A.K. Goka & M.J. Farthing, J. Immunoassay 8, 29 (1987), (5) J.L. McKimm-Breschkin, J. Immunol. Methods 135, 277 (1990), (6) A. Frey, et al., J. Immunol. Methods 233, 47 (2000), (7) R. Georgescu, Methods Mol. Biol. 230, 171 (2003), (8) L. Zhang & J. Du, Spectrochim. Acta A Mol. Biomol. Spectrosc. 158, 24 (2016), (9) C. Lei, et al., Talanta 175, 457 (2017) |
Quantity | 1 g, 5 g, 25 g, Bulk |