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2,6-Dichloroindophenol sodium salt hydrate

SKU: D1204

Category: Non Fluorescent Detection & Chromogene Detection

Synonyms: 2,6-Dichloro-N-(4-hydroxyphenyl)-1,4-benzoquinoneimine sodium salt, 2,6-Dichlorobenzenone-indophenol sodium salt, 2,6-Dichlorophenolindophenol sodium salt hydrate, DCIP, DPIP, Tillmans' reagent; 1266615-56-8
CAS-Number: C₁₂H₆Cl₂NNaO₂ · xH₂O
Molecular Formula: 290.08 (anhydrous basis)
Molecular Weight

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Product Data
Handling
Safety Information
Details

Specifications

Purity: ≥95 (NMR)
Appearance: Blue to dark-blue powder
Identity: 1H-NMR

Properties

Solvents: water (10mg/ml)

Shipping: AMBIENT
Short Term Storage: RT
Long Term Storage: RT
Handling Advice: Protect from light and moisture.
Use / Stability: Stable for at least 2 years after receipt when stored at RT.

Transportation: Not dangerous goods

Description: 2,6-Dichloroindophenol sodium salt hydrate (DCIP) is a blue redox dye. It is an oxidizing agent and can be used as a redox indicator. The color change occurs through a redox reaction. In the oxidized quinone imine form, dichlorophenol-indophenol is blue or red, in the reduced aminodiphenol form it is colorless. DCIP is suitable for the quantitative determination of ascorbic acid (vitamin C). The ascorbic acid is oxidized and in return the colored dichlorophenol-indophenol solution is reduced and thus decolorized. The consumption of the reagent in redox reactions can be determined photometrically. It has been used as a redox dye to investigate the efficiency of gold nanoparticles (Au-NP) for the enzymatic activity of glucose oxidase and to compose UVB specific dosimeter and to measure the rate of photosynthesis. DCIP has been used as substrate in NQO1 assays to investigate the liver cytosolic NQO1 activity spectrophotometrically and in mitochondrial succinate CoQ oxidoreductase (complex II) activity assay. In thin layer chromatography the compound serves as a reagent for organic acids and reducing compounds.
Smiles: [O-]C1=CC=C(/N=C2C=C(C(C(Cl)=C\2)=O)Cl)C=C1.O.[Na+]
InChi Key: XHSOLXWCGCVQHE-UHFFFAOYSA-M
References: (1) H. Yao, et al.; Clin. Chem. 41, 591 (1995) , (2) A. Ramanaviciene, et al.; Sens. Actuat. B Chem. 137, 483 (2009) , (3) A. Mills & P. Grosshans; Analyst. 134, 845 (2009) , (4) J. Qiu, et al.; Chemosphere 77, 129 (2009) , (5) G.P. Rizzi, et al.; J. Agric. Food Chem. 58, 9739 (2010) , (6) C.A. Vieira CA, et al.; Biosci. 26, 296 (2010) , (7) S. Han, et al.; J. Microbiol. Methods 90, 206 (2012) , (8) M. Pohanka & L. Drtinova; Talanta 106, 281 (2013) , (9) S.H. Chiu & P.L. Urban; Analyst 140, 5145 (2015) , (10) P. Bollella, et al.; Anal. Bioanal. Chem. 410, 3253 (2018)
InChi: InChI=1S/C12H7Cl2NO2.Na.H2O/c13-10-5-8(6-11(14)12(10)17)15-7-1-3-9(16)4-2-7;;/h1-6,16H;;1H2/q;+1;/p-1

2,6-Dichloroindophenol sodium salt hydrate (DCIP) is a blue redox dye. It is an oxidizing agent and can be used as a redox indicator. The color change occurs through a redox reaction. In the oxidized quinone imine form, dichlorophenol-indophenol is blue or red, in the reduced aminodiphenol form it is colorless. DCIP is suitable for the quantitative determination of ascorbic acid (vitamin C). The ascorbic acid is oxidized and in return the colored dichlorophenol-indophenol solution is reduced and thus decolorized. The consumption of the reagent in redox reactions can be determined photometrically. It has been used as a redox dye to investigate the efficiency of gold nanoparticles (Au-NP) for the enzymatic activity of glucose oxidase and to compose UVB specific dosimeter and to measure the rate of photosynthesis. DCIP has been used as substrate in NQO1 assays to investigate the liver cytosolic NQO1 activity spectrophotometrically and in mitochondrial succinate CoQ oxidoreductase (complex II) activity assay. In thin layer chromatography the compound serves as a reagent for organic acids and reducing compounds.

SKU: D1204 Category: Non Fluorescent Detection & Chromogene Detection

Additional information

Additional information

Synonyms	2,6-Dichloro-N-(4-hydroxyphenyl)-1,4-benzoquinoneimine sodium salt, 2,6-Dichlorobenzenone-indophenol sodium salt, 2,6-Dichlorophenolindophenol sodium salt hydrate, DCIP, DPIP, Tillmans' reagent
Purity	≥95 (NMR)
Appearance	Blue to dark-blue powder
CAS-Number	1266615-56-8
Molecular Formula	C12H6Cl2NNaO2 · xH2O
Molecular Weight	290.08 (anhydrous basis)
Identity	1H-NMR
Solvents	water (10mg/ml)
Smiles	[O-]C1=CC=C(/N=C2C=C(C(C(Cl)=C\2)=O)Cl)C=C1.O.[Na+]
InChi Key	XHSOLXWCGCVQHE-UHFFFAOYSA-M
Shipping	AMBIENT
Short Term Storage	RT
Long Term Storage	RT
Handling Advice	Protect from light and moisture.
Use / Stability	Stable for at least 2 years after receipt when stored at RT.
Transportation	Not dangerous goods
References	(1) H. Yao, et al.; Clin. Chem. 41, 591 (1995), (2) A. Ramanaviciene, et al.; Sens. Actuat. B Chem. 137, 483 (2009), (3) A. Mills & P. Grosshans; Analyst. 134, 845 (2009), (4) J. Qiu, et al.; Chemosphere 77, 129 (2009), (5) G.P. Rizzi, et al.; J. Agric. Food Chem. 58, 9739 (2010), (6) C.A. Vieira CA, et al.; Biosci. 26, 296 (2010), (7) S. Han, et al.; J. Microbiol. Methods 90, 206 (2012), (8) M. Pohanka & L. Drtinova; Talanta 106, 281 (2013), (9) S.H. Chiu & P.L. Urban; Analyst 140, 5145 (2015), (10) P. Bollella, et al.; Anal. Bioanal. Chem. 410, 3253 (2018)
InChi	InChI=1S/C12H7Cl2NO2.Na.H2O/c13-10-5-8(6-11(14)12(10)17)15-7-1-3-9(16)4-2-7;;/h1-6,16H;;1H2/q;+1;/p-1
Quantity	5 g, 25 g, Bulk