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Available from stock

2′,7′-Dichlorofluorescein

SKU
D0004

Category:

Synonyms
Dichlorofluorescein , DCF
76-54-0
CAS-Number
C20H10Cl2O5
Molecular Formula
401.2
Molecular Weight

Specifications

Purity
≥90% (T)
Identity
1H-NMR
Appearance
Beige powder

Properties

Fluorescence
λex 504 nm , λem 529 nm in 0.1 M Tris pH 8.0
Melting Point
280 °C (dec.)(lit.)
Solvents
ethanol (25 mg/ml), water (01mg/ml), DMSO

Downloads

Safety Data Sheet
CDX D0004 MSDS.pdf
Long Term Storage
+4°C
Use / Stability
Stable for at least 2 years after receipt when stored at +4°C.
Handling Advice
Protect from light and moisture.
Short Term Storage
+4°C
Shipping
AMBIENT
Transportation
Not dangerous goods
Signal word
Warning
GHS Symbol
GHS07
Precautionary statements
P261-P305 + P351 + P338
Hazard statements
H315-H319-H335
Description
Fluorescent dye. Spectral data: Extinction 504nm/Emmission 529nm, in 0.1 M Tris pH 8.0. Used for the determination of carbohydrates by fluorescence densitometry after TLC, in the determination of H2O2 by chemiluminescence, and as a fluorescent additive used in indirect fluorimetric detection in OTC-HPLC. Used as a component/substrate of the 2?,7?-dichlorofluorescein diacetate (DCFH-DA) assay to quantitate reactive oxygen species (ROS).
InChi
InChI=1S/C20H10Cl2O5/c21-13-5-11-17(7-15(13)23)26-18-8-16(24)14(22)6-12(18)20(11)10-4-2-1-3-9(10)19(25)27-20/h1-8,23-24H
References
(1) M.L. Baumann & A.L. Hatmaker , Clin. Chem. 7, 356 (1961) , (2) G. Gübitz, et al.: J. Chromatogr. 117, 337, (1976) , (3) H. Watanabe, et al.: Anal. Sci. 2, 461, (1986) , (4) W.D. Pfeffer & E.S. Yeung , J. Chromatogr. 506, 401, (1990) , (5) H. Zhu, et al.: Arch. Toxicol. 68, 582 (1994)
InChi Key
VFNKZQNIXUFLBC-UHFFFAOYSA-N
Smiles
O=C(C1=C2C=CC=C1)OC32C4=C(C=C(O)C(Cl)=C4)OC5=CC(O)=C(Cl)C=C53

Fluorescent dye. Spectral data: Extinction 504nm/Emmission 529nm, in 0.1 M Tris pH 8.0. Used for the determination of carbohydrates by fluorescence densitometry after TLC, in the determination of H2O2 by chemiluminescence, and as a fluorescent additive used in indirect fluorimetric detection in OTC-HPLC. Used as a component/substrate of the 2?,7?-dichlorofluorescein diacetate (DCFH-DA) assay to quantitate reactive oxygen species (ROS).

Artikelnummer: D0004 Kategorie:

Zusätzliche Information

Synonyms

Dichlorofluorescein, DCF
Purity

≥90% (T)
Appearance

Beige powder
CAS-Number

76-54-0
Molecular Formula

C20H10Cl2O5
Molecular Weight

401.2
Identity

1H-NMR
Solvents

ethanol (25 mg/ml), water (01mg/ml), DMSO
Melting Point

280 °C (dec.)(lit.)
Fluorescence

λex 504 nm, λem 529 nm in 0.1 M Tris pH 8.0
Smiles

O=C(C1=C2C=CC=C1)OC32C4=C(C=C(O)C(Cl)=C4)OC5=CC(O)=C(Cl)C=C53
InChi Key

VFNKZQNIXUFLBC-UHFFFAOYSA-N
Shipping

AMBIENT
Short Term Storage

+4°C
Long Term Storage

+4°C
Handling Advice

Protect from light and moisture.
Use / Stability

Stable for at least 2 years after receipt when stored at +4°C.
Hazard statements

H315-H319-H335
Precautionary statements

P261-P305 + P351 + P338
GHS Symbol

GHS07
Signal word

Warning
Transportation

Not dangerous goods
References

(1) M.L. Baumann & A.L. Hatmaker, Clin. Chem. 7, 356 (1961), (2) G. Gübitz, et al.: J. Chromatogr. 117, 337, (1976), (3) H. Watanabe, et al.: Anal. Sci. 2, 461, (1986), (4) W.D. Pfeffer & E.S. Yeung, J. Chromatogr. 506, 401, (1990), (5) H. Zhu, et al.: Arch. Toxicol. 68, 582 (1994)
InChi

InChI=1S/C20H10Cl2O5/c21-13-5-11-17(7-15(13)23)26-18-8-16(24)14(22)6-12(18)20(11)10-4-2-1-3-9(10)19(25)27-20/h1-8,23-24H
Quantity

10 g, 25 g, 100 g, Bulk

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