4-Methylumbelliferyl phosphate disodium salt

SKU: M0012

Category: Fluorescent Detection

Synonyms: 4-MUP , 4-MU-phopahte , MUP , Disodium 4-methylumbelliferyl phosphate , 7-hydroxy-4-Methylcoumarin phosphate disodium salt; 22919-26-2
CAS-Number: C₁₀H₇Na₂O₆P
Molecular Formula: 300.11
Molecular Weight

Product Data
Handling
Safety Information
Details

Specifications

Purity: ≥97% (NMR)
Identity: 1H-NMR
Appearance: White to off-white powder

Properties

Fluorescence: λex 319 nm, λem 384 nm (pH 9.0)
Solvents: water (50 mg/ml)

Short Term Storage: +4°C
Use / Stability: Stable for at least 2 years after receipt when stored at -20°C.
Handling Advice: Protect from light and moisture.
Long Term Storage: -20°C
Shipping: AMBIENT

Transportation: Not dangerous goods

Description: 4-Methylumbelliferyl phosphate (4-MUP) is a fluorogenic substrate for phosphatases (mainly alkaline). It is converted to the fluorescent product 4-methylumbelliferone (4-MU), which has an emission maximum at 445-454 nm. The excitation maximum for 4-MU is pH-dependent: 330, 370, and 385 nm at pH 4.6, 7.4, and 10.4, respectively. Therefore, this phosphatase substrate is not well suited for living cell or continuous assays since 4-MU only develops maximum fluorescence at a pH value of >10. Spectral data: λex=319nm, λem=384nm (pH 9.0). λex=360nm, λem=449nm (for reaction product).
InChi: InChI=1S/C10H9O6P.2Na/c1-6-4-10(11)15-9-5-7(2-3-8(6)9)16-17(12,13)14 , /h2-5H,1H3,(H2,12,13,14) , /q , 2*+1/p-2
References: (1) D. Robinson & P. Willcox, Biochim. Biophys. Acta 191, 183 (1969) , (2) C.J. Cornish, et al., Am. J. Clin. Pathol. 53, 68 (1970) , (3) J.P. Chambers, et al., Clin. Chim. Acta 80, 67 (1977) , (4) F. Watanabe, et al., Clin. Chim. Acta 91, 273 (1979) , (5) F.A. Anthony, et al., Anal. Biochem. 155, 103 (1986) , (6) K. Soyama, et al., Forensic Sci. Int. 18, 195 (1981) , (7) H.J. Radzun, et al., Clin. Chim. Acta 123, 203 (1982) , (8) F.A. Anthony, et al., Anal. Biochem. 155, 103 (1986) , (9) K.Y. Xiao, et al., J. Tongji Med. Univ. 13, 206 (1993) , (10) K. Bednarska, et al., Mediators Inflamm. 2006, 19307 (2006) , (11) J.J. Weiland, et al., Anal. Biochem. 361, 140 (2007)
InChi Key: WUUDJQVNZPEPKN-UHFFFAOYSA-L
Smiles: CC(C1=CC=C(OP([O-])([O-])=O)C=C1O2)=CC2=O.[Na+].[Na+]

4-Methylumbelliferyl phosphate (4-MUP) is a fluorogenic substrate for phosphatases (mainly alkaline). It is converted to the fluorescent product 4-methylumbelliferone (4-MU), which has an emission maximum at 445-454 nm. The excitation maximum for 4-MU is pH-dependent: 330, 370, and 385 nm at pH 4.6, 7.4, and 10.4, respectively. Therefore, this phosphatase substrate is not well suited for living cell or continuous assays since 4-MU only develops maximum fluorescence at a pH value of >10. Spectral data: λex=319nm, λem=384nm (pH 9.0). λex=360nm, λem=449nm (for reaction product).

Artikelnummer: M0012 Kategorie: Fluorescent Detection

Zusätzliche Information

Zusätzliche Information

Synonyms	4-MUP, 4-MU-phopahte, MUP, Disodium 4-methylumbelliferyl phosphate, 7-hydroxy-4-Methylcoumarin phosphate disodium salt
Purity	≥97% (NMR)
Appearance	White to off-white powder
CAS-Number	22919-26-2
Molecular Formula	C10H7Na2O6P
Molecular Weight	300.11
Identity	1H-NMR
Solvents	water (50 mg/ml)
Fluorescence	λex 319 nm, λem 384 nm (pH 9.0)
Smiles	CC(C1=CC=C(OP([O-])([O-])=O)C=C1O2)=CC2=O.[Na+].[Na+]
InChi Key	WUUDJQVNZPEPKN-UHFFFAOYSA-L
Shipping	AMBIENT
Short Term Storage	+4°C
Long Term Storage	-20°C
Handling Advice	Protect from light and moisture.
Use / Stability	Stable for at least 2 years after receipt when stored at -20°C.
Transportation	Not dangerous goods
References	(1) D. Robinson & P. Willcox, Biochim. Biophys. Acta 191, 183 (1969), (2) C.J. Cornish, et al., Am. J. Clin. Pathol. 53, 68 (1970), (3) J.P. Chambers, et al., Clin. Chim. Acta 80, 67 (1977), (4) F. Watanabe, et al., Clin. Chim. Acta 91, 273 (1979), (5) F.A. Anthony, et al., Anal. Biochem. 155, 103 (1986), (6) K. Soyama, et al., Forensic Sci. Int. 18, 195 (1981), (7) H.J. Radzun, et al., Clin. Chim. Acta 123, 203 (1982), (8) F.A. Anthony, et al., Anal. Biochem. 155, 103 (1986), (9) K.Y. Xiao, et al., J. Tongji Med. Univ. 13, 206 (1993), (10) K. Bednarska, et al., Mediators Inflamm. 2006, 19307 (2006), (11) J.J. Weiland, et al., Anal. Biochem. 361, 140 (2007)
InChi	InChI=1S/C10H9O6P.2Na/c1-6-4-10(11)15-9-5-7(2-3-8(6)9)16-17(12,13)14, /h2-5H,1H3,(H2,12,13,14), /q, 2*+1/p-2
Quantity	250 mg, 1 g, Bulk