Specifications
- Purity
- ≥50% (UV/Vis)
- Appearance
- Dark blue solid
- Identity
- UV/Vis
- UV/Vis
- λmax 610 - 630 nm
Properties
- Solvents
- water (1 mg/ml)
- UV / Vis
- λmax 615 nm
Documentation
- Safety Data Sheet (SDS)
- CDX A0001 MSDS.pdf
Category: Non Fluorescent Detection & Chromogene Detection
Alcian blue 8GX is primarily used for demonstrating acid mucopolysaccharides, which it does quite selectively. In recent years there has been some difficulty obtaining the dye. Alcian Blue 8GX is also suitable for detection of glycoproteins on nitrocellulose and in PAGE gels.There are other dyes similar to alcian blue, although not necessarily belonging to the same class of dyes. Alcian green has gained some popularity, and alcian yellow has been suggested, especially in a method for demonstrating helicobacter. They are used for acid mucopolysaccharides in the same way as alcian blue. They have no particular advantage for demonstrating mucin. According to the Merck Index the groups marked X split off during staining.
Synonyms | Ingrain Blue 1, Alcian Blue, 75881-23-1, Alcian Blue 8GS |
---|---|
Purity | ≥50% (UV/Vis) |
Appearance | Dark blue solid |
CAS-Number | 33864-99-2 |
Molecular Formula | C56H68Cl4CuN16S4 |
Molecular Weight | 1298.87 |
Identity | UV/Vis |
Solvents | water (1 mg/ml) |
UV / Vis | λmax 615 nm |
Smiles | [Cl-].[Cl-].[Cl-].[Cl-].CN(C)C(SCC1=CC2=C(C=C1)C1=NC3=C4C=C(CSC(N(C)C)=[N+](C)C)C=CC4=C4N=C5/N=C(/N=C6N([Cu]N34)C(=N/C2=N1)C1=C6C=C(CSC(N(C)C)=[N+](C)C)C=C1)C1=C5C=C(CSC(N(C)C)=[N+](C)C)C=C1)=[N+](C)C |
InChi Key | XRWQTDIELZDCPS-UHFFFAOYSA-J |
Shipping | AMBIENT |
Short Term Storage | +4°C |
Long Term Storage | +4°C |
Handling Advice | Protect from light and moisture. |
Use / Stability | Stable for at least 2 years after receipt when stored at +4°C. |
Transportation | Not dangerous goods |
References | (1) S. Mozersky et al.: J. Am. Leather Chem. Assoc. 98(9), 337 (2003), (2) M. Hattori et al.: Bunseki Kagaku 52(4), 259 (2003), (3) P. Whiteman et al.: Biochem. J. 131(2), 343 (1973) |
InChi | InChI=1S/C56H68N16S4.4ClH.Cu/c1-65(2)53(66(3)4)73-29-33-17-21-37-41(25-33)49-59-45(37)57-46-39-23-19-35(31-75-55(69(9)10)70(11)12)27-43(39)51(61-46)64-52-44-28-36(32-76-56(71(13)14)72(15)16)20-24-40(44)48(62-52)58-47-38-22-18-34(26-42(38)50(60-47)63-49)30-74-54(67(5)6)68(7)8 |
Quantity | 5 g, 20 g, 250 g, Bulk |
cdx-UV/Vis | λmax 610 – 630 nm |