Available from stock

Cholecalciferol

SKU: C0668

Category: Steroids

Synonyms: (+)-Vitamin D3, 9,10-Secocholesta-5,7,10(19)-trien-3?-ol, Oleovitamin D3, NSC 375571, Calcirol; 67-97-0
CAS-Number: C₂₇H₄₄O
Molecular Formula: 384.64
Molecular Weight

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Product Data
Handling
Safety Information
Details

Specifications

Purity: USP41
Appearance: White to off white powder
Loss on Drying: ≤5%

Properties

Solvents: Soluble in DMSO (2mg/ml), ethanol (25mg/ml), DMF (25mg/ml) or chloroform.
Melting Point: 83-86 °C (lit.)

Shipping: AMBIENT
Short Term Storage: RT
Long Term Storage: +4°C
Handling Advice: Protect from light and moisture.
Use / Stability: Stable for at least 2 years after receipt when stored at +4°C.

Hazard statements: H301+H311-H330-H372
Precautionary statements: P280-P301+P310+P330-P302+P352+P312-P304+P340+P310-P314
GHS Symbol: GHS06+GHS08
Signal word: Danger
RIDADR: UN2811
Transportation: Packing Group II

Description: Cholecalciferol (Vitamin D₃) is a naturally occurring secosteroid and a key precursor in the vitamin D metabolic pathway. This inactive precursor to calcitriol (Vitamin D) is converted to active metabolites in vivo. Following hydroxylation in vivo, cholecalciferol is converted to biologically active vitamin D metabolites that interact with the vitamin D receptor (VDR) to regulate gene expression. Cholecalciferol is widely used in biochemical, cell culture, and metabolic research to study vitamin D signaling, calcium homeostasis, and endocrine regulation. Formulations containing vitamin D3 have been used in the treatment of osteoporosis. Cholecalciferol (vitamin D3) appears to stimulate the body's interferon type I signaling system that protects against bacteria and viruses.
Smiles: CC(C)CCC[C@@H](C)[C@@]1([H])CC[C@@]([C@]1(C)CCC/2)([H])C2=C\C=C(C[C@@H](O)CC3)/C3=C
InChi Key: _x000D_QYSXJUFSXHHAJI-YRZJJWOYSA-N
References: [1] T.C. Chen, et al.; Arch. Biochem. Biophys. 460, 213 (2007) , [2] M.F. Holick; N. Engl. J. Med. 357, 266 (2007) , [3] C.J. Rosen; N. Engl. J. Med. 364, 248 (2011) , [4] R. Wickham; J. Adv. Pract. Oncol. 3, 249 (2012) (Review) , [5] E. Zoico, et al.; Endocrinol. 155, 4178 (2014) , [6] R. Vieth; Eur. J. Clin. Nutr. 74, 1493 (2020) (Review) , [7] E. Ortiz-Prado, et al.; Front. Nutr. 12, 1579957 (2025) (Review)

Cholecalciferol (Vitamin D₃) is a naturally occurring secosteroid and a key precursor in the vitamin D metabolic pathway. This inactive precursor to calcitriol (Vitamin D) is converted to active metabolites in vivo. Following hydroxylation in vivo, cholecalciferol is converted to biologically active vitamin D metabolites that interact with the vitamin D receptor (VDR) to regulate gene expression. Cholecalciferol is widely used in biochemical, cell culture, and metabolic research to study vitamin D signaling, calcium homeostasis, and endocrine regulation. Formulations containing vitamin D3 have been used in the treatment of osteoporosis. Cholecalciferol (vitamin D3) appears to stimulate the body’s interferon type I signaling system that protects against bacteria and viruses.

SKU: C0668 Category: Steroids

Additional information

Additional information

Synonyms	(+)-Vitamin D3, 9,10-Secocholesta-5,7,10(19)-trien-3?-ol, Oleovitamin D3, NSC 375571, Calcirol
Purity	USP41
Appearance	White to off white powder
CAS-Number	67-97-0
Molecular Formula	C27H44O
Molecular Weight	384.64
Solvents	Soluble in DMSO (2mg/ml), ethanol (25mg/ml), DMF (25mg/ml) or chloroform.
Melting Point	83-86 °C (lit.)
Smiles	CC(C)CCC[C@@H](C)[C@@]1([H])CC[C@@]([C@]1(C)CCC/2)([H])C2=C\C=C(C[C@@H](O)CC3)/C3=C
InChi Key	_x000D_QYSXJUFSXHHAJI-YRZJJWOYSA-N
Shipping	AMBIENT
Short Term Storage	RT
Long Term Storage	+4°C
Handling Advice	Protect from light and moisture.
Use / Stability	Stable for at least 2 years after receipt when stored at +4°C.
Hazard statements	H301+H311-H330-H372
Precautionary statements	P280-P301+P310+P330-P302+P352+P312-P304+P340+P310-P314
GHS Symbol	GHS06+GHS08
Signal word	Danger
RIDADR	UN2811
Transportation	Packing Group II
References	[1] T.C. Chen, et al.; Arch. Biochem. Biophys. 460, 213 (2007), [2] M.F. Holick; N. Engl. J. Med. 357, 266 (2007), [3] C.J. Rosen; N. Engl. J. Med. 364, 248 (2011), [4] R. Wickham; J. Adv. Pract. Oncol. 3, 249 (2012) (Review), [5] E. Zoico, et al.; Endocrinol. 155, 4178 (2014), [6] R. Vieth; Eur. J. Clin. Nutr. 74, 1493 (2020) (Review), [7] E. Ortiz-Prado, et al.; Front. Nutr. 12, 1579957 (2025) (Review)
Quantity	1 g, 5 x 1 g, Bulk
cdx-Loss on Drying	≤5%