Specifications
- Purity
- ≥98% (NMR)
- Appearance
- Orange solid
- Identity
- 1H-NMR
Properties
- Solvents
- water
- Melting Point
- 218-220 °C
- Fluorescence
- λex 398 nm, λem 482 nm in 0.1 M phosphate pH 7.0 (after derivatization with 2-mercaptoethanol)
Available from stock
DACM
- SKU
- D0047
Categories: Fluorescent Detection, Fluorescent Detection
- Synonyms
- N-(7-Dimethylamino-4-methylcoumarin-3-yl) maleimide
- 55145-14-7
- CAS-Number
- C16H14N2O4
- Molecular Formula
- 298.29
- Molecular Weight
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
- Shipping
- AMBIENT
- Short Term Storage
- +4°C
- Long Term Storage
- -20°C
- Handling Advice
- Protect from light and moisture.
- Use / Stability
- Stable for at least 2 years after receipt when stored at -20°C.
- Hazard statements
- H315, H335
- Precautionary statements
- P261
- GHS Symbol
- GHS07
- Signal word
- Warning
- Transportation
- Not dangerous goods
- Description
- Excellent blue fluorescent thiol-reactive reagent (Ex/Em: 383/463nm), widely used for probing configurations of biomolecules such as proteins and create blue-fluorescent bioconjugates. The unreacted reagent is non-fluorescent and can also be used to quantitate free thiols. Shows superior fluorescence and water solubility properties than dansyl chloride.
- Smiles
- CN(C)C1=CC=C2C(OC(=O)C(N3C(=O)C=CC3=O)=C2C)=C1
- InChi Key
- ADEORFBTPGKHRP-UHFFFAOYSA-N
- References
- (1) L. Pollegioni et al.: Arch. of Biochem. Bioph. 343(1), 1 (1997) , (2) B. Werneburg et al.: Arch. of Biochem. Bioph. 303(2), 214 (1993) , (3) Y. Kanaoka et al.: Angew. Chem. 89, 141 (1977)
- InChi
- InChI=1S/C16H14N2O4/c1-9-11-5-4-10(17(2)3)8-12(11)22-16(21)15(9)18-13(19)6-7-14(18)20/h4-8H,1-3H3
Excellent blue fluorescent thiol-reactive reagent (Ex/Em: 383/463nm), widely used for probing configurations of biomolecules such as proteins and create blue-fluorescent bioconjugates. The unreacted reagent is non-fluorescent and can also be used to quantitate free thiols. Shows superior fluorescence and water solubility properties than dansyl chloride.
Zusätzliche Informationen
| Synonyms | N-(7-Dimethylamino-4-methylcoumarin-3-yl) maleimide |
|---|---|
| Purity | ≥98% (NMR) |
| Appearance | Orange solid |
| CAS-Number | 55145-14-7 |
| Molecular Formula | C16H14N2O4 |
| Molecular Weight | 298.29 |
| Identity | 1H-NMR |
| Solvents | water |
| Melting Point | 218-220 °C |
| Fluorescence | λex 398 nm, λem 482 nm in 0.1 M phosphate pH 7.0 (after derivatization with 2-mercaptoethanol) |
| Smiles | CN(C)C1=CC=C2C(OC(=O)C(N3C(=O)C=CC3=O)=C2C)=C1 |
| InChi Key | ADEORFBTPGKHRP-UHFFFAOYSA-N |
| Shipping | AMBIENT |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice | Protect from light and moisture. |
| Use / Stability | Stable for at least 2 years after receipt when stored at -20°C. |
| Hazard statements | H315, H335 |
| Precautionary statements | P261 |
| GHS Symbol | GHS07 |
| Signal word | Warning |
| Transportation | Not dangerous goods |
| References | (1) L. Pollegioni et al.: Arch. of Biochem. Bioph. 343(1), 1 (1997), (2) B. Werneburg et al.: Arch. of Biochem. Bioph. 303(2), 214 (1993), (3) Y. Kanaoka et al.: Angew. Chem. 89, 141 (1977) |
| InChi | InChI=1S/C16H14N2O4/c1-9-11-5-4-10(17(2)3)8-12(11)22-16(21)15(9)18-13(19)6-7-14(18)20/h4-8H,1-3H3 |
| Quantity | 5 mg, 25 mg, Bulk |
