Specifications
- Purity
- ≥98% (HPLC)
- Appearance
- Clear colorless to yellow liquid
- Identity
- 1H-NMR
Properties
- Solvents
- DMSO
- Density
- 1.100 g/cm3
Categories: Fluorescent Detection, Fluorescent Detection
DAF-FM is a highly sensitive, photo-stable fluorescent probe for the detection of nitric oxide (NO). DAF-2 is the original compound and is widely used, but DAF-FM is more sensitive to nitric oxide (NO) compared with DAF-2. DAF-FM (Ex: 500 nm, Em: 515 nm, dectection limit: 3 nM) is more photo-stable and less pH sensitive than DAF-2. DAF-FM is not quite as cell-permeable as the diacetate derivative, but it does not need to be activated by cytosolic enzymes.
Synonyms | 4-Amino-5-methylamino- 2',7'-difluorofluorescein, Diaminofluorescein-FM |
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Purity | ≥98% (HPLC) |
Appearance | Clear colorless to yellow liquid |
CAS-Number | 254109-20-1 |
Molecular Formula | C21H14F2N2O5 |
Molecular Weight | 412.34 |
Identity | 1H-NMR |
Solvents | DMSO |
Density | 1.100 g/cm3 |
Smiles | O=C1C=C2OC3=C(C(C4=C(C(O)=O)C(N)=C(NC)C=C4)=C2C=C1F)C=C(F)C(O)=C3 |
InChi Key | BJTLSPOVXMBXRZ-UHFFFAOYSA-N |
Shipping | AMBIENT |
Short Term Storage | +4°C |
Long Term Storage | -20°C |
Handling Advice | Protect from light and moisture. |
Use / Stability | Stable for at least 2 years after receipt when stored at -20°C. |
RIDADR | UN1993 |
Transportation | Packing Group III |
References | (1) W.S. Kim et al., Anal. Chem. 78(6), 1859 (2006), (2) M.M. Knight et al., Am. J. Physiol. 284(4, Pt. 1), C1083 (2003), (3) H. Kojima et al., Adv. Mat. 12(10), 763 (2002), (4) H. Kojima et al., Angew. Chem. 111( 21), 3419 (1999) |
InChi | InChI=1S/C21H14F2N2O5/c1-25-13-3-2-8(19(20(13)24)21(28)29)18-9-4-11(22)14(26)6-16(9)30-17-7-15(27)12(23)5-10(17)18/h2-7,25-26H,24H2,1H3,(H,28,29) |
Quantity | 1 mg, Bulk |