Available from stock

D,L-Luciferin 6′-methyl ether

SKU: L0316

Category: Non Fluorescent Detection & Chromogene Detection

Synonyms: 6'-Methoxyluciferin; 3022-11-5
CAS-Number: C₁₂H₁₀N₂O₃S₂
Molecular Formula: 294.35
Molecular Weight

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Product Data
Handling
Safety Information
Details

Specifications

Purity: ≥98% (HPLC)
Appearance: White to faint yellow powder
Identity: 1H-NMR

Properties

Solvents: Soluble in DMSO or methanol.

Shipping: AMBIENT
Short Term Storage: +4°C
Long Term Storage: -20°C
Handling Advice: Very light-sensitive. Protect from light and moisture.
Use / Stability: Stable for at least 2 years after receipt when stored at -20°C.

Transportation: Not dangerous goods

Description: D,L-Luciferin 6′-methyl ether is an analog of Luciferin in which the 6′-hydroxyl group is methylated. This structural modification significantly reduces productive oxidation by firefly luciferase while retaining enzyme binding affinity. As a result, the compound functions as a non-productive substrate analog that can competitively interfere with D-luciferin-dependent bioluminescence. D,L-Luciferin 6′-methyl ether is used in mechanistic enzymology and chemical biology to investigate luciferase substrate recognition, active-site interactions, and structure-activity relationships. It is also valuable for the evaluation of engineered or mutant luciferases with altered substrate specificity. Spectral Data: Excitation max: ~ 326nm, Emission max: ~430nm (accord. Lit).
References: [1] E.H. White, et al.; J. Org. Chem. 30, 2344 (1965) , [2] E.H. White, et al.; Bioorg. Cherm. 1, 92 (1971) , [3] O.A. Gandelman, et al.; J. Photochem. Photobiol. B. Biol. 19, 187 (1993) , [4] G. Meroni, et al.; Arkivoc 265-288 (2009) (Review)

D,L-Luciferin 6′-methyl ether is an analog of Luciferin in which the 6′-hydroxyl group is methylated. This structural modification significantly reduces productive oxidation by firefly luciferase while retaining enzyme binding affinity. As a result, the compound functions as a non-productive substrate analog that can competitively interfere with D-luciferin-dependent bioluminescence. D,L-Luciferin 6′-methyl ether is used in mechanistic enzymology and chemical biology to investigate luciferase substrate recognition, active-site interactions, and structure-activity relationships. It is also valuable for the evaluation of engineered or mutant luciferases with altered substrate specificity. Spectral Data: Excitation max: ~ 326nm, Emission max: ~430nm (accord. Lit).

SKU: L0316 Category: Non Fluorescent Detection & Chromogene Detection

Additional information

Additional information

Synonyms	6'-Methoxyluciferin
Purity	≥98% (HPLC)
Appearance	White to faint yellow powder
CAS-Number	3022-11-5
Molecular Formula	C12H10N2O3S2
Molecular Weight	294.35
Identity	1H-NMR
Solvents	Soluble in DMSO or methanol.
Shipping	AMBIENT
Short Term Storage	+4°C
Long Term Storage	-20°C
Handling Advice	Very light-sensitive. Protect from light and moisture.
Use / Stability	Stable for at least 2 years after receipt when stored at -20°C.
Transportation	Not dangerous goods
References	[1] E.H. White, et al.; J. Org. Chem. 30, 2344 (1965), [2] E.H. White, et al.; Bioorg. Cherm. 1, 92 (1971), [3] O.A. Gandelman, et al.; J. Photochem. Photobiol. B. Biol. 19, 187 (1993), [4] G. Meroni, et al.; Arkivoc 265-288 (2009) (Review)
Quantity	50 mg, 250 mg, Bulk