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    Indo 1-AM

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      Indo 1-AM

      SKU
      I0019

      Category: Fluorescent Detection

      Synonyms
      Indo-1-(acetoxymethyl) ester , 4-(6-Carboxy-2-indolyl)-4'-methyl-2,2'-(ethylenedioxy)dianiline-N,N,N',N'-tetraacetic acid tetrakis(acetoxymethyl) ester
      112926-02-0
      CAS-Number
      C47H51N3O22
      Molecular Formula
      1009.93
      Molecular Weight

      • Product Data
      • Handling
      • Safety Information
      • Details

      Specifications

      Purity
      ≥90% (HPLC)
      Appearance
      Off-white solid
      Purity 2
      ≥90% (TLC)

      Properties

      Solvents
      DMSO, methanol
      Long Term Storage
      -20°C
      Use / Stability
      Stable for at least 2 years after receipt when stored at -20°C.
      Handling Advice
      Protect from light and moisture.
      Short Term Storage
      -20°C
      Shipping
      AMBIENT
      Transportation
      Not dangerous goods
      Description
      INDO 1/AM is a popular non-invasive UV-excitable calcium indicator. It is the cell-permeable ester derivative of INDO 1. After crossing the cell membrane, INDO 1/AM is rapidly hydrolyzed by cytoplasmic esterases to produce the ratiometric fluorescent calcium indicator INDO 1, which remains trapped within the cell. INDO 1/AM has been used to selectively monitor Ca2+ levels in mitochondria and in the cytosol. INDO 1 is ideal for analyses using flow cytometry, as it uses a single excitation source (usually the 351-364nm spectral lines of the argon-ion laser). In contrast to FURA 1, INDO 1 has a dual emission peak. The emission of INDO 1 shifts to 400nm (when bound to Ca2+) from 480nm in Ca2+-free environments. INDO 1 is prone to photobleaching, which limits its usefulness in methods involving microscopy. Calcium measurement is critical for numerous biological investigations. Fluorescent probes that show spectral responses upon binding Ca2+ have enabled researchers to investigate changes in intracellular free Ca2+ concentrations by using fluorescence microscopy, flow cytometry, fluorescence spectroscopy and fluorescence microplate readers. Spectral Data: λex 330nm, λem 450nm (with Calcium), λex 356nm, λem 478nm in methanol.
      InChi
      InChI=1S/C47H51N3O22/c1-28-7-11-39(49(19-43(56)68-23-63-29(2)51)20-44(57)69-24-64-30(3)52)41(15-28)61-13-14-62-42-18-35(37-16-34-8-9-36(17-38(34)48-37)47(60)72-27-67-33(6)55)10-12-40(42)50(21-45(58)70-25-65-31(4)53)22-46(59)71-26-66-32(5)54/h7-12,15-18,48H,13-14,19-27H2,1-6H3
      References
      (1) G. Grynkiewicz, et al., J. Biol. Chem. 260, 3440 (1985) , (2) M. Lopez, et al., Cytometry 10, 165 (1989) , (3) J.P. Grierson, et al., J. Neurophysiol. 67, 704 (1992) , (4) E.J. Griffiths, et al., Am. J. Physiol. 273, C37 (1997) , (5) R.M. Paredes, et al., Meth. A Comp. Meth. Enzymol. 46, 143 (2008) , (6) Z. Zhou, et al., J. Physiol. 507, 379 (1998) , (7) S. Bailey & P.J. Macardle, J. Immunol. Meth. 311, 220 (2006) , (8) A. Nelemans, Methods Mol. Biol. 312, 47 (2006) , (9) R.W. Sabnis, Handbook of biological dyes and stains (2010)
      InChi Key
      CAWBRCOBJNWRLK-UHFFFAOYSA-N
      Smiles
      CC1=CC(OCCOC2=CC(C3=CC(C=CC(C(OCOC(C)=O)=O)=C4)=C4N3)=CC=C2N(CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O)=C(N(CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O)C=C1

      INDO 1/AM is a popular non-invasive UV-excitable calcium indicator. It is the cell-permeable ester derivative of INDO 1. After crossing the cell membrane, INDO 1/AM is rapidly hydrolyzed by cytoplasmic esterases to produce the ratiometric fluorescent calcium indicator INDO 1, which remains trapped within the cell. INDO 1/AM has been used to selectively monitor Ca2+ levels in mitochondria and in the cytosol. INDO 1 is ideal for analyses using flow cytometry, as it uses a single excitation source (usually the 351-364nm spectral lines of the argon-ion laser). In contrast to FURA 1, INDO 1 has a dual emission peak. The emission of INDO 1 shifts to 400nm (when bound to Ca2+) from 480nm in Ca2+-free environments. INDO 1 is prone to photobleaching, which limits its usefulness in methods involving microscopy. Calcium measurement is critical for numerous biological investigations. Fluorescent probes that show spectral responses upon binding Ca2+ have enabled researchers to investigate changes in intracellular free Ca2+ concentrations by using fluorescence microscopy, flow cytometry, fluorescence spectroscopy and fluorescence microplate readers. Spectral Data: λex 330nm, λem 450nm (with Calcium), λex 356nm, λem 478nm in methanol.

      Artikelnummer: I0019 Kategorie: Fluorescent Detection
      • Zusätzliche Information

      Zusätzliche Information

      Synonyms

      Indo-1-(acetoxymethyl) ester, 4-(6-Carboxy-2-indolyl)-4'-methyl-2,2'-(ethylenedioxy)dianiline-N,N,N',N'-tetraacetic acid tetrakis(acetoxymethyl) ester

      Purity

      ≥90% (HPLC)

      Appearance

      Off-white solid

      CAS-Number

      112926-02-0

      Molecular Formula

      C47H51N3O22

      Molecular Weight

      1009.93

      Solvents

      DMSO, methanol

      Smiles

      CC1=CC(OCCOC2=CC(C3=CC(C=CC(C(OCOC(C)=O)=O)=C4)=C4N3)=CC=C2N(CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O)=C(N(CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O)C=C1

      InChi Key

      CAWBRCOBJNWRLK-UHFFFAOYSA-N

      Shipping

      AMBIENT

      Short Term Storage

      -20°C

      Long Term Storage

      -20°C

      Handling Advice

      Protect from light and moisture.

      Use / Stability

      Stable for at least 2 years after receipt when stored at -20°C.

      Transportation

      Not dangerous goods

      References

      (1) G. Grynkiewicz, et al., J. Biol. Chem. 260, 3440 (1985), (2) M. Lopez, et al., Cytometry 10, 165 (1989), (3) J.P. Grierson, et al., J. Neurophysiol. 67, 704 (1992), (4) E.J. Griffiths, et al., Am. J. Physiol. 273, C37 (1997), (5) R.M. Paredes, et al., Meth. A Comp. Meth. Enzymol. 46, 143 (2008), (6) Z. Zhou, et al., J. Physiol. 507, 379 (1998), (7) S. Bailey & P.J. Macardle, J. Immunol. Meth. 311, 220 (2006), (8) A. Nelemans, Methods Mol. Biol. 312, 47 (2006), (9) R.W. Sabnis, Handbook of biological dyes and stains (2010)

      InChi

      InChI=1S/C47H51N3O22/c1-28-7-11-39(49(19-43(56)68-23-63-29(2)51)20-44(57)69-24-64-30(3)52)41(15-28)61-13-14-62-42-18-35(37-16-34-8-9-36(17-38(34)48-37)47(60)72-27-67-33(6)55)10-12-40(42)50(21-45(58)70-25-65-31(4)53)22-46(59)71-26-66-32(5)54/h7-12,15-18,48H,13-14,19-27H2,1-6H3

      Quantity

      1 mg, Bulk

      cdx-Purity 2

      ≥90% (TLC)

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