Available from stock

Methyl Green zinc chloride salt

SKU: M0641

Category: Non Fluorescent Detection & Chromogene Detection

Synonyms: Ethyl Green , CI 42590; 7114-03-6
CAS-Number: C₂₇H₃₅BrClN₃ · ZnCl₂
Molecular Formula: 653.24
Molecular Weight

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Product Data
Handling
Safety Information
Details

Specifications

Purity: ≥80% (Dye content)
Appearance: Dark red to brown to green powder
Impurities: ≤0.5% crystal violet

Properties

Solvents: water (1 mg/ml), ethanol, methanol or DMSO.

Shipping: AMBIENT
Short Term Storage: RT
Long Term Storage: RT
Handling Advice: Protect from light and moisture.
Use / Stability: Stable for at least 2 years after receipt when stored at RT.

Hazard statements: H314 - H411
Precautionary statements: P273 - P280 - P305 + P351 + P338 + P310 - P391 - P501
GHS Symbol: GHS05+GHS09
Signal word: Danger
Transportation: Not dangerous goods

Description: Marker dye for DNA in acid and neutral media. Methyl green is mainly used as a DNA stain in histochemistry. At low concentrations it binds selectively to AT-rich regions of native DNA. At high concentrations it is present at all available DNA sites. Methyl Green is used as a tracking dye in acid buffers for electrophoresis. It helps differentiate bacteria and is also a tissue stain for nucleoli and mitochondria in tissues. Spectral data: Absorption maxima = 630-635nm. May be used as a pH indicator with a pH range = pH 0.2(yellow) to pH 1.8(blue).
Smiles: Cl[Zn]Cl.CN(C)C1=CC=C(/C(C2=CC=C([N+](C)(CC)C)C=C2)=C3C=C/C(C=C/3)=[N+](C)/C)C=C1.[Br-].[Cl-]
InChi Key: RRKSQWOQZFIQKW-UHFFFAOYSA-J
References: (1) H. Lyon, et al., Histochem. J. 24, 224 (1992) , (2) S.K. Kim & B. Norden, FEBS Lett. 315, 61-64 (1993) , (3) R.W. Sabnis, Handbook of Acid-Base indicators (2008) , (4) D. Prieto, et al., Histochem. Cell Biol. 142, 335 (2014) , (5) P.Murgai, et al., Anal. Biochem. 601, 113782 (2020)
InChi: InChI=1S/C27H35N3.BrH.3ClH.Zn/c1-8-30(6,7)26-19-13-23(14-20-26)27(21-9-15-24(16-10-21)28(2)3)22-11-17-25(18-12-22)29(4)5;;;;;/h9-20H,8H2,1-7H3;4*1H;/q+2;;;;;+2/p-4

Marker dye for DNA in acid and neutral media. Methyl green is mainly used as a DNA stain in histochemistry. At low concentrations it binds selectively to AT-rich regions of native DNA. At high concentrations it is present at all available DNA sites. Methyl Green is used as a tracking dye in acid buffers for electrophoresis. It helps differentiate bacteria and is also a tissue stain for nucleoli and mitochondria in tissues. Spectral data: Absorption maxima = 630-635nm. May be used as a pH indicator with a pH range = pH 0.2(yellow) to pH 1.8(blue).

SKU: M0641 Category: Non Fluorescent Detection & Chromogene Detection

Additional information

Additional information

Synonyms	Ethyl Green, CI 42590
Purity	≥80% (Dye content)
Appearance	Dark red to brown to green powder
CAS-Number	7114-03-6
Molecular Formula	C27H35BrClN3 · ZnCl2
Molecular Weight	653.24
Solvents	water (1 mg/ml), ethanol, methanol or DMSO.
Smiles	Cl[Zn]Cl.CN(C)C1=CC=C(/C(C2=CC=C([N+](C)(CC)C)C=C2)=C3C=C/C(C=C/3)=[N+](C)/C)C=C1.[Br-].[Cl-]
InChi Key	RRKSQWOQZFIQKW-UHFFFAOYSA-J
Shipping	AMBIENT
Short Term Storage	RT
Long Term Storage	RT
Handling Advice	Protect from light and moisture.
Use / Stability	Stable for at least 2 years after receipt when stored at RT.
Hazard statements	H314 – H411
Precautionary statements	P273 – P280 – P305 + P351 + P338 + P310 – P391 – P501
GHS Symbol	GHS05+GHS09
Signal word	Danger
Transportation	Not dangerous goods
References	(1) H. Lyon, et al., Histochem. J. 24, 224 (1992), (2) S.K. Kim & B. Norden, FEBS Lett. 315, 61-64 (1993), (3) R.W. Sabnis, Handbook of Acid-Base indicators (2008), (4) D. Prieto, et al., Histochem. Cell Biol. 142, 335 (2014), (5) P.Murgai, et al., Anal. Biochem. 601, 113782 (2020)
InChi	InChI=1S/C27H35N3.BrH.3ClH.Zn/c1-8-30(6,7)26-19-13-23(14-20-26)27(21-9-15-24(16-10-21)28(2)3)22-11-17-25(18-12-22)29(4)5;;;;;/h9-20H,8H2,1-7H3;4*1H;/q+2;;;;;+2/p-4
Quantity	5 g, 25 g, Bulk
cdx-Impurities	≤0.5% crystal violet