Available from stock

N-Succinimidyl 3-(2-pyridyldithio)propionate

SKU: S0019

Category: Linker

Synonyms: 3-(2-Pyridyldithio)propionic acid N-hydroxysuccinimide ester, SPDP; 68181-17-9
CAS-Number: C₁₂H₁₂N₂O₄S₂
Molecular Formula: 312.36
Molecular Weight

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Product Data
Handling
Safety Information
Details

Specifications

Purity: ≥97% (NMR)
Appearance: White to off-white powder
Identity: 1H-NMR

Properties

Solvents: acetone (50mg/ml), DMF or methanol
Melting Point: 84-86 °C (lit.)

Documentation

Safety Data Sheet (SDS): CDX S0019 MSDS.pdf

Shipping: AMBIENT
Short Term Storage: +4°C
Long Term Storage: -20°C
Handling Advice: Protect from light and moisture.
Use / Stability: Stable for at least 2 years after receipt when stored at -20°C.

Transportation: Not dangerous goods

Description: N-Succinimidyl 3-(2-pyridyldithio)propionate is a heterobifunctional cross-linking reagent with amine and sulfhydryl reactivity. It is typically coupled initially to molecules containing primary amines by amide bonds buffered at pH 7.5 (6.5-8.5). Second coupling specific for molecules containing free sulfhydryl by thiol-disulfide exchange buffered at pH 8.0 (7.5-8.5). Alternately, this reagent may be used as a protected thiolating reagent following initial coupling and reduction of linker. It incorporates four atom linker. N-Succinimidyl 3-(2-pyridyldithio)propionate (SPDP) is useful for the preparation of enzyme immunoconjugates and hapten carrier molecule conjugates. It has been used for antibody conjugation and as a protein cross-linker. It is also a glutathione cleavable ADC linker used for the antibody-drug conjugates (ADCs).
Smiles: O=C(CCSSC1=NC=CC=C1)ON2C(CCC2=O)=O
InChi Key: JWDFQMWEFLOOED-UHFFFAOYSA-N
References: (1) J. Carlsson, et al., Biochem. J. 173, 723 (1978) , (2) D. Pain & A. Surolia, J. Immunol. Methods 40, 219 (1981) , (3) P. Nilsson, et al., J. Immunol. Methods 41, 81 (1981) , (4) A.F. Habeeb, Biochim. Biophys. Acta 673, 527 (1981) , (5) Y.H. Jou, et al., Immunol. Commun. 11, 357 (1982) , (6) Y.H. Jou, et al., Methods Enzymol. 92, 257 (1983)
InChi: InChI=1S/C12H12N2O4S2/c15-10-4-5-11(16)14(10)18-12(17)6-8-19-20-9-3-1-2-7-13-9/h1-3,7H,4-6,8H2

N-Succinimidyl 3-(2-pyridyldithio)propionate is a heterobifunctional cross-linking reagent with amine and sulfhydryl reactivity. It is typically coupled initially to molecules containing primary amines by amide bonds buffered at pH 7.5 (6.5-8.5). Second coupling specific for molecules containing free sulfhydryl by thiol-disulfide exchange buffered at pH 8.0 (7.5-8.5). Alternately, this reagent may be used as a protected thiolating reagent following initial coupling and reduction of linker. It incorporates four atom linker. N-Succinimidyl 3-(2-pyridyldithio)propionate (SPDP) is useful for the preparation of enzyme immunoconjugates and hapten carrier molecule conjugates. It has been used for antibody conjugation and as a protein cross-linker. It is also a glutathione cleavable ADC linker used for the antibody-drug conjugates (ADCs).

Artikelnummer: S0019 Kategorie: Linker

Zusätzliche Informationen

Zusätzliche Informationen

Synonyms	3-(2-Pyridyldithio)propionic acid N-hydroxysuccinimide ester, SPDP
Purity	≥97% (NMR)
Appearance	White to off-white powder
CAS-Number	68181-17-9
Molecular Formula	C12H12N2O4S2
Molecular Weight	312.36
Identity	1H-NMR
Solvents	acetone (50mg/ml), DMF or methanol
Melting Point	84-86 °C (lit.)
Smiles	O=C(CCSSC1=NC=CC=C1)ON2C(CCC2=O)=O
InChi Key	JWDFQMWEFLOOED-UHFFFAOYSA-N
Shipping	AMBIENT
Short Term Storage	+4°C
Long Term Storage	-20°C
Handling Advice	Protect from light and moisture.
Use / Stability	Stable for at least 2 years after receipt when stored at -20°C.
Transportation	Not dangerous goods
References	(1) J. Carlsson, et al., Biochem. J. 173, 723 (1978), (2) D. Pain & A. Surolia, J. Immunol. Methods 40, 219 (1981), (3) P. Nilsson, et al., J. Immunol. Methods 41, 81 (1981), (4) A.F. Habeeb, Biochim. Biophys. Acta 673, 527 (1981), (5) Y.H. Jou, et al., Immunol. Commun. 11, 357 (1982), (6) Y.H. Jou, et al., Methods Enzymol. 92, 257 (1983)
InChi	InChI=1S/C12H12N2O4S2/c15-10-4-5-11(16)14(10)18-12(17)6-8-19-20-9-3-1-2-7-13-9/h1-3,7H,4-6,8H2
Quantity	25 mg, 100 mg, Bulk