Specifications
- Purity
- ≥99% (HPLC)
- Appearance
- White to off-white powder
- Identity
- 1H-NMR
Properties
- Solvents
- Soluble in ethanol (50mg/ml).
Available from stock
Naphthol AS phosphate
- SKU
- N0130
Category: Non Fluorescent Detection & Chromogene Detection
- Synonyms
- 3-Hydroxy-2-naphthanilide phosphate, HNPP
- 13989-98-5
- CAS-Number
- C17H14NO5P
- Molecular Formula
- 343.27
- Molecular Weight
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
- Shipping
- AMBIENT
- Short Term Storage
- +4°C
- Long Term Storage
- -20°C
- Handling Advice
- Very hygroscopic. Store under inert gas.
- Use / Stability
- Stable for at least 2 years after receipt when stored at -20°C.
- Transportation
- Not dangerous goods
- Description
- Naphthol AS phosphate is a fluorogenic substrate for acid and alkaline phosphatases. Naphthol AS phosphate is converted to naphthol AS displaying excitation/emission spectra of 388/516nm. The fluorescence is a quantitative marker of acid and alkaline phosphatase activity. Acid and alkaline phosphatases are often used as clinical markers of disease, since the concentration of human acid and alkaline phosphatases undergo pronounced changes in particular diseases, resulting in unusually high or low concentrations. It is a histochemical substrate commonly used in conjunction with diazonium salts to form a specific azo dye for immunohistology, immunoblotting, dot blot applications and in situ PCR.
- Smiles
- O=C(NC1=CC=CC=C1)C2=CC3=CC=CC=C3C=C2OP(O)(O)=O
- InChi Key
- KVIYXIWBXOQZDN-UHFFFAOYSA-N
- References
- (1) Z. Lojda, etal., Histochemie 11, 13 (1967) , (2) A. Vaughan, et al., Anal. Chem. 43, 721 (1971) , (3) S. West, et al., Anal. Biochem. 190, 254 (1990) , (4) H.J. Hoeltke, et al., Anal. Biochem. 207, 24 (1992) , (5) K. Tani, et al., Appl. Environ. Microbiol. 64, 1536 (1998)
Naphthol AS phosphate is a fluorogenic substrate for acid and alkaline phosphatases. Naphthol AS phosphate is converted to naphthol AS displaying excitation/emission spectra of 388/516nm. The fluorescence is a quantitative marker of acid and alkaline phosphatase activity. Acid and alkaline phosphatases are often used as clinical markers of disease, since the concentration of human acid and alkaline phosphatases undergo pronounced changes in particular diseases, resulting in unusually high or low concentrations. It is a histochemical substrate commonly used in conjunction with diazonium salts to form a specific azo dye for immunohistology, immunoblotting, dot blot applications and in situ PCR.
Additional information
| Synonyms | 3-Hydroxy-2-naphthanilide phosphate, HNPP |
|---|---|
| Purity | ≥99% (HPLC) |
| Appearance | White to off-white powder |
| CAS-Number | 13989-98-5 |
| Molecular Formula | C17H14NO5P |
| Molecular Weight | 343.27 |
| Identity | 1H-NMR |
| Solvents | Soluble in ethanol (50mg/ml). |
| Smiles | O=C(NC1=CC=CC=C1)C2=CC3=CC=CC=C3C=C2OP(O)(O)=O |
| InChi Key | KVIYXIWBXOQZDN-UHFFFAOYSA-N |
| Shipping | AMBIENT |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice | Very hygroscopic. Store under inert gas. |
| Use / Stability | Stable for at least 2 years after receipt when stored at -20°C. |
| Transportation | Not dangerous goods |
| References | (1) Z. Lojda, etal., Histochemie 11, 13 (1967), (2) A. Vaughan, et al., Anal. Chem. 43, 721 (1971), (3) S. West, et al., Anal. Biochem. 190, 254 (1990), (4) H.J. Hoeltke, et al., Anal. Biochem. 207, 24 (1992), (5) K. Tani, et al., Appl. Environ. Microbiol. 64, 1536 (1998) |
| Quantity | 500 mg, 1 g, Bulk |
