Available from stock

Tetrazolium Blue Chloride

SKU: T0136

Category: Non Fluorescent Detection & Chromogene Detection

Synonyms: BTC, Blue Tetrazolium chloride, TZ, NSC 27623, Ditetrazolium chloride, 3,3'-Dianisole-4,4'-bis(3,5-diphenyltetrazolium chloride); 1871-22-3
CAS-Number: C₄₀H₃₂Cl₂N₈O₂
Molecular Formula: 727.65
Molecular Weight

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Product Data
Handling
Safety Information
Details

Specifications

Purity: ≥97% (HPLC)
Appearance: Yellow crystalline solid
Identity: 1H-NMR

Properties

Solvents: Soluble in methanol (50mg/ml).
Melting Point: 255 °C (dec.)(lit.)
Density: 1.9137 (rough estimate)

Shipping: AMBIENT
Short Term Storage: +4°C
Long Term Storage: -20°C
Handling Advice: Protect from light and moisture.
Use / Stability: Stable for at least 6 months after receipt when stored at -20°C.

Hazard statements: H350
Precautionary statements: P201, P308+P313
GHS Symbol: GHS08
Signal word: Danger
Transportation: Not dangerous goods

Description: Tetrazolium Blue Chloride (Nitro Blue Tetrazolium, NBT) is a redox-sensitive dye widely used as a chromogenic reagent for detecting enzymatic activity, reactive oxygen species (ROS), and cellular respiration. Upon reduction by dehydrogenases or superoxide radicals, it forms an insoluble blue formazan precipitate, enabling visual or spectrophotometric quantification of metabolic activity. NBT is commonly used as a colorimetric reagent and applied in microbiology, histochemistry, and plant and animal cell research to assess oxidative burst, mitochondrial function, and electron transport chain activity. Spectral Data: λabs ~540 nm, with a wide wavelength range of 480-600 nm.
Smiles: COC1=CC(C2=CC(OC)=C(N3N=C(C4=CC=CC=C4)N=[N+]3C5=CC=CC=C5)C=C2)=CC=C1N6[N+](C7=CC=CC=C7)=NC(C8=CC=CC=C8)=N6.[Cl-].[Cl-]
InChi Key: MUUHXGOJWVMBDY-UHFFFAOYSA-L
References: (1) V.A. DeBari &M.A. Needle; J. Immunol. Meth. 40, 89 (1981) , (2) C.K. Jue & P.N. Lipke; J. Biochem. Biophys. Meth. 11, 109 (1985) , (3) M.V. Berridge, et al.; Biotechnol. Annu. Rev. 11, 127 (2005) , (4) L.J. Yan, et al.; Electrophoresis 28, 1036 (2007) , (5) C. Pannecouque, et al.; Nat. Protoc. 3, 427 (2008) , (6) R.W. Sabnis; Handbook of biological dyes and stains (2010)
InChi: InChI=1S/C40H32N8O2.2ClH/c1-49-37-27-31(23-25-35(37)47-43-39(29-15-7-3-8-16-29)41-45(47)33-19-11-5-12-20-33)32-24-26-36(38(28-32)50-2)48-44-40(30-17-9-4-10-18-30)42-46(48)34-21-13-6-14-22-34;;/h3-28H,1-2H3;2*1H/q+2;;/p-2

Tetrazolium Blue Chloride (Nitro Blue Tetrazolium, NBT) is a redox-sensitive dye widely used as a chromogenic reagent for detecting enzymatic activity, reactive oxygen species (ROS), and cellular respiration. Upon reduction by dehydrogenases or superoxide radicals, it forms an insoluble blue formazan precipitate, enabling visual or spectrophotometric quantification of metabolic activity. NBT is commonly used as a colorimetric reagent and applied in microbiology, histochemistry, and plant and animal cell research to assess oxidative burst, mitochondrial function, and electron transport chain activity. Spectral Data: λabs ~540 nm, with a wide wavelength range of 480-600 nm.

SKU: T0136 Category: Non Fluorescent Detection & Chromogene Detection

Additional information

Additional information

Synonyms	BTC, Blue Tetrazolium chloride, TZ, NSC 27623, Ditetrazolium chloride, 3,3'-Dianisole-4,4'-bis(3,5-diphenyltetrazolium chloride)
Purity	≥97% (HPLC)
Appearance	Yellow crystalline solid
CAS-Number	1871-22-3
Molecular Formula	C40H32Cl2N8O2
Molecular Weight	727.65
Identity	1H-NMR
Solvents	Soluble in methanol (50mg/ml).
Melting Point	255 °C (dec.)(lit.)
Density	1.9137 (rough estimate)
Smiles	COC1=CC(C2=CC(OC)=C(N3N=C(C4=CC=CC=C4)N=[N+]3C5=CC=CC=C5)C=C2)=CC=C1N6[N+](C7=CC=CC=C7)=NC(C8=CC=CC=C8)=N6.[Cl-].[Cl-]
InChi Key	MUUHXGOJWVMBDY-UHFFFAOYSA-L
Shipping	AMBIENT
Short Term Storage	+4°C
Long Term Storage	-20°C
Handling Advice	Protect from light and moisture.
Use / Stability	Stable for at least 6 months after receipt when stored at -20°C.
Hazard statements	H350
Precautionary statements	P201, P308+P313
GHS Symbol	GHS08
Signal word	Danger
Transportation	Not dangerous goods
References	(1) V.A. DeBari &M.A. Needle; J. Immunol. Meth. 40, 89 (1981), (2) C.K. Jue & P.N. Lipke; J. Biochem. Biophys. Meth. 11, 109 (1985), (3) M.V. Berridge, et al.; Biotechnol. Annu. Rev. 11, 127 (2005), (4) L.J. Yan, et al.; Electrophoresis 28, 1036 (2007), (5) C. Pannecouque, et al.; Nat. Protoc. 3, 427 (2008), (6) R.W. Sabnis; Handbook of biological dyes and stains (2010)
InChi	InChI=1S/C40H32N8O2.2ClH/c1-49-37-27-31(23-25-35(37)47-43-39(29-15-7-3-8-16-29)41-45(47)33-19-11-5-12-20-33)32-24-26-36(38(28-32)50-2)48-44-40(30-17-9-4-10-18-30)42-46(48)34-21-13-6-14-22-34;;/h3-28H,1-2H3;2*1H/q+2;;/p-2
Quantity	1 g, 5 g, 25 g, Bulk