Specifications
- Purity
- ≥98% (HPLC)
- Appearance
- Dark purple solid
- Identity
- 1H-NMR
Properties
- Solvents
- DMSO
- Melting Point
- 255-257°C, 241-243°C, 223 °C (decomp)
Available from stock
1,1′-Dimethyl-3,3,3′,3′-tetramethyl indocarbocyanine iodide
- SKU
- D1024
Category: Fluorescent Detection
- Synonyms
- DiIC1(3), HICI, NK 79, 1,1?,3,3,3?,3?-Hexamethylindocarbocyanine iodide, Cy3 dimethyl,
- 25470-94-4
- CAS-Number
- C25H29IN2
- Molecular Formula
- 484.43
- Molecular Weight
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
- Shipping
- AMBIENT
- Short Term Storage
- +4°C
- Long Term Storage
- -20°C
- Handling Advice
- Protect from light and air. Store under inert gas.
- Use / Stability
- Stable for at least 2 years after receipt when stored at -20°C.
- Hazard statements
- H315-H319-H335
- Precautionary statements
- P261-P264-P271-P280-P302+P352-P304+P340-P305+P351+P338-P312-P362-P403+P233-P501
- GHS Symbol
- GHS07
- Signal word
- Warning
- Transportation
- Not dangerous goods
- Description
- DiIC1(3) is a fluorescent carbocyanine dye used mainly for assessing membrane potential in mitochondria and plasma membranes, especially in flow cytometry and fluorescence microscopy. DiIC1(3) has short alkyl chains (methyl groups), so it diffuses more easily but is less stably anchored in membranes than longer-chain versions like DiIC12(3) or DiIC18(3). It is ideal for dynamic membrane potential assessments, not long-term labeling. The dye can be used as a non-reactive fluorophore, for control experiments and for calibration. Spectral Data: Excitation max: ~ 555nm, Emission max: ~570nm (accord. Lit).
- Smiles
- CN1C=2C(C(C)(C)C1=CC=CC=3C(C)(C)C=4C([N+]3C)=CC=CC4)=CC=CC2.[I-]
- InChi Key
- ZGBJQZCIUVDAHE-UHFFFAOYSA-M
- References
- (1) P.M.W. French & J.R. Taylor, Opt. Lett. 11, 297 (1986) , (2) H. Sato, et al., J. Raman Spectr. 19, 129 (1988) , (3) A.S. Tatikolov, et al., Chem. Phys. Lett. 190, 291 (1992) , (4) Z.-H. Peng, et al., J. Mater. Chem. 6, 559 (1996) , (5) A.L. Mikheikin, et al., J. Biomol. Struct. Dyn. 18, 59 (2000) , (6) H.M. Shapiro, Cytometry 43, 223 (2001) , (7) M. Matsui, et al., Tetrahedr. 71, 3528 (2015)
- InChi
- InChI=1S/C25H29N2.HI/c1-24(2)18-12-7-9-14-20(18)26(5)22(24)16-11-17-23-25(3,4)19-13-8-10-15-21(19)27(23)6,/h7-17H,1-6H3,1H/q+1,/p-1
DiIC1(3) is a fluorescent carbocyanine dye used mainly for assessing membrane potential in mitochondria and plasma membranes, especially in flow cytometry and fluorescence microscopy. DiIC1(3) has short alkyl chains (methyl groups), so it diffuses more easily but is less stably anchored in membranes than longer-chain versions like DiIC12(3) or DiIC18(3). It is ideal for dynamic membrane potential assessments, not long-term labeling. The dye can be used as a non-reactive fluorophore, for control experiments and for calibration. Spectral Data: Excitation max: ~ 555nm, Emission max: ~570nm (accord. Lit).
Additional information
| Synonyms | DiIC1(3), HICI, NK 79, 1,1?,3,3,3?,3?-Hexamethylindocarbocyanine iodide, Cy3 dimethyl, |
|---|---|
| Purity | ≥98% (HPLC) |
| Appearance | Dark purple solid |
| CAS-Number | 25470-94-4 |
| Molecular Formula | C25H29IN2 |
| Molecular Weight | 484.43 |
| Identity | 1H-NMR |
| Solvents | DMSO |
| Melting Point | 255-257°C, 241-243°C, 223 °C (decomp) |
| Smiles | CN1C=2C(C(C)(C)C1=CC=CC=3C(C)(C)C=4C([N+]3C)=CC=CC4)=CC=CC2.[I-] |
| InChi Key | ZGBJQZCIUVDAHE-UHFFFAOYSA-M |
| Shipping | AMBIENT |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice | Protect from light and air. Store under inert gas. |
| Use / Stability | Stable for at least 2 years after receipt when stored at -20°C. |
| Hazard statements | H315-H319-H335 |
| Precautionary statements | P261-P264-P271-P280-P302+P352-P304+P340-P305+P351+P338-P312-P362-P403+P233-P501 |
| GHS Symbol | GHS07 |
| Signal word | Warning |
| Transportation | Not dangerous goods |
| References | (1) P.M.W. French & J.R. Taylor, Opt. Lett. 11, 297 (1986), (2) H. Sato, et al., J. Raman Spectr. 19, 129 (1988), (3) A.S. Tatikolov, et al., Chem. Phys. Lett. 190, 291 (1992), (4) Z.-H. Peng, et al., J. Mater. Chem. 6, 559 (1996), (5) A.L. Mikheikin, et al., J. Biomol. Struct. Dyn. 18, 59 (2000), (6) H.M. Shapiro, Cytometry 43, 223 (2001), (7) M. Matsui, et al., Tetrahedr. 71, 3528 (2015) |
| InChi | InChI=1S/C25H29N2.HI/c1-24(2)18-12-7-9-14-20(18)26(5)22(24)16-11-17-23-25(3,4)19-13-8-10-15-21(19)27(23)6,/h7-17H,1-6H3,1H/q+1,/p-1 |
| Quantity | 50 mg, 100 mg, 250 mg, Bulk |
