Specifications
- Purity
- ≥98% (HPLC)
- Appearance
- Purple crystalline solid
- Identity
- 1H-NMR
Properties
- Solvents
- DMSO or ethanol
Category: Fluorescent Detection
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
DiIC1(3) is a fluorescent carbocyanine dye used mainly for assessing membrane potential in mitochondria and plasma membranes, especially in flow cytometry and fluorescence microscopy. Can also be used as a laser dye. DiIC1(3) has short alkyl chains (methyl groups), so it diffuses more easily but is less stably anchored in membranes than longer-chain versions like DiIC12(3) or DiIC18(3). It is ideal for dynamic membrane potential assessments, not long-term labeling. The dye can be used as a non-reactive fluorophore, for control experiments and for calibration. The BF4 anion is better for organic solvents. Spectral Data: Excitation max: ~ 545nm, Emission max: ~565nm (EtOH).
| Synonyms | DiIC1(3), HIC, 1,3,3,1?,3?,3?-Hexamethylindocarbocyanine tetrafluoroborate |
|---|---|
| Purity | ≥98% (HPLC) |
| Appearance | Purple crystalline solid |
| CAS-Number | 61575-72-2 |
| Molecular Formula | C25H29N2.BF4 |
| Molecular Weight | 444.32 |
| Identity | 1H-NMR |
| Solvents | DMSO or ethanol |
| Smiles | [B-](F)(F)(F)F.CC1(C2=CC=CC=C2[N+](=C1/C=C/C=C\3/C(C4=CC=CC=C4N3C)(C)C)C)C |
| InChi Key | QXPZINFOKFHGAQ-UHFFFAOYSA-N |
| Shipping | AMBIENT |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice | Protect from light and air. Store under inert gas. |
| Use / Stability | Stable for at least 2 years after receipt when stored at -20°C. |
| Hazard statements | H315-H319-H335 |
| Precautionary statements | P261-P264-P271-P280-P302+P352-P304+P340-P305+P351+P338-P312-P362-P403+P233-P501 |
| GHS Symbol | GHS07 |
| Signal word | Warning |
| Transportation | Not dangerous goods |
| References | (1) A.S. Tatikolov, et al., Chem. Phys. Lett. 190, 291 (1992), (2) N.A. Davidenko, et al., Theoret. Exp. Chem. 34, 158 (1998), (3) N.A. Davidenko, et al., Phys. Solid State 41, 179 (1999), (4) N.K. Ibrayev, et al., J. Luminesc. 90, 81 (2000), (5) R.A. Ganeev, et al., Appl. Phys. B 76, 683 (2003), (6) N.A. Davidenko, et al., Spectrochim. Acta Part A 61, 213 (2005), (7) V.A. Svetlichnyi, et al., Quantum Electronics 37, 118 (2007), (8) A.R. Shabelko, et al., Spectrochim. Acta Part A 321, 124728 (2024) |
| InChi | InChI=1S/C25H29N2.BF4/c1-24(2)18-12-7-9-14-20(18)26(5)22(24)16-11-17-23-25(3,4)19-13-8-10-15-21(19)27(23)6,2-1(3,4)5/h7-17H,1-6H3,/q+1,-1 |
| Quantity | 50 mg, 100 mg, 250 mg, Bulk |

1,1′-Dimethyl-3,3,3′,3′-tetramethyl indocarbocyanine perc...