Available from stock

1,1′-Dioctadecyl-3,3,3′,3′-tetramethyl indotricarbocyanine iodide

SKU: D1020

Category: Fluorescent Detection

Synonyms: DiIC18(7), DiR, Cy7 DiC18; 100068-60-8
CAS-Number: C₆₃H₁₀₁N₂.I
Molecular Formula: 1013.40
Molecular Weight

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Product Data
Handling
Safety Information
Details

Specifications

Purity: ≥97% (HPLC)
Appearance: Dark purple solid
Identity: 1H-NMR

Properties

Solvents: DMSO or methanol

Shipping: AMBIENT
Short Term Storage: +4°C
Long Term Storage: -20°C
Handling Advice: Protect from light and air. Store under inert gas.
Use / Stability: Stable for at least 2 years after receipt when stored at -20°C.

Hazard statements: H315-H319-H335
Precautionary statements: P261-P264-P271-P280-P302+P352-P304+P340-P305+P351+P338-P312-P362-P403+P233-P501
GHS Symbol: GHS07
Signal word: Warning
Transportation: Not dangerous goods

Description: DiIC18(7) [DiR] is a lipophilic, near-infrared fluorescent cyanine dye. The dye is useful for labeling cytoplasmic membranes and has been widely used for near-infrared in vivo imaging and for in vitro studies. The two long 18-carbon chains insert into the cell membrane, resulting in specific and stable cell staining with no or minimal dye transfer between cells. DiR diffuses laterally to stain the entire cell, allowing it to be used as an anterograde and retrograde tracer of neurons. In intact tissue, the dye does not transfer from labeled to unlabeled cells, but some transfer may occur when the membrane is disrupted, for example, after sectioning. Cell staining can be effected using the dye at 1-10 uM concentration and 10-20 min incubation time. The dye can be used for labeling nanoparticles and extracellular vescicles. The dye is weakly fluorescent until incorporated into membranes. Spectral Data: Excitation max: ~ 748nm, Emission max: ~780nm (MeOH).
Smiles: CC1(C)C2=C(C=CC=C2)N(CCCCCCCCCCCCCCCCCC)C1=CC=CC=CC=CC3=[N+](CCCCCCCCCCCCCCCCCC)C4=C(C3(C)C)C=CC=C4.[I-]
InChi Key: JLIOTPLALDYAEH-UHFFFAOYSA-M
References: (1) V. Kalchenko, et al., J. Biomed. Opt. 11, 050507 (2006) , (2) M. Eisenblatter, et al., J. Nucl. Med. 50, 1676 (2009) , (3) A. Wagh, et al., ioconjug. Chem. 23, 981 (2012) , (4) H. Liu & D. Wu, Curr. Drug Deliv. 13, 40 (2016) , (5) M.T. Berninger, et al., Eur. Radiol. 27, 1105 (2017) , (6) X. Zhang, et al., ACS Appl. Mater. Interfaces 10, 30155 (2018) , (7) M. Riehl, et al., Eur. J. Pharm. Sci. 132, 27 (2019) , (8) M. Bhogal, et al., Sci. Rep. 12, 6338 (2022) , (9) J. Santelices, et al., Bio. Protoc. 12, e4447 (2022)
InChi: InChI=1S/C63H101N2.HI/c1-7-9-11-13-15-17-19-21-23-25-27-29-31-33-38-46-54-64-58-50-44-42-48-56(58)62(3,4)60(64)52-40-36-35-37-41-53-61-63(5,6)57-49-43-45-51-59(57)65(61)55-47-39-34-32-30-28-26-24-22-20-18-16-14-12-10-8-2,/h35-37,40-45,48-53H,7-34,38-39,46-47,54-55H2,1-6H3,1H/q+1,/p-1

DiIC18(7) [DiR] is a lipophilic, near-infrared fluorescent cyanine dye. The dye is useful for labeling cytoplasmic membranes and has been widely used for near-infrared in vivo imaging and for in vitro studies. The two long 18-carbon chains insert into the cell membrane, resulting in specific and stable cell staining with no or minimal dye transfer between cells. DiR diffuses laterally to stain the entire cell, allowing it to be used as an anterograde and retrograde tracer of neurons. In intact tissue, the dye does not transfer from labeled to unlabeled cells, but some transfer may occur when the membrane is disrupted, for example, after sectioning. Cell staining can be effected using the dye at 1-10 uM concentration and 10-20 min incubation time. The dye can be used for labeling nanoparticles and extracellular vescicles. The dye is weakly fluorescent until incorporated into membranes. Spectral Data: Excitation max: ~ 748nm, Emission max: ~780nm (MeOH).

SKU: D1020 Category: Fluorescent Detection

Additional information

Additional information

Synonyms	DiIC18(7), DiR, Cy7 DiC18
Purity	≥97% (HPLC)
Appearance	Dark purple solid
CAS-Number	100068-60-8
Molecular Formula	C63H101N2.I
Molecular Weight	1013.40
Identity	1H-NMR
Solvents	DMSO or methanol
Smiles	CC1(C)C2=C(C=CC=C2)N(CCCCCCCCCCCCCCCCCC)C1=CC=CC=CC=CC3=[N+](CCCCCCCCCCCCCCCCCC)C4=C(C3(C)C)C=CC=C4.[I-]
InChi Key	JLIOTPLALDYAEH-UHFFFAOYSA-M
Shipping	AMBIENT
Short Term Storage	+4°C
Long Term Storage	-20°C
Handling Advice	Protect from light and air. Store under inert gas.
Use / Stability	Stable for at least 2 years after receipt when stored at -20°C.
Hazard statements	H315-H319-H335
Precautionary statements	P261-P264-P271-P280-P302+P352-P304+P340-P305+P351+P338-P312-P362-P403+P233-P501
GHS Symbol	GHS07
Signal word	Warning
Transportation	Not dangerous goods
References	(1) V. Kalchenko, et al., J. Biomed. Opt. 11, 050507 (2006), (2) M. Eisenblatter, et al., J. Nucl. Med. 50, 1676 (2009), (3) A. Wagh, et al., ioconjug. Chem. 23, 981 (2012), (4) H. Liu & D. Wu, Curr. Drug Deliv. 13, 40 (2016), (5) M.T. Berninger, et al., Eur. Radiol. 27, 1105 (2017), (6) X. Zhang, et al., ACS Appl. Mater. Interfaces 10, 30155 (2018), (7) M. Riehl, et al., Eur. J. Pharm. Sci. 132, 27 (2019), (8) M. Bhogal, et al., Sci. Rep. 12, 6338 (2022), (9) J. Santelices, et al., Bio. Protoc. 12, e4447 (2022)
InChi	InChI=1S/C63H101N2.HI/c1-7-9-11-13-15-17-19-21-23-25-27-29-31-33-38-46-54-64-58-50-44-42-48-56(58)62(3,4)60(64)52-40-36-35-37-41-53-61-63(5,6)57-49-43-45-51-59(57)65(61)55-47-39-34-32-30-28-26-24-22-20-18-16-14-12-10-8-2,/h35-37,40-45,48-53H,7-34,38-39,46-47,54-55H2,1-6H3,1H/q+1,/p-1
Quantity	50 mg, 100 mg, 250 mg, Bulk