2′-(4-Methylumbelliferyl)-a-D-N-acetylneuraminic acid sodium salt hydrate

SKU: M0096

Category: Fluorescent Detection

Synonyms: 4-MUNANA , 4-Methylumbelliferyl-N-acetyl-α-D-neuraminic acid sodium salt hydrate , Neu5Ac-α-4MU , 4MU-NeuNAc; 76204-02-9 (anhydr
CAS-Number: C₂₁H₂₄NNaO₁₁ . xH₂O
Molecular Formula: 489.41 (anhydrous basis)
Molecular Weight

Product Data
Handling
Safety Information
Details

Specifications

Purity: ≥95% (NMR)
Identity: 1H-NMR
Appearance: Light yellow powder

Properties

Fluorescence: λex 315 nm, λem 374 nm (pH 7.0) , λex 365 nm, λem 445 nm (after cleavage by neuraminidase)
Melting Point: 156-159 °C
Solvents: water, methanol

Downloads

Safety Data Sheet: CDX M0096 MSDS.pdf

Shipping: AMBIENT
Use / Stability: Stable for at least 2 years after receipt when stored at -20°C.
Handling Advice: Protect from light and moisture.
Long Term Storage: -20°C
Short Term Storage: +4°C

Transportation: Not dangerous goods

Description: Fluorogenic substrate used to measure sensitively enzymatic neuraminidase activity in fluorometric assays in mammalian and viral sources. Neuraminidase is catalyzed by the hydrolysation to form N-acetylneuraminic acid and the blue fluorescent soluble compound 4-Methylumbelliferone, which can be detected fluorometrically. Spectral data: ?ex 315 nm| ?em 374 nm (pH.7.0) or ?ex 365 nm| ?em 445 nm (after cleavage by neuraminidase). Used for fluorescent staining of sialidases in PAGE.
InChi: InChI=1S/C21H25NO11.Na.H2O/c1-9-5-16(27)31-15-6-11(3-4-12(9)15)32-21(20(29)30)7-13(25)17(22-10(2)24)19(33-21)18(28)14(26)8-23
References: (1) M. Potier M, et al.: Anal. Biochem. 94, 287 (1979) , (2) R.W. Myers, et al.: Anal. Biochem. 101, 166 (1980) , (3) S. Matsuzaki, et al.: Jpn. J. Med. 23, 123 (1987) , (4) E. Monti, et al.: Glycobiol. 9, 1313 (1999) , (5) N.T. Wetherall, et al.: J. Clin. Microbiol. 41, 742 (2003) , (6) D.P. Nayak & U. Reichl , J. Virol. Meth. 122, 9 (2004) , (7) H.L. Yen, et al.: J. Virol. 80, 8787 (2006) , (8) H. Zhi, et al.: J. Spectrosc. 2013, 1 (2014)
InChi Key: NSQMRVBWXQQIKF-HCNBQXOJSA-M
Smiles: O[C@@H]1[C@@H](NC(C)=O)[C@H]([C@H](O)[C@H](O)CO)O[C@@](OC2=CC(OC(C=C3C)=O)=C3C=C2)(C(O[Na])=O)C1.O

Fluorogenic substrate used to measure sensitively enzymatic neuraminidase activity in fluorometric assays in mammalian and viral sources. Neuraminidase is catalyzed by the hydrolysation to form N-acetylneuraminic acid and the blue fluorescent soluble compound 4-Methylumbelliferone, which can be detected fluorometrically. Spectral data: ?ex 315 nm| ?em 374 nm (pH.7.0) or ?ex 365 nm| ?em 445 nm (after cleavage by neuraminidase). Used for fluorescent staining of sialidases in PAGE.

SKU: M0096 Category: Fluorescent Detection

Additional information

Additional information

Synonyms	4-MUNANA, 4-Methylumbelliferyl-N-acetyl-α-D-neuraminic acid sodium salt hydrate, Neu5Ac-α-4MU, 4MU-NeuNAc
Purity	≥95% (NMR)
Appearance	Light yellow powder
CAS-Number	76204-02-9 (anhydr
Molecular Formula	C21H24NNaO11 . xH2O
Molecular Weight	489.41 (anhydrous basis)
Identity	1H-NMR
Solvents	water, methanol
Melting Point	156-159 °C
Fluorescence	λex 315 nm, λem 374 nm (pH 7.0), λex 365 nm, λem 445 nm (after cleavage by neuraminidase)
Smiles	O[C@@H]1[C@@H](NC(C)=O)[C@H]([C@H](O)[C@H](O)CO)O[C@@](OC2=CC(OC(C=C3C)=O)=C3C=C2)(C(O[Na])=O)C1.O
InChi Key	NSQMRVBWXQQIKF-HCNBQXOJSA-M
Shipping	AMBIENT
Short Term Storage	+4°C
Long Term Storage	-20°C
Handling Advice	Protect from light and moisture.
Use / Stability	Stable for at least 2 years after receipt when stored at -20°C.
Transportation	Not dangerous goods
References	(1) M. Potier M, et al.: Anal. Biochem. 94, 287 (1979), (2) R.W. Myers, et al.: Anal. Biochem. 101, 166 (1980), (3) S. Matsuzaki, et al.: Jpn. J. Med. 23, 123 (1987), (4) E. Monti, et al.: Glycobiol. 9, 1313 (1999), (5) N.T. Wetherall, et al.: J. Clin. Microbiol. 41, 742 (2003), (6) D.P. Nayak & U. Reichl, J. Virol. Meth. 122, 9 (2004), (7) H.L. Yen, et al.: J. Virol. 80, 8787 (2006), (8) H. Zhi, et al.: J. Spectrosc. 2013, 1 (2014)
InChi	InChI=1S/C21H25NO11.Na.H2O/c1-9-5-16(27)31-15-6-11(3-4-12(9)15)32-21(20(29)30)7-13(25)17(22-10(2)24)19(33-21)18(28)14(26)8-23
Quantity	1 mg, 5 mg, 25 mg, Bulk