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      2′,7′-Dichlorofluorescein

      SKU
      D0004

      Categories: Fluorescent Detection, Fluorescent Detection

      Synonyms
      Dichlorofluorescein , DCF
      76-54-0
      CAS-Number
      C20H10Cl2O5
      Molecular Formula
      401.2
      Molecular Weight

      For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

      • Product Data
      • Handling
      • Safety Information
      • Details

      Specifications

      Purity
      ≥90% (T)
      Appearance
      Beige powder
      Identity
      1H-NMR

      Properties

      Solvents
      ethanol (25 mg/ml), water (01mg/ml), DMSO
      Melting Point
      280 °C (dec.)(lit.)
      Fluorescence
      λex 504 nm , λem 529 nm in 0.1 M Tris pH 8.0

      Documentation

      Safety Data Sheet (SDS)
      CDX D0004 MSDS.pdf
      Shipping
      AMBIENT
      Short Term Storage
      +4°C
      Long Term Storage
      +4°C
      Handling Advice
      Protect from light and moisture.
      Use / Stability
      Stable for at least 2 years after receipt when stored at +4°C.
      Hazard statements
      H315-H319-H335
      Precautionary statements
      P261-P305 + P351 + P338
      GHS Symbol
      GHS07
      Signal word
      Warning
      Transportation
      Not dangerous goods
      Description
      Fluorescent dye. Spectral data: Extinction 504nm/Emmission 529nm, in 0.1 M Tris pH 8.0. Used for the determination of carbohydrates by fluorescence densitometry after TLC, in the determination of H2O2 by chemiluminescence, and as a fluorescent additive used in indirect fluorimetric detection in OTC-HPLC. Used as a component/substrate of the 2?,7?-dichlorofluorescein diacetate (DCFH-DA) assay to quantitate reactive oxygen species (ROS).
      Smiles
      O=C(C1=C2C=CC=C1)OC32C4=C(C=C(O)C(Cl)=C4)OC5=CC(O)=C(Cl)C=C53
      InChi Key
      VFNKZQNIXUFLBC-UHFFFAOYSA-N
      References
      (1) M.L. Baumann & A.L. Hatmaker , Clin. Chem. 7, 356 (1961) , (2) G. Gübitz, et al.: J. Chromatogr. 117, 337, (1976) , (3) H. Watanabe, et al.: Anal. Sci. 2, 461, (1986) , (4) W.D. Pfeffer & E.S. Yeung , J. Chromatogr. 506, 401, (1990) , (5) H. Zhu, et al.: Arch. Toxicol. 68, 582 (1994)
      InChi
      InChI=1S/C20H10Cl2O5/c21-13-5-11-17(7-15(13)23)26-18-8-16(24)14(22)6-12(18)20(11)10-4-2-1-3-9(10)19(25)27-20/h1-8,23-24H

      Fluorescent dye. Spectral data: Extinction 504nm/Emmission 529nm, in 0.1 M Tris pH 8.0. Used for the determination of carbohydrates by fluorescence densitometry after TLC, in the determination of H2O2 by chemiluminescence, and as a fluorescent additive used in indirect fluorimetric detection in OTC-HPLC. Used as a component/substrate of the 2?,7?-dichlorofluorescein diacetate (DCFH-DA) assay to quantitate reactive oxygen species (ROS).

      SKU: D0004 Categories: Fluorescent Detection, Fluorescent Detection
      • Additional information

      Additional information

      Synonyms

      Dichlorofluorescein, DCF

      Purity

      ≥90% (T)

      Appearance

      Beige powder

      CAS-Number

      76-54-0

      Molecular Formula

      C20H10Cl2O5

      Molecular Weight

      401.2

      Identity

      1H-NMR

      Solvents

      ethanol (25 mg/ml), water (01mg/ml), DMSO

      Melting Point

      280 °C (dec.)(lit.)

      Fluorescence

      λex 504 nm, λem 529 nm in 0.1 M Tris pH 8.0

      Smiles

      O=C(C1=C2C=CC=C1)OC32C4=C(C=C(O)C(Cl)=C4)OC5=CC(O)=C(Cl)C=C53

      InChi Key

      VFNKZQNIXUFLBC-UHFFFAOYSA-N

      Shipping

      AMBIENT

      Short Term Storage

      +4°C

      Long Term Storage

      +4°C

      Handling Advice

      Protect from light and moisture.

      Use / Stability

      Stable for at least 2 years after receipt when stored at +4°C.

      Hazard statements

      H315-H319-H335

      Precautionary statements

      P261-P305 + P351 + P338

      GHS Symbol

      GHS07

      Signal word

      Warning

      Transportation

      Not dangerous goods

      References

      (1) M.L. Baumann & A.L. Hatmaker, Clin. Chem. 7, 356 (1961), (2) G. Gübitz, et al.: J. Chromatogr. 117, 337, (1976), (3) H. Watanabe, et al.: Anal. Sci. 2, 461, (1986), (4) W.D. Pfeffer & E.S. Yeung, J. Chromatogr. 506, 401, (1990), (5) H. Zhu, et al.: Arch. Toxicol. 68, 582 (1994)

      InChi

      InChI=1S/C20H10Cl2O5/c21-13-5-11-17(7-15(13)23)26-18-8-16(24)14(22)6-12(18)20(11)10-4-2-1-3-9(10)19(25)27-20/h1-8,23-24H

      Quantity

      10 g, 25 g, 100 g, Bulk

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