Available from stock
Categories: Fluorescent Detection, Fluorescent Detection
Fluorescent dye. Spectral data: Extinction 504nm/Emmission 529nm, in 0.1 M Tris pH 8.0. Used for the determination of carbohydrates by fluorescence densitometry after TLC, in the determination of H2O2 by chemiluminescence, and as a fluorescent additive used in indirect fluorimetric detection in OTC-HPLC. Used as a component/substrate of the 2?,7?-dichlorofluorescein diacetate (DCFH-DA) assay to quantitate reactive oxygen species (ROS).
| Synonyms | Dichlorofluorescein, DCF |
|---|---|
| Purity | ≥90% (T) |
| Appearance | Beige powder |
| CAS-Number | 76-54-0 |
| Molecular Formula | C20H10Cl2O5 |
| Molecular Weight | 401.2 |
| Identity | 1H-NMR |
| Solvents | ethanol (25 mg/ml), water (01mg/ml), DMSO |
| Melting Point | 280 °C (dec.)(lit.) |
| Fluorescence | λex 504 nm, λem 529 nm in 0.1 M Tris pH 8.0 |
| Smiles | O=C(C1=C2C=CC=C1)OC32C4=C(C=C(O)C(Cl)=C4)OC5=CC(O)=C(Cl)C=C53 |
| InChi Key | VFNKZQNIXUFLBC-UHFFFAOYSA-N |
| Shipping | AMBIENT |
| Short Term Storage | +4°C |
| Long Term Storage | +4°C |
| Handling Advice | Protect from light and moisture. |
| Use / Stability | Stable for at least 2 years after receipt when stored at +4°C. |
| Hazard statements | H315-H319-H335 |
| Precautionary statements | P261-P305 + P351 + P338 |
| GHS Symbol | GHS07 |
| Signal word | Warning |
| Transportation | Not dangerous goods |
| References | (1) M.L. Baumann & A.L. Hatmaker, Clin. Chem. 7, 356 (1961), (2) G. Gübitz, et al.: J. Chromatogr. 117, 337, (1976), (3) H. Watanabe, et al.: Anal. Sci. 2, 461, (1986), (4) W.D. Pfeffer & E.S. Yeung, J. Chromatogr. 506, 401, (1990), (5) H. Zhu, et al.: Arch. Toxicol. 68, 582 (1994) |
| InChi | InChI=1S/C20H10Cl2O5/c21-13-5-11-17(7-15(13)23)26-18-8-16(24)14(22)6-12(18)20(11)10-4-2-1-3-9(10)19(25)27-20/h1-8,23-24H |
| Quantity | 10 g, 25 g, 100 g, Bulk |
4-(N,N-Diethylaminomethyl)-7-methoxy-coumarin 
