Available from stock

2′,7′-Dichlorofluorescein

SKU: D0004

Category: Fluorescent Detection

Synonyms: Dichlorofluorescein , DCF; 76-54-0
CAS-Number: C₂₀H₁₀Cl₂O₅
Molecular Formula: 401.2
Molecular Weight

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Product Data
Handling
Safety Information
Details

Specifications

Purity: ≥90% (Titr.)
Appearance: Beige powder
Identity: 1H-NMR

Properties

Solvents: ethanol (25 mg/ml), water (01mg/ml), DMSO
Melting Point: 280 °C (dec.)(lit.)
Density: 0.790 g/cm3
Fluorescence: λex 504 nm , λem 529 nm in 0.1 M Tris pH 8.0

Documentation

Safety Data Sheet (SDS): CDX D0004 MSDS.pdf

Shipping: AMBIENT
Short Term Storage: +4°C
Long Term Storage: +4°C
Handling Advice: Protect from light and moisture.
Use / Stability: Stable for at least 2 years after receipt when stored at +4°C.

Transportation: Not dangerous goods

Description: Fluorescent dye. Spectral data: Extinction 504nm/Emmission 529nm, in 0.1 M Tris pH 8.0. Used for the determination of carbohydrates by fluorescence densitometry after TLC, in the determination of H2O2 by chemiluminescence, and as a fluorescent additive used in indirect fluorimetric detection in OTC-HPLC. Used as a component/substrate of the 2?,7?-dichlorofluorescein diacetate (DCFH-DA) assay to quantitate reactive oxygen species (ROS).
Smiles: O=C(C1=C2C=CC=C1)OC32C4=C(C=C(O)C(Cl)=C4)OC5=CC(O)=C(Cl)C=C53
InChi Key: VFNKZQNIXUFLBC-UHFFFAOYSA-N
References: (1) M.L. Baumann & A.L. Hatmaker , Clin. Chem. 7, 356 (1961) , (2) G. Gübitz, et al.: J. Chromatogr. 117, 337, (1976) , (3) H. Watanabe, et al.: Anal. Sci. 2, 461, (1986) , (4) W.D. Pfeffer & E.S. Yeung , J. Chromatogr. 506, 401, (1990) , (5) H. Zhu, et al.: Arch. Toxicol. 68, 582 (1994)
InChi: InChI=1S/C20H10Cl2O5/c21-13-5-11-17(7-15(13)23)26-18-8-16(24)14(22)6-12(18)20(11)10-4-2-1-3-9(10)19(25)27-20/h1-8,23-24H

Fluorescent dye. Spectral data: Extinction 504nm/Emmission 529nm, in 0.1 M Tris pH 8.0. Used for the determination of carbohydrates by fluorescence densitometry after TLC, in the determination of H2O2 by chemiluminescence, and as a fluorescent additive used in indirect fluorimetric detection in OTC-HPLC. Used as a component/substrate of the 2?,7?-dichlorofluorescein diacetate (DCFH-DA) assay to quantitate reactive oxygen species (ROS).

SKU: D0004 Category: Fluorescent Detection

Additional information

Additional information

Synonyms	Dichlorofluorescein, DCF
Purity	≥90% (Titr.)
Appearance	Beige powder
CAS-Number	76-54-0
Molecular Formula	C20H10Cl2O5
Molecular Weight	401.2
Identity	1H-NMR
Solvents	ethanol (25 mg/ml), water (01mg/ml), DMSO
Melting Point	280 °C (dec.)(lit.)
Density	0.790 g/cm3
Fluorescence	λex 504 nm, λem 529 nm in 0.1 M Tris pH 8.0
Smiles	O=C(C1=C2C=CC=C1)OC32C4=C(C=C(O)C(Cl)=C4)OC5=CC(O)=C(Cl)C=C53
InChi Key	VFNKZQNIXUFLBC-UHFFFAOYSA-N
Shipping	AMBIENT
Short Term Storage	+4°C
Long Term Storage	+4°C
Handling Advice	Protect from light and moisture.
Use / Stability	Stable for at least 2 years after receipt when stored at +4°C.
Transportation	Not dangerous goods
References	(1) M.L. Baumann & A.L. Hatmaker, Clin. Chem. 7, 356 (1961), (2) G. Gübitz, et al.: J. Chromatogr. 117, 337, (1976), (3) H. Watanabe, et al.: Anal. Sci. 2, 461, (1986), (4) W.D. Pfeffer & E.S. Yeung, J. Chromatogr. 506, 401, (1990), (5) H. Zhu, et al.: Arch. Toxicol. 68, 582 (1994)
InChi	InChI=1S/C20H10Cl2O5/c21-13-5-11-17(7-15(13)23)26-18-8-16(24)14(22)6-12(18)20(11)10-4-2-1-3-9(10)19(25)27-20/h1-8,23-24H
Quantity	10 g, 25 g, 100 g, Bulk