Available from stock
Categories: Fluorescent Detection, Fluorescent Detection
3-(2-Furoyl)quinoline-2-carboxaldehyde (FQCA) is a fluorogenic derivatizing reagent for liquid chromatography suitable for high-sensitivity analysis of amino acids by laser-induced fluorescence with an argon-ion laser. The reagent forms highly fluorescent isoindoles upon reaction with primary amines. It is used for the determination of isoelectronic points (pI values) of proteins with laser-induced fluorescence detection (c-IEF-LIF). C-IEF-LIF technique can routinely detect 10-11 M of FQCA-labeled protein, compared with 10-7 M by traditional slab gel IEF and silver staining.
| Synonyms | FQ |
|---|---|
| Purity | ≥95% (HPLC) |
| Appearance | White to off-white powder |
| CAS-Number | 126769-01-5 |
| Molecular Formula | C15H9NO3 |
| Molecular Weight | 251.24 |
| Identity | 1H-NMR |
| Solvents | methanol |
| Fluorescence | λex 486 nm, λem ~600 nm in 0.1 M sodium borate pH 9.0 (after derivatization with glycine) |
| Smiles | [H]C(=O)C1=NC2=CC=CC=C2C=C1C(=O)C1=CC=CO1 |
| InChi Key | PNCHURHVMDRFTR-UHFFFAOYSA-N |
| Shipping | AMBIENT |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice | Protect from light and moisture. |
| Use / Stability | Stable for at least 2 years after receipt when stored at -20°C. |
| Hazard statements | H302, H315, H319, H335 |
| Precautionary statements | P261, P305 + P351 + P338 |
| GHS Symbol | GHS07 |
| Signal word | Warning |
| Transportation | Not dangerous goods |
| References | (1) D. Pinto et al.: J. Chrom. B 793(1), 107 (2003), (2) D.P. Richards et al.: J. Chromatog. A. 853, 21 (1999), (3) I.H. Lee et al.: Anal. Chem. 70, 4546 (1998), (4) D.B. Craig et al.: Electrophoresis 19, 2175 (1998), (5) S.C. Beale et al.: J. Chromatog. 499, 579 (1990) |
| InChi | InChI=1S/C15H9NO3/c17-9-13-11(15(18)14-6-3-7-19-14)8-10-4-1-2-5-12(10)16-13/h1-9H |
| Quantity | 10 mg, 50 mg, Bulk |
5-FITC DA 
