Available from stock

3-Carboxy-6,8-difluoro-7-hydroxycoumarin N-succinimidyl ester

SKU: C0036

Category: Fluorescent Detection

Synonyms: NHS Pacific Blue succinimidyl ester , 2,5-Dioxopyrrolidin-1-yl 6,8-difluoro-7-hydroxy-2-oxo-2H-chromene-3-carboxylate; 215868-33-0
CAS-Number: C₁₄H₇F₂NO₇
Molecular Formula: 339.2
Molecular Weight

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Product Data
Handling
Safety Information
Details

Specifications

Purity: ≥98% (HPLC)
Appearance: Yellow powder
Identity: 1H-NMR
Fluorescence: 451±4 nm in buffer pH 9

Properties

Solvents: chloroform, DMSO
UV / Vis: 410 nm in methanol
Fluorescence: 450 nm in methanol

Documentation

Safety Data Sheet (SDS): CDX C0036 MSDS.pdf

Shipping: AMBIENT
Short Term Storage: +4°C
Long Term Storage: -20°C
Handling Advice: Protect from light and moisture.
Use / Stability: Stable for at least 2 years after receipt when stored at -20°C.

Hazard statements: H315-H319-H335
Precautionary statements: P261-P305 + P351 + P338
GHS Symbol: GHS07
Signal word: Warning
Transportation: Not dangerous goods

Description: This amine-reactive reagent can be used to create blue-fluorescent bioconjugates with excitation/emission maxima ~410/455 nm that are excitable by the 405 nm spectral line of the blue diode (violet) laser. Used in highly sensitive analyses of amines, amino acids and dipeptides. Amine-reactive reagents react with non-protonated aliphatic amine groups, including the amine terminus of proteins and the ?-amino group of lysines.
Smiles: OC1=C(F)C2=C(C=C1F)C=C(C(=O)ON1C(=O)CCC1=O)C(=O)O2
InChi Key: NZYXABPVYJRICY-UHFFFAOYSA-N
References: (1) B. Abrams, et al.: Anal. Biochem. 386, 262 (2009) , (2) T.N. Chiesl, et al.: Anal. Chem. 81, 2537 (2009) , (3) J.D. Cohen, et al.: Biochem. 50, 8221 (2011) , (4) A.M. Stockton, et al.: Dyes & Pigments 96, 148 (2013)
InChi: InChI=1S/C14H7F2NO7/c15-7-4-5-3-6(13(21)23-12(5)10(16)11(7)20)14(22)24-17-8(18)1-2-9(17)19/h3-4,20H,1-2H2

This amine-reactive reagent can be used to create blue-fluorescent bioconjugates with excitation/emission maxima ~410/455 nm that are excitable by the 405 nm spectral line of the blue diode (violet) laser. Used in highly sensitive analyses of amines, amino acids and dipeptides. Amine-reactive reagents react with non-protonated aliphatic amine groups, including the amine terminus of proteins and the ?-amino group of lysines.

SKU: C0036 Category: Fluorescent Detection

Additional information

Additional information

Synonyms	NHS Pacific Blue succinimidyl ester, 2,5-Dioxopyrrolidin-1-yl 6,8-difluoro-7-hydroxy-2-oxo-2H-chromene-3-carboxylate
Purity	≥98% (HPLC)
Appearance	Yellow powder
CAS-Number	215868-33-0
Molecular Formula	C14H7F2NO7
Molecular Weight	339.2
Identity	1H-NMR
Solvents	chloroform, DMSO
UV / Vis	410 nm in methanol
Fluorescence	450 nm in methanol
Smiles	OC1=C(F)C2=C(C=C1F)C=C(C(=O)ON1C(=O)CCC1=O)C(=O)O2
InChi Key	NZYXABPVYJRICY-UHFFFAOYSA-N
Shipping	AMBIENT
Short Term Storage	+4°C
Long Term Storage	-20°C
Handling Advice	Protect from light and moisture.
Use / Stability	Stable for at least 2 years after receipt when stored at -20°C.
Hazard statements	H315-H319-H335
Precautionary statements	P261-P305 + P351 + P338
GHS Symbol	GHS07
Signal word	Warning
Transportation	Not dangerous goods
References	(1) B. Abrams, et al.: Anal. Biochem. 386, 262 (2009), (2) T.N. Chiesl, et al.: Anal. Chem. 81, 2537 (2009), (3) J.D. Cohen, et al.: Biochem. 50, 8221 (2011), (4) A.M. Stockton, et al.: Dyes & Pigments 96, 148 (2013)
InChi	InChI=1S/C14H7F2NO7/c15-7-4-5-3-6(13(21)23-12(5)10(16)11(7)20)14(22)24-17-8(18)1-2-9(17)19/h3-4,20H,1-2H2
Quantity	25 mg, 100 mg, Bulk
cdx-Absorbtion max.	410±3 nm in buffer pH 9
cdx-Fluorescence	451±4 nm in buffer pH 9