Available from stock

4-(4-Methoxybenzylamino)-7- nitro-2,1,3-benzoxadiazole

SKU: M0121

Category: Fluorescent Detection

Synonyms: 7-(p-Methoxybenzylamino)-4-nitrobenzoxadiazole, MBD, NSC 240872, NBD-Bz-p-Ome; 33984-50-8
CAS-Number: C₁₄H₁₂N₄O₄
Molecular Formula: 300.27
Molecular Weight

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Product Data
Handling
Safety Information
Details

Specifications

Purity: ≥98% (HPLC)
Appearance: Orange to red powder
Identity: 1H-NMR

Properties

Solvents: Soluble in DMSO (20mg/ml) or methanol.
Melting Point: 174-177° C
Boiling Point: 500.83° C at 760 mmHg (Predicted)
Refractive Index: n20D 1.69 (Predicted)
Density: 1.44 g/cm3 (Predicted)

Shipping: AMBIENT
Short Term Storage: +4°C
Long Term Storage: -20°C
Handling Advice: Very hygroscopic. Store under inert gas.
Use / Stability: Stable for at least 2 years after receipt when stored at -20°C.

Transportation: Not dangerous goods

Description: 4-(4-Methoxybenzylamino)-7-nitro-2,1,3-benzoxadiazole (MBD) is a compact fluorescent benzoxadiazole-based probe designed to selectively bind to hydrophobic (non-polar) regions of proteins and nucleoproteins. Upon binding to a hydrophobic region of a protein, MBD displays an emission of ~530nm upon excitation at ~430nm. Spectral Data: &lambda,Ex=~430nm, &lambda,Em=~530nm.
Smiles: O=[N+](C1=CC=C(NCC2=CC=C(OC)C=C2)C3=NON=C13)[O-]
InChi Key: IENONFJSMWUIQQ-UHFFFAOYSA-N
References: (1) R.A. Kenner & A.A. Aboderin, Biochemistry 10, 4433 (1971) , (2) Y. Kasahara, et al., FEBS Lett. 141, 128 (1982) , (3) T. Mizutani & A. Asaoka, Chem. Pharm. Bull. 32, 2395 (1984) , (4) S. Uchiyama, et al., Analyst 125, 1839 (2000) , (5) T. Oe, et al., Anal. Sci. 18, 161 (2002) , (6) A. Hellqvist, et al., Electrophoresis 34, 3252 (2013)
InChi: InChi=1S/C14H12N4O4/c1-21-10-4-2-9(3-5-10)8-15-11-6-7-12(18(19)20)14-13(11)16-22-17-14/h2-7,15H,8H2,1H3

4-(4-Methoxybenzylamino)-7-nitro-2,1,3-benzoxadiazole (MBD) is a compact fluorescent benzoxadiazole-based probe designed to selectively bind to hydrophobic (non-polar) regions of proteins and nucleoproteins. Upon binding to a hydrophobic region of a protein, MBD displays an emission of ~530nm upon excitation at ~430nm. Spectral Data: &lambda,Ex=~430nm, &lambda,Em=~530nm.

SKU: M0121 Category: Fluorescent Detection

Additional information

Additional information

Synonyms	7-(p-Methoxybenzylamino)-4-nitrobenzoxadiazole, MBD, NSC 240872, NBD-Bz-p-Ome
Purity	≥98% (HPLC)
Appearance	Orange to red powder
CAS-Number	33984-50-8
Molecular Formula	C14H12N4O4
Molecular Weight	300.27
Identity	1H-NMR
Solvents	Soluble in DMSO (20mg/ml) or methanol.
Melting Point	174-177° C
Boiling Point	500.83° C at 760 mmHg (Predicted)
Refractive Index	n20D 1.69 (Predicted)
Density	1.44 g/cm3 (Predicted)
Smiles	O=[N+](C1=CC=C(NCC2=CC=C(OC)C=C2)C3=NON=C13)[O-]
InChi Key	IENONFJSMWUIQQ-UHFFFAOYSA-N
Shipping	AMBIENT
Short Term Storage	+4°C
Long Term Storage	-20°C
Handling Advice	Very hygroscopic. Store under inert gas.
Use / Stability	Stable for at least 2 years after receipt when stored at -20°C.
Transportation	Not dangerous goods
References	(1) R.A. Kenner & A.A. Aboderin, Biochemistry 10, 4433 (1971), (2) Y. Kasahara, et al., FEBS Lett. 141, 128 (1982), (3) T. Mizutani & A. Asaoka, Chem. Pharm. Bull. 32, 2395 (1984), (4) S. Uchiyama, et al., Analyst 125, 1839 (2000), (5) T. Oe, et al., Anal. Sci. 18, 161 (2002), (6) A. Hellqvist, et al., Electrophoresis 34, 3252 (2013)
InChi	InChi=1S/C14H12N4O4/c1-21-10-4-2-9(3-5-10)8-15-11-6-7-12(18(19)20)14-13(11)16-22-17-14/h2-7,15H,8H2,1H3
Quantity	1 g, Bulk