Specifications
- Purity
- ≥97% (HPLC)
- Appearance
- Orange solid
- Identity
- 1H-NMR
Properties
- Solvents
- Soluble in DMSO.
- Fluorescence
- λex.498 nm, λem.517 nm
Available from stock
4′-(Aminomethyl)fluorescein hydrochlorid
- SKU
- A0075
Category: Fluorescent Detection
- Synonyms
- 4-AMF, 4'-((Aminoacetamido)methyl)fluorescein
- 91539-64-9
- CAS-Number
- C21H16ClNO5
- Molecular Formula
- 397.81
- Molecular Weight
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
- Shipping
- AMBIENT
- Short Term Storage
- -20°C
- Long Term Storage
- -20°C
- Handling Advice
- Protect from light and moisture.
- Use / Stability
- Stable for at least 2 years after receipt when stored at -20°C.
- Transportation
- Not dangerous goods
- Description
- 4'-(Aminomethyl)fluorescein is an excellent building block for developing fluoresceinated bioconjugates. Its amino group readily reacts with activated carboxy esters (such as NHS ester). The primary aliphatic amine of 4'-(aminomethyl)fluorescein can be reversibly coupled to aldehydes and ketones to form a Schiff base, which can be reduced to a stable amine derivative by sodium borohydride (NaBH4) or sodium cyanoborohydride (NaCNBH3) to form new probes. Carboxylic acids of proteins and other water-soluble biopolymers can be coupled to this molecule in aqueous solution using water-soluble carbodiimides such as EDAC. Spectral Data: λex 494 nm; λem 520 nm.
- Smiles
- OC1=CC=C(C(C2=CC=CC=C2C(O)=O)=C(C=C3)C(O4)=C(CN)C3=O)C4=C1.Cl
- InChi Key
- JJYRABHKUBHHSU-UHFFFAOYSA-N
- References
- (1) M.T. Shipchandler, et al.; Anal. Biochem. 162, 89 (1987) , (2) R. Dhar Dubey, et al.; Nanotheranostics 1, 59 (2017)
- InChi
- InChI=1S/C21H15NO5.ClH/c22-10-16-17(24)8-7-15-19(12-3-1-2-4-13(12)21(25)26)14-6-5-11(23)9-18(14)27-20(15)16;/h1-9,23H,10,22H2,(H,25,26);1H
4′-(Aminomethyl)fluorescein is an excellent building block for developing fluoresceinated bioconjugates. Its amino group readily reacts with activated carboxy esters (such as NHS ester). The primary aliphatic amine of 4′-(aminomethyl)fluorescein can be reversibly coupled to aldehydes and ketones to form a Schiff base, which can be reduced to a stable amine derivative by sodium borohydride (NaBH4) or sodium cyanoborohydride (NaCNBH3) to form new probes. Carboxylic acids of proteins and other water-soluble biopolymers can be coupled to this molecule in aqueous solution using water-soluble carbodiimides such as EDAC. Spectral Data: λex 494 nm; λem 520 nm.
Additional information
| Synonyms | 4-AMF, 4'-((Aminoacetamido)methyl)fluorescein |
|---|---|
| Purity | ≥97% (HPLC) |
| Appearance | Orange solid |
| CAS-Number | 91539-64-9 |
| Molecular Formula | C21H16ClNO5 |
| Molecular Weight | 397.81 |
| Identity | 1H-NMR |
| Solvents | Soluble in DMSO. |
| Fluorescence | λex.498 nm, λem.517 nm |
| Smiles | OC1=CC=C(C(C2=CC=CC=C2C(O)=O)=C(C=C3)C(O4)=C(CN)C3=O)C4=C1.Cl |
| InChi Key | JJYRABHKUBHHSU-UHFFFAOYSA-N |
| Shipping | AMBIENT |
| Short Term Storage | -20°C |
| Long Term Storage | -20°C |
| Handling Advice | Protect from light and moisture. |
| Use / Stability | Stable for at least 2 years after receipt when stored at -20°C. |
| Transportation | Not dangerous goods |
| References | (1) M.T. Shipchandler, et al.; Anal. Biochem. 162, 89 (1987), (2) R. Dhar Dubey, et al.; Nanotheranostics 1, 59 (2017) |
| InChi | InChI=1S/C21H15NO5.ClH/c22-10-16-17(24)8-7-15-19(12-3-1-2-4-13(12)21(25)26)14-6-5-11(23)9-18(14)27-20(15)16;/h1-9,23H,10,22H2,(H,25,26);1H |
| Quantity | 25 mg, 100 mg, Bulk |
