Available from stock

4-Methylumbelliferyl-beta-D-glucopyranosid

SKU: M0099

Category: Fluorescent Detection

Synonyms: MUD, 4-MU-&beta,-D-Glc, 4-Methylumbelliferyl-&beta,-D-glucoside; 18997-57-4
CAS-Number: C₁₆H₁₈O₈
Molecular Formula: 338.31
Molecular Weight

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Product Data
Handling
Safety Information
Details

Specifications

Purity: ≥97% (NMR)
Appearance: White to off-white powder
Identity: 1H-NMR

Properties

Solvents: Soluble in DMSO or DMF.
Melting Point: 210 °C
Density: 1.522 g/cm3

Shipping: AMBIENT
Short Term Storage: +4°C
Long Term Storage: -20°C
Handling Advice: Protect from light and moisture.
Use / Stability: Stable for at least 2 years after receipt when stored at -20°C.

Transportation: Not dangerous goods

Description: 4-Methylumbelliferyl-β-D-glucopyranoside (MUD) is a non-fluorescent but sensitive fluorogenic substrate for β-glucosidase and β-glucocerebrosidase (also known as glucosylceramidase) yielding a blue fluorescent solution. It is a fluorogenic MU substrate for detecting glucosidase in cell extracts and purified enzyme preparations using a fluorescence microplate reader or fluorometer. It is employed in the detection of β-glucosidase as an indicator of Enterococci. It has been extensively used in work on Gaucher′s disease, a lipid storage disease characterized by the accumulation of glucocerebroside due to a genetic deficiency of a β-glucosidase. The use of 4-Methylumbelliferyl-β-D-glucopyranoside has also been reported in a rapid method for identifying bacterial enzymes. Hydrolysis of MUD releases the fluorescent product 4-MU. Spectral Data. λEx/λEm= 360/450 nm (cleaved product, pH-dependent).
Smiles: O[C@H]1[C@H](OC2=CC=C(C(C)=CC(O3)=O)C3=C2)O[C@H](CO)[C@@H](O)[C@@H]1O
InChi Key: YUDPTGPSBJVHCN-YMILTQATSA-N
References: (1) S.P. Peters, et al.; Clin. Chim. Acta 60, 391 (1975) , (2) D.M. Broadhead & J. Butterworth; Clin. Chim. Acta 75, 155 (1977) , (3) A. Basu & R.H. Glew; J. Biol. Chem. 260, 13067 (1985) , (4) K.J. Panosian & S.C. Edberg; J. Clin. Microbiol. 27, 1719 (1989) , (5) S. Nakagawa, et al.; Clin. Chim. Acta. 118, 99 (1992) , (6) B. Setlow, et al.; J. Appl. Microbiol. 96, 1245 (2004) , (7) R. Kwapiszewski, et al.; Biomed. Microdevices 13, 431 (2011) , (8) M. Shanmuganathan & P. Britz-McKibbin; Anal. Bioanal. Chem. 399, 2843 (2011) , (9) L. Oftedal, et al.; Sci. Rep. 10, 22098 (2020)
InChi: InChI=1S/C16H18O8/c1-7-4-12(18)23-10-5-8(2-3-9(7)10)22-16-15(21)14(20)13(19)11(6-17)24-16/h2-5,11,13-17,19-21H,6H2,1H3/t11-,13-,14+,15-,16-/m1/s1

4-Methylumbelliferyl-β-D-glucopyranoside (MUD) is a non-fluorescent but sensitive fluorogenic substrate for β-glucosidase and β-glucocerebrosidase (also known as glucosylceramidase) yielding a blue fluorescent solution. It is a fluorogenic MU substrate for detecting glucosidase in cell extracts and purified enzyme preparations using a fluorescence microplate reader or fluorometer. It is employed in the detection of β-glucosidase as an indicator of Enterococci. It has been extensively used in work on Gaucher′s disease, a lipid storage disease characterized by the accumulation of glucocerebroside due to a genetic deficiency of a β-glucosidase. The use of 4-Methylumbelliferyl-β-D-glucopyranoside has also been reported in a rapid method for identifying bacterial enzymes. Hydrolysis of MUD releases the fluorescent product 4-MU. Spectral Data. λEx/λEm= 360/450 nm (cleaved product, pH-dependent).

SKU: M0099 Category: Fluorescent Detection

Additional information

Additional information

Synonyms	MUD, 4-MU-&beta,-D-Glc, 4-Methylumbelliferyl-&beta,-D-glucoside
Purity	≥97% (NMR)
Appearance	White to off-white powder
CAS-Number	18997-57-4
Molecular Formula	C16H18O8
Molecular Weight	338.31
Identity	1H-NMR
Solvents	Soluble in DMSO or DMF.
Melting Point	210 °C
Density	1.522 g/cm3
Smiles	O[C@H]1[C@H](OC2=CC=C(C(C)=CC(O3)=O)C3=C2)O[C@H](CO)[C@@H](O)[C@@H]1O
InChi Key	YUDPTGPSBJVHCN-YMILTQATSA-N
Shipping	AMBIENT
Short Term Storage	+4°C
Long Term Storage	-20°C
Handling Advice	Protect from light and moisture.
Use / Stability	Stable for at least 2 years after receipt when stored at -20°C.
Transportation	Not dangerous goods
References	(1) S.P. Peters, et al.; Clin. Chim. Acta 60, 391 (1975), (2) D.M. Broadhead & J. Butterworth; Clin. Chim. Acta 75, 155 (1977), (3) A. Basu & R.H. Glew; J. Biol. Chem. 260, 13067 (1985), (4) K.J. Panosian & S.C. Edberg; J. Clin. Microbiol. 27, 1719 (1989), (5) S. Nakagawa, et al.; Clin. Chim. Acta. 118, 99 (1992), (6) B. Setlow, et al.; J. Appl. Microbiol. 96, 1245 (2004), (7) R. Kwapiszewski, et al.; Biomed. Microdevices 13, 431 (2011), (8) M. Shanmuganathan & P. Britz-McKibbin; Anal. Bioanal. Chem. 399, 2843 (2011), (9) L. Oftedal, et al.; Sci. Rep. 10, 22098 (2020)
InChi	InChI=1S/C16H18O8/c1-7-4-12(18)23-10-5-8(2-3-9(7)10)22-16-15(21)14(20)13(19)11(6-17)24-16/h2-5,11,13-17,19-21H,6H2,1H3/t11-,13-,14+,15-,16-/m1/s1
Quantity	1 g, 5 g, Bulk