Available from stock

4-Methylumbelliferyl ß-D-galactopyranoside

SKU: M0225

Category: Fluorescent Detection

Synonyms: MUG, MUGA, MUGal, 4-Methylumbelliferyl-&beta,-D-galactoside; 6160-78-7
CAS-Number: C₁₆H₁₈O₈
Molecular Formula: 338.31
Molecular Weight

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Product Data
Handling
Safety Information
Details

Specifications

Purity: ≥98% (NMR)
Appearance: White to off-white powder
Identity: 1H-NMR

Properties

Solvents: Soluble in DMSO.
Melting Point: 260 °C - decomposes on heating

Shipping: AMBIENT
Short Term Storage: +4°C
Long Term Storage: -20°C
Handling Advice: Protect from light and moisture.
Use / Stability: Stable for at least 2 years after receipt when stored at -20°C.

Transportation: Not dangerous goods

Description: 4-Methylumbelliferyl-β-D-galactopyranoside (MUG) is a non-fluorescent but sensitive fluorogenic substrate for β-galactosidase (β-gal) yielding a blue fluorescent solution. It is a fluorogenic MU substrate for detecting galactosidase in cell extracts and purified enzyme preparations using a fluorescence microplate reader or fluorometer. It is used as a substrate in β-galactosidase activity assay. Hydrolysis of MUG releases the fluorescent product 4-MU. Spectral Data. λEx/λEm= 360/450 nm (cleaved product, pH-dependent).
Smiles: O[C@H]1[C@H](OC2=CC=C(C(C)=CC(O3)=O)C3=C2)O[C@H](CO)[C@H](O)[C@@H]1O
InChi Key: YUDPTGPSBJVHCN-DZQJYWQESA-N
References: (1) A.J. Furth & D. Robinson; Biochem. J. 97, 59 (1965) , (2) G. Kominami, et al.; Clin. Chim. Acta 103, 381 (1980) , (3) H. Labrousse, et al.; J. Immunol. Methods 48, 133 (1982) , (4) H. Ohmori, et al.; J. Immunol. Methods. 147, 119 (1992) , (5) J.D. Berg, et al.; Appl. Environ. Microbiol. 54, 2118 (1998) , (6) F. Vidal-Aroca, et al.; Biotechn. 40, 433 (2006)
InChi: InChI=1S/C16H18O8/c1-7-4-12(18)23-10-5-8(2-3-9(7)10)22-16-15(21)14(20)13(19)11(6-17)24-16/h2-5,11,13-17,19-21H,6H2,1H3/t11-,13+,14+,15-,16-/m1/s1

4-Methylumbelliferyl-β-D-galactopyranoside (MUG) is a non-fluorescent but sensitive fluorogenic substrate for β-galactosidase (β-gal) yielding a blue fluorescent solution. It is a fluorogenic MU substrate for detecting galactosidase in cell extracts and purified enzyme preparations using a fluorescence microplate reader or fluorometer. It is used as a substrate in β-galactosidase activity assay. Hydrolysis of MUG releases the fluorescent product 4-MU. Spectral Data. λEx/λEm= 360/450 nm (cleaved product, pH-dependent).

SKU: M0225 Category: Fluorescent Detection

Additional information

Additional information

Synonyms	MUG, MUGA, MUGal, 4-Methylumbelliferyl-&beta,-D-galactoside
Purity	≥98% (NMR)
Appearance	White to off-white powder
CAS-Number	6160-78-7
Molecular Formula	C16H18O8
Molecular Weight	338.31
Identity	1H-NMR
Solvents	Soluble in DMSO.
Melting Point	260 °C – decomposes on heating
Smiles	O[C@H]1[C@H](OC2=CC=C(C(C)=CC(O3)=O)C3=C2)O[C@H](CO)[C@H](O)[C@@H]1O
InChi Key	YUDPTGPSBJVHCN-DZQJYWQESA-N
Shipping	AMBIENT
Short Term Storage	+4°C
Long Term Storage	-20°C
Handling Advice	Protect from light and moisture.
Use / Stability	Stable for at least 2 years after receipt when stored at -20°C.
Transportation	Not dangerous goods
References	(1) A.J. Furth & D. Robinson; Biochem. J. 97, 59 (1965), (2) G. Kominami, et al.; Clin. Chim. Acta 103, 381 (1980), (3) H. Labrousse, et al.; J. Immunol. Methods 48, 133 (1982), (4) H. Ohmori, et al.; J. Immunol. Methods. 147, 119 (1992), (5) J.D. Berg, et al.; Appl. Environ. Microbiol. 54, 2118 (1998), (6) F. Vidal-Aroca, et al.; Biotechn. 40, 433 (2006)
InChi	InChI=1S/C16H18O8/c1-7-4-12(18)23-10-5-8(2-3-9(7)10)22-16-15(21)14(20)13(19)11(6-17)24-16/h2-5,11,13-17,19-21H,6H2,1H3/t11-,13+,14+,15-,16-/m1/s1
Quantity	1 g, 5 g, Bulk