Specifications
- Purity
- ≥97% (NMR)
- Appearance
- Off-white to yellow powder
- Identity
- 1H-NMR
Properties
- Solvents
- chloroform or DMSO.
Available from stock
5-Aminofluorescein diacetate
- SKU
- A0318
Categories: Fluorescent Detection, Fluorescent Detection
- Synonyms
- 5-AF DA
- 195977-46-9
- CAS-Number
- C24H17NO7
- Molecular Formula
- 431.39
- Molecular Weight
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
- Shipping
- AMBIENT
- Short Term Storage
- +4°C
- Long Term Storage
- -20°C
- Handling Advice
- Protect from light and moisture.
- Use / Stability
- Stable for at least 2 years after receipt when stored at -20°C.
- Hazard statements
- H315, H319, H335
- Precautionary statements
- P261, P305 + P351 + P338
- GHS Symbol
- GHS07
- Signal word
- Warning
- Transportation
- Not dangerous goods
- Description
- 5-Aminofluorescein diacetate is a non-fluorescent probe that after entering the cells can be cleaved by esterases to form 5-Aminofluorescein (5-AF) a fluorescence marker with excitation wavelength at 490 nm and the emission wavelength at 515 nm. The compound can be used as a negative control for the fluorescent probe DAF-2 DA. 5-Aminofluorescein diacetate could also be used as a starting material or intermediate in synthesis of fluorescent probes.
- Smiles
- NC1=CC=C(C2=C1)C3(OC2=O)C4=CC=C(OC(C)=O)C=C4OC5=CC(OC(C)=O)=CC=C53
- InChi Key
- OJIRUUMPJJZGSG-UHFFFAOYSA-N
- References
- (1) G.-S. Jiao, et al., J. Org. Chem. 68, 8264 (2003)
- InChi
- InChI=1S/C24H17NO7/c1-12(26)29-15-4-7-19-21(10-15)31-22-11-16(30-13(2)27)5-8-20(22)24(19)18-6-3-14(25)9-17(18)23(28)32-24/h3-11H,25H2,1-2H3
5-Aminofluorescein diacetate is a non-fluorescent probe that after entering the cells can be cleaved by esterases to form 5-Aminofluorescein (5-AF) a fluorescence marker with excitation wavelength at 490 nm and the emission wavelength at 515 nm. The compound can be used as a negative control for the fluorescent probe DAF-2 DA. 5-Aminofluorescein diacetate could also be used as a starting material or intermediate in synthesis of fluorescent probes.
Additional information
| Synonyms | 5-AF DA |
|---|---|
| Purity | ≥97% (NMR) |
| Appearance | Off-white to yellow powder |
| CAS-Number | 195977-46-9 |
| Molecular Formula | C24H17NO7 |
| Molecular Weight | 431.39 |
| Identity | 1H-NMR |
| Solvents | chloroform or DMSO. |
| Smiles | NC1=CC=C(C2=C1)C3(OC2=O)C4=CC=C(OC(C)=O)C=C4OC5=CC(OC(C)=O)=CC=C53 |
| InChi Key | OJIRUUMPJJZGSG-UHFFFAOYSA-N |
| Shipping | AMBIENT |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice | Protect from light and moisture. |
| Use / Stability | Stable for at least 2 years after receipt when stored at -20°C. |
| Hazard statements | H315, H319, H335 |
| Precautionary statements | P261, P305 + P351 + P338 |
| GHS Symbol | GHS07 |
| Signal word | Warning |
| Transportation | Not dangerous goods |
| References | (1) G.-S. Jiao, et al., J. Org. Chem. 68, 8264 (2003) |
| InChi | InChI=1S/C24H17NO7/c1-12(26)29-15-4-7-19-21(10-15)31-22-11-16(30-13(2)27)5-8-20(22)24(19)18-6-3-14(25)9-17(18)23(28)32-24/h3-11H,25H2,1-2H3 |
| Quantity | 250 mg, 1 g, 10 g, Bulk |
