Specifications
- Purity
- ≥99% (HPLC)
- Identity
- 1H-NMR
- Appearance
- White powder
Properties
- Melting Point
- 191-194 °C (lit.)
- Solvents
- DMSO (30 mg/ml), DMF (30 mg/ml) or ethanol (25 mg/ml) or water (10 mg/ml).
Category: Non Fluorescent Detection & Chromogene Detection
The synthetic nucleoside 5-Bromo-2′-deoxyuridine (BrdU) is a thymidine analog used to label DNA and commonly used in the detection of proliferating cells in living tissues. It can be incorporated into newly synthesized DNA in place of thymidine during the S phase of the cell cycle. Cells that were actively proliferating can then be detected by denaturing the DNA and allowing specific antibodies with fluorescent tags to target the BrdU incorporation for detection via flow cytometry or fluorescence microscopy. Binding of the antibody requires denaturation of the DNA, usually by exposing the cells to acid or heat. BrdU is also used as a mutagen in genetic research. BrdU stimulates cellular differentiation and maturation in leukemia cell lines, while it inhibits differentiation of friend erythroleukemia cells. BrdU replaces Oct-4 in transcription factor-mediated reprogramming of somatic cells and can be used in small molecule cocktail to generate ciPSCs.
Synonyms | 5-BrdU, 5-Bromo-1-(2-deoxy-&beta, -D-ribofuranosyl)uracil, 5-Bromouracil deoxyriboside, BUdR, Bromodeoxyuridine, Broxuridine, NSC 38297 |
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Purity | ≥99% (HPLC) |
Appearance | White powder |
CAS-Number | 59-14-3 |
Molecular Formula | C9H11BrN2O5 |
Molecular Weight | 307.10 |
Identity | 1H-NMR |
Solvents | DMSO (30 mg/ml), DMF (30 mg/ml) or ethanol (25 mg/ml) or water (10 mg/ml). |
Melting Point | 191-194 °C (lit.) |
Smiles | O[C@H]1C[C@H](N2C=C(Br)C(NC2=O)=O)O[C@@H]1CO |
InChi Key | WOVKYSAHUYNSMH-RRKCRQDMSA-N |
Shipping | AMBIENT |
Short Term Storage | +4°C |
Long Term Storage | -20°C |
Handling Advice | Protect from light and moisture. |
Use / Stability | Stable for at least 2 years after receipt when stored at -20°C. |
Hazard statements | H340-H361 |
Precautionary statements | P201-P281-P308 + P313 |
GHS Symbol | GHS08 |
Signal word | Danger |
Transportation | Not dangerous goods |
References | (1) N. Kee, et al., J. Neurosci. Methods 115, 97 (2002), (2) R.C. Leif, et al., Cytometry A 58, 45 (2004) (Review), (3) P. Taupin, Brain Res. Rev. 53, 198 (2007) (Review), (4) D.W. Stacey & M. Hitomi, Cytometry A 73, 270 (2008), (5) B.L. Cavanagh, et al., Molecules 16, 7980 (2011), (6) B. Lehner, et al., Cell Tissue Res. 345, 313 (2011), (7) T. Konishi, et al., J. Radiat. Res. 52, 433 (2011), (8) J.M. Barker, et al., PLoS One 8, e63692 (2013), (9) Y. Long, et al., Cell Res. 25, 1171 (2015) |
InChi | InChI=1S/C9H11BrN2O5/c10-4-2-12(9(16)11-8(4)15)7-1-5(14)6(3-13)17-7/h2,5-7,13-14H,1,3H2,(H,11,15,16)/t5-,6+,7+/m0/s1 |
Quantity | 1 g, 5 g, Bulk |