• Home
Results for {phrase} ({results_count} of {results_count_total})
Displaying {results_count} results of {results_count_total}

More results...

Filter by Product categories
Bioactive Molecules
Amino Acids, Peptides, Proteins
Carbohydrates
Lipids, Fatty Acids
Metabolites
Phytochemicals
Vitamins
Bioactive Small Molecules
Biopolymers
Detection in Bioanalysis
Fluorescent Detection
Organic Pollutants & Pesticides
Fungicides
Herbicides
Insecticides
Other Pollutants
Pharmaceutically Actives
Antibiotics
API's & Intermediates
Steroids
Reagents and Building Blocks
Reagents for Life Sciences
Buffers
Building Blocks, Intermediates, Reagents
Fluorescent Detection
Linker
Non Fluorescent Detection & Chromogene Detection
Quorum Sensing
Uncategorized
    Industriestrasse 15, CH-9015 St. Gallen, Tel: +41 71 244 48 25
    • 0Shopping Cart
    Chemodex
    • Home
    • Products
    • Catalogs
    • About Chemodex
    • Contact & Distributors
    • Menu Menu
    5-Carboxy-2'

    Available from stock

      Inquiry

      Please fill out the form for your inquiry:



      * required field

      5-Carboxy-2′,7′-dichlorofluorescein DA

      SKU
      C0333

      Categories: Fluorescent Detection, Fluorescent Detection

      Synonyms
      5-CDCFDA, 5-Carboxy-2',7'-dichlorofluorescein diacetate , 5-Carboxy-2',7'-dichlorofluorescein DA
      144489-09-8
      CAS-Number
      C25H14Cl2O9
      Molecular Formula
      529.28
      Molecular Weight

      For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

      • Product Data
      • Handling
      • Safety Information
      • Details

      Specifications

      Purity
      ≥95% (NMR)
      Appearance
      Off-white powder
      Identity
      1H-NMR

      Properties

      Solvents
      DMSO, DMF, acetonitrile, methanol, chloroform
      Shipping
      AMBIENT
      Short Term Storage
      +4°C
      Long Term Storage
      -20°C
      Handling Advice
      Protect from light and moisture.
      Use / Stability
      Stable for at least 2 years after receipt when stored at -20°C.
      Transportation
      Not dangerous goods
      Description
      Non-activated, amine-reactive fluorescent probe. Single 5-isomer of CDCFDA that can be used in biomolecules conjugation. CDCFDA is an amine–reactive fluorescein diacetate (FDA) derivative used to prepare a variety of FDA conjugates. CDCFDA is a non-fluorescent molecule that diffuse into cells and is hydrolyzed by intracellular non-specific esterases, yielding a fluorescent probe. The probe accumulates exclusively in viable cells with intact cell membranes. CFCFDA does not stain dead cells. CDCFDA labeling can be used for monitoring cells by flow cytometry or fluorescence microscopy. The probe is also frequently used to monitor reactive oxygen species (ROS) generation in live cells and intracellular pH levels. Spectral data: ?ex=504nm, ?em=529nm.
      Smiles
      ClC1=C(OC(C)=O)C=C2C(C3(C(C=CC(C(O)=O)=C4)=C4C(O3)=O)C(C=C(Cl)C(OC(C)=O)=C5)=C5O2)=C1
      InChi Key
      AJIVVDSJIOOZOJ-UHFFFAOYSA-N
      References
      (1) C.K. Raymond et al. , J. Cell Biol. 111, 877 (1990) , (2) J.P. Kehrer & T. Paraidathathu , Free Rad. Res. Commun. 16, 217 (1992) , (3) Q.P. Lloyd, et al. , J. Biol. Chem. 270, 22445 (1995) , (4) A.L. Inselman, et al. , Curr. Microbiol. 38, 295 (1999) , (5) M.J. Zamek-Gliszczynski, et al. , J. Pharmacol. Exp. Therap. 304, 801 (2003) , (6) L. Li, et al. , Free Radic. Res. 42, 354 (2008) , (7) M. Medhora, et al. , Am. J. Physiol. Lung Cell Mol. Physiol. 294, L902 (2008) , (8) S. Siissalo, et al. , Eur. J. Pharm. Biopharm. 71, 332 (2009) , (9) A.I. Pogue, et al. , Int. J. Mol. Sci. 13, 9615 (2012) , (10) J. Roh, et al. , Redox Biol. 11, 254 (2016) , (11) J.L. Roh, et al. , Cancer Lett. 381, 96 (2016)

      Non-activated, amine-reactive fluorescent probe. Single 5-isomer of CDCFDA that can be used in biomolecules conjugation. CDCFDA is an amine–reactive fluorescein diacetate (FDA) derivative used to prepare a variety of FDA conjugates. CDCFDA is a non-fluorescent molecule that diffuse into cells and is hydrolyzed by intracellular non-specific esterases, yielding a fluorescent probe. The probe accumulates exclusively in viable cells with intact cell membranes. CFCFDA does not stain dead cells. CDCFDA labeling can be used for monitoring cells by flow cytometry or fluorescence microscopy. The probe is also frequently used to monitor reactive oxygen species (ROS) generation in live cells and intracellular pH levels. Spectral data: ?ex=504nm, ?em=529nm.

      SKU: C0333 Categories: Fluorescent Detection, Fluorescent Detection
      • Additional information

      Additional information

      Synonyms

      5-CDCFDA, 5-Carboxy-2',7'-dichlorofluorescein diacetate, 5-Carboxy-2',7'-dichlorofluorescein DA

      Purity

      ≥95% (NMR)

      Appearance

      Off-white powder

      CAS-Number

      144489-09-8

      Molecular Formula

      C25H14Cl2O9

      Molecular Weight

      529.28

      Identity

      1H-NMR

      Solvents

      DMSO, DMF, acetonitrile, methanol, chloroform

      Smiles

      ClC1=C(OC(C)=O)C=C2C(C3(C(C=CC(C(O)=O)=C4)=C4C(O3)=O)C(C=C(Cl)C(OC(C)=O)=C5)=C5O2)=C1

      InChi Key

      AJIVVDSJIOOZOJ-UHFFFAOYSA-N

      Shipping

      AMBIENT

      Short Term Storage

      +4°C

      Long Term Storage

      -20°C

      Handling Advice

      Protect from light and moisture.

      Use / Stability

      Stable for at least 2 years after receipt when stored at -20°C.

      Transportation

      Not dangerous goods

      References

      (1) C.K. Raymond et al., J. Cell Biol. 111, 877 (1990), (2) J.P. Kehrer & T. Paraidathathu, Free Rad. Res. Commun. 16, 217 (1992), (3) Q.P. Lloyd, et al., J. Biol. Chem. 270, 22445 (1995), (4) A.L. Inselman, et al., Curr. Microbiol. 38, 295 (1999), (5) M.J. Zamek-Gliszczynski, et al., J. Pharmacol. Exp. Therap. 304, 801 (2003), (6) L. Li, et al., Free Radic. Res. 42, 354 (2008), (7) M. Medhora, et al., Am. J. Physiol. Lung Cell Mol. Physiol. 294, L902 (2008), (8) S. Siissalo, et al., Eur. J. Pharm. Biopharm. 71, 332 (2009), (9) A.I. Pogue, et al., Int. J. Mol. Sci. 13, 9615 (2012), (10) J. Roh, et al., Redox Biol. 11, 254 (2016), (11) J.L. Roh, et al., Cancer Lett. 381, 96 (2016)

      Quantity

      100 mg, 250 mg, Bulk

      Related products

      • Anthracene-9

        Anthracene-9,10-dipropionic acid disodium salt

        Purity: ≥95% (HPLC)CAS-Number: 82767-90-6SKU: A0116

        Read more Show Details
      • AMCA-H

        AMCA-H

        Purity: ≥90% (HPLC)CAS-Number: 106562-32-7SKU: A0009

        Read more Show Details
      • NBD-amine

        NBD-amine

        Purity: ≥99% (HPLC)CAS-Number: 10199-91-4SKU: A0176

        Read more Show Details
      • 6-(4-Acetamido-1

        6-(4-Acetamido-1,8-naphthalamido) hexanoic acid

        Purity: ≥95% (HPCE)CAS-Number: 172227-59-7SKU: A0038

        Read more Show Details
      © Copyright - Chemodex AG - Customized Molecules developed in Switzerland
      • Home
      • All products
      • Order and delivery information
      • Disclaimer
      • Privacy policy
      • About this site
      4-Chloromethylbenzonitrile4-ChloromethylbenzonitrileCoenzyme Q10Coenzyme Q10 Scroll to top