Available from stock

5-Carboxy-2′,7′-dichlorofluorescein DA

SKU: C0333

Categories: Fluorescent Detection, Fluorescent Detection

Synonyms: 5-CDCFDA, 5-Carboxy-2',7'-dichlorofluorescein diacetate , 5-Carboxy-2',7'-dichlorofluorescein DA; 144489-09-8
CAS-Number: C₂₅H₁₄Cl₂O₉
Molecular Formula: 529.28
Molecular Weight

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Product Data
Handling
Safety Information
Details

Specifications

Purity: ≥95% (NMR)
Appearance: Off-white powder
Identity: 1H-NMR

Properties

Solvents: DMSO, DMF, acetonitrile, methanol, chloroform

Shipping: AMBIENT
Short Term Storage: +4°C
Long Term Storage: -20°C
Handling Advice: Protect from light and moisture.
Use / Stability: Stable for at least 2 years after receipt when stored at -20°C.

Transportation: Not dangerous goods

Description: Non-activated, amine-reactive fluorescent probe. Single 5-isomer of CDCFDA that can be used in biomolecules conjugation. CDCFDA is an amine–reactive fluorescein diacetate (FDA) derivative used to prepare a variety of FDA conjugates. CDCFDA is a non-fluorescent molecule that diffuse into cells and is hydrolyzed by intracellular non-specific esterases, yielding a fluorescent probe. The probe accumulates exclusively in viable cells with intact cell membranes. CFCFDA does not stain dead cells. CDCFDA labeling can be used for monitoring cells by flow cytometry or fluorescence microscopy. The probe is also frequently used to monitor reactive oxygen species (ROS) generation in live cells and intracellular pH levels. Spectral data: ?ex=504nm, ?em=529nm.
Smiles: ClC1=C(OC(C)=O)C=C2C(C3(C(C=CC(C(O)=O)=C4)=C4C(O3)=O)C(C=C(Cl)C(OC(C)=O)=C5)=C5O2)=C1
InChi Key: AJIVVDSJIOOZOJ-UHFFFAOYSA-N
References: (1) C.K. Raymond et al. , J. Cell Biol. 111, 877 (1990) , (2) J.P. Kehrer & T. Paraidathathu , Free Rad. Res. Commun. 16, 217 (1992) , (3) Q.P. Lloyd, et al. , J. Biol. Chem. 270, 22445 (1995) , (4) A.L. Inselman, et al. , Curr. Microbiol. 38, 295 (1999) , (5) M.J. Zamek-Gliszczynski, et al. , J. Pharmacol. Exp. Therap. 304, 801 (2003) , (6) L. Li, et al. , Free Radic. Res. 42, 354 (2008) , (7) M. Medhora, et al. , Am. J. Physiol. Lung Cell Mol. Physiol. 294, L902 (2008) , (8) S. Siissalo, et al. , Eur. J. Pharm. Biopharm. 71, 332 (2009) , (9) A.I. Pogue, et al. , Int. J. Mol. Sci. 13, 9615 (2012) , (10) J. Roh, et al. , Redox Biol. 11, 254 (2016) , (11) J.L. Roh, et al. , Cancer Lett. 381, 96 (2016)

Non-activated, amine-reactive fluorescent probe. Single 5-isomer of CDCFDA that can be used in biomolecules conjugation. CDCFDA is an amine–reactive fluorescein diacetate (FDA) derivative used to prepare a variety of FDA conjugates. CDCFDA is a non-fluorescent molecule that diffuse into cells and is hydrolyzed by intracellular non-specific esterases, yielding a fluorescent probe. The probe accumulates exclusively in viable cells with intact cell membranes. CFCFDA does not stain dead cells. CDCFDA labeling can be used for monitoring cells by flow cytometry or fluorescence microscopy. The probe is also frequently used to monitor reactive oxygen species (ROS) generation in live cells and intracellular pH levels. Spectral data: ?ex=504nm, ?em=529nm.

SKU: C0333 Categories: Fluorescent Detection, Fluorescent Detection

Additional information

Additional information

Synonyms	5-CDCFDA, 5-Carboxy-2',7'-dichlorofluorescein diacetate, 5-Carboxy-2',7'-dichlorofluorescein DA
Purity	≥95% (NMR)
Appearance	Off-white powder
CAS-Number	144489-09-8
Molecular Formula	C25H14Cl2O9
Molecular Weight	529.28
Identity	1H-NMR
Solvents	DMSO, DMF, acetonitrile, methanol, chloroform
Smiles	ClC1=C(OC(C)=O)C=C2C(C3(C(C=CC(C(O)=O)=C4)=C4C(O3)=O)C(C=C(Cl)C(OC(C)=O)=C5)=C5O2)=C1
InChi Key	AJIVVDSJIOOZOJ-UHFFFAOYSA-N
Shipping	AMBIENT
Short Term Storage	+4°C
Long Term Storage	-20°C
Handling Advice	Protect from light and moisture.
Use / Stability	Stable for at least 2 years after receipt when stored at -20°C.
Transportation	Not dangerous goods
References	(1) C.K. Raymond et al., J. Cell Biol. 111, 877 (1990), (2) J.P. Kehrer & T. Paraidathathu, Free Rad. Res. Commun. 16, 217 (1992), (3) Q.P. Lloyd, et al., J. Biol. Chem. 270, 22445 (1995), (4) A.L. Inselman, et al., Curr. Microbiol. 38, 295 (1999), (5) M.J. Zamek-Gliszczynski, et al., J. Pharmacol. Exp. Therap. 304, 801 (2003), (6) L. Li, et al., Free Radic. Res. 42, 354 (2008), (7) M. Medhora, et al., Am. J. Physiol. Lung Cell Mol. Physiol. 294, L902 (2008), (8) S. Siissalo, et al., Eur. J. Pharm. Biopharm. 71, 332 (2009), (9) A.I. Pogue, et al., Int. J. Mol. Sci. 13, 9615 (2012), (10) J. Roh, et al., Redox Biol. 11, 254 (2016), (11) J.L. Roh, et al., Cancer Lett. 381, 96 (2016)
Quantity	100 mg, 250 mg, Bulk