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    5-Carboxy-2'

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      5-Carboxy-2′,7′-dichlorofluorescein DA

      SKU
      C0333

      Categories: Fluorescent Detection, Fluorescent Detection

      Synonyms
      5-CDCFDA, 5-Carboxy-2',7'-dichlorofluorescein diacetate , 5-Carboxy-2',7'-dichlorofluorescein DA
      144489-09-8
      CAS-Number
      C25H14Cl2O9
      Molecular Formula
      529.28
      Molecular Weight

      • Product Data
      • Handling
      • Safety Information
      • Details

      Specifications

      Purity
      ≥95% (NMR)
      Identity
      1H-NMR
      Appearance
      Off-white powder

      Properties

      Solvents
      DMSO, DMF, acetonitrile, methanol, chloroform
      Short Term Storage
      +4°C
      Use / Stability
      Stable for at least 2 years after receipt when stored at -20°C.
      Handling Advice
      Protect from light and moisture.
      Long Term Storage
      -20°C
      Shipping
      AMBIENT
      Transportation
      Not dangerous goods
      Description
      Non-activated, amine-reactive fluorescent probe. Single 5-isomer of CDCFDA that can be used in biomolecules conjugation. CDCFDA is an amine–reactive fluorescein diacetate (FDA) derivative used to prepare a variety of FDA conjugates. CDCFDA is a non-fluorescent molecule that diffuse into cells and is hydrolyzed by intracellular non-specific esterases, yielding a fluorescent probe. The probe accumulates exclusively in viable cells with intact cell membranes. CFCFDA does not stain dead cells. CDCFDA labeling can be used for monitoring cells by flow cytometry or fluorescence microscopy. The probe is also frequently used to monitor reactive oxygen species (ROS) generation in live cells and intracellular pH levels. Spectral data: ?ex=504nm, ?em=529nm.
      References
      (1) C.K. Raymond et al. , J. Cell Biol. 111, 877 (1990) , (2) J.P. Kehrer & T. Paraidathathu , Free Rad. Res. Commun. 16, 217 (1992) , (3) Q.P. Lloyd, et al. , J. Biol. Chem. 270, 22445 (1995) , (4) A.L. Inselman, et al. , Curr. Microbiol. 38, 295 (1999) , (5) M.J. Zamek-Gliszczynski, et al. , J. Pharmacol. Exp. Therap. 304, 801 (2003) , (6) L. Li, et al. , Free Radic. Res. 42, 354 (2008) , (7) M. Medhora, et al. , Am. J. Physiol. Lung Cell Mol. Physiol. 294, L902 (2008) , (8) S. Siissalo, et al. , Eur. J. Pharm. Biopharm. 71, 332 (2009) , (9) A.I. Pogue, et al. , Int. J. Mol. Sci. 13, 9615 (2012) , (10) J. Roh, et al. , Redox Biol. 11, 254 (2016) , (11) J.L. Roh, et al. , Cancer Lett. 381, 96 (2016)
      InChi Key
      AJIVVDSJIOOZOJ-UHFFFAOYSA-N
      Smiles
      ClC1=C(OC(C)=O)C=C2C(C3(C(C=CC(C(O)=O)=C4)=C4C(O3)=O)C(C=C(Cl)C(OC(C)=O)=C5)=C5O2)=C1

      Non-activated, amine-reactive fluorescent probe. Single 5-isomer of CDCFDA that can be used in biomolecules conjugation. CDCFDA is an amine–reactive fluorescein diacetate (FDA) derivative used to prepare a variety of FDA conjugates. CDCFDA is a non-fluorescent molecule that diffuse into cells and is hydrolyzed by intracellular non-specific esterases, yielding a fluorescent probe. The probe accumulates exclusively in viable cells with intact cell membranes. CFCFDA does not stain dead cells. CDCFDA labeling can be used for monitoring cells by flow cytometry or fluorescence microscopy. The probe is also frequently used to monitor reactive oxygen species (ROS) generation in live cells and intracellular pH levels. Spectral data: ?ex=504nm, ?em=529nm.

      SKU: C0333 Categories: Fluorescent Detection, Fluorescent Detection
      • Additional information

      Additional information

      Synonyms

      5-CDCFDA, 5-Carboxy-2',7'-dichlorofluorescein diacetate, 5-Carboxy-2',7'-dichlorofluorescein DA

      Purity

      ≥95% (NMR)

      Appearance

      Off-white powder

      CAS-Number

      144489-09-8

      Molecular Formula

      C25H14Cl2O9

      Molecular Weight

      529.28

      Identity

      1H-NMR

      Solvents

      DMSO, DMF, acetonitrile, methanol, chloroform

      Smiles

      ClC1=C(OC(C)=O)C=C2C(C3(C(C=CC(C(O)=O)=C4)=C4C(O3)=O)C(C=C(Cl)C(OC(C)=O)=C5)=C5O2)=C1

      InChi Key

      AJIVVDSJIOOZOJ-UHFFFAOYSA-N

      Shipping

      AMBIENT

      Short Term Storage

      +4°C

      Long Term Storage

      -20°C

      Handling Advice

      Protect from light and moisture.

      Use / Stability

      Stable for at least 2 years after receipt when stored at -20°C.

      Transportation

      Not dangerous goods

      References

      (1) C.K. Raymond et al., J. Cell Biol. 111, 877 (1990), (2) J.P. Kehrer & T. Paraidathathu, Free Rad. Res. Commun. 16, 217 (1992), (3) Q.P. Lloyd, et al., J. Biol. Chem. 270, 22445 (1995), (4) A.L. Inselman, et al., Curr. Microbiol. 38, 295 (1999), (5) M.J. Zamek-Gliszczynski, et al., J. Pharmacol. Exp. Therap. 304, 801 (2003), (6) L. Li, et al., Free Radic. Res. 42, 354 (2008), (7) M. Medhora, et al., Am. J. Physiol. Lung Cell Mol. Physiol. 294, L902 (2008), (8) S. Siissalo, et al., Eur. J. Pharm. Biopharm. 71, 332 (2009), (9) A.I. Pogue, et al., Int. J. Mol. Sci. 13, 9615 (2012), (10) J. Roh, et al., Redox Biol. 11, 254 (2016), (11) J.L. Roh, et al., Cancer Lett. 381, 96 (2016)

      Quantity

      100 mg, 250 mg, Bulk

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