Available from stock

5-Dodecanoylaminofluorescein

SKU: D0162

Categories: Fluorescent Detection, Fluorescent Detection

Synonyms: AFC12 , DAF , 5-(N-Dodecanoyl)aminofluorescein; 107827-77-0
CAS-Number: C₃₂H₃₅NO₆
Molecular Formula: 529.62
Molecular Weight

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Product Data
Handling
Safety Information
Details

Specifications

Purity: ≥99% (HPLC)
Appearance: Yellow to beige powder
Identity: 1H-NMR

Properties

Solvents: DMF, DMSO

Documentation

Safety Data Sheet (SDS): CDX D0162 MSDS.pdf

Shipping: AMBIENT
Short Term Storage: RT
Long Term Storage: +4°C
Handling Advice: Protect from light and moisture.
Use / Stability: Stable for at least 2 years after receipt when stored at +4°C.

Transportation: Not dangerous goods

Description: Lipophilic fluorescent cell staining probe contains a C12 alkyl chain that will bind to membranes with the fluorophore at the aqueous interface and the alkyl tail protruding into the lipid interior. Useful to label plasma mebranes. Binds strongly to amphiphilic block copolymer micelles. Useful for determining the critical micelle concentration (CMC) of various detergents based on fluorescence polarization (anisotropy). Lipophilic fluorescein probes are commonly utilized for fluorescence recovery after photobleaching (FRAP) measurements of lipid lateral diffusion. Used for visualization of proteins in 1-D and 2-D SDS-PAGE gels. Spectral data: λex=490nm, λem=520nm.
Smiles: O=C1OC2(C3=C(C=C(O)C=C3)OC4=CC(O)=CC=C24)C5=C1C=C(NC(CCCCCCCCCCC)=O)C=C5
InChi Key: VJNRUQFWKRLMSL-UHFFFAOYSA-N
References: (1) D. Beer Stolz, et al., BBRC 184, 160 (1992) , (2) M. Stepanek & K. Prochazka, Langmuir 15, 8800 (1999) , (3) C. Kang, et al., 24, 3297 (2003) , (4) M.D. Wenger, et al., Anal. Biochem. 327, 48 (2005) , (5) M.V. Thorsteinsson, et al., Anal. Biochem. 340, 220 (2005)
InChi: InChI=1S/C32H35NO6/c1-2-3-4-5-6-7-8-9-10-11-30(36)33-21-12-15-25-24(18-21)31(37)39-32(25)26-16-13-22(34)19-28(26)38-29-20-23(35)14-17-27(29)32/h12-20,34-35H,2-11H2,1H3,(H,33,36)

Lipophilic fluorescent cell staining probe contains a C12 alkyl chain that will bind to membranes with the fluorophore at the aqueous interface and the alkyl tail protruding into the lipid interior. Useful to label plasma mebranes. Binds strongly to amphiphilic block copolymer micelles. Useful for determining the critical micelle concentration (CMC) of various detergents based on fluorescence polarization (anisotropy). Lipophilic fluorescein probes are commonly utilized for fluorescence recovery after photobleaching (FRAP) measurements of lipid lateral diffusion. Used for visualization of proteins in 1-D and 2-D SDS-PAGE gels. Spectral data: λex=490nm, λem=520nm.

SKU: D0162 Categories: Fluorescent Detection, Fluorescent Detection

Additional information

Additional information

Synonyms	AFC12, DAF, 5-(N-Dodecanoyl)aminofluorescein
Purity	≥99% (HPLC)
Appearance	Yellow to beige powder
CAS-Number	107827-77-0
Molecular Formula	C32H35NO6
Molecular Weight	529.62
Identity	1H-NMR
Solvents	DMF, DMSO
Smiles	O=C1OC2(C3=C(C=C(O)C=C3)OC4=CC(O)=CC=C24)C5=C1C=C(NC(CCCCCCCCCCC)=O)C=C5
InChi Key	VJNRUQFWKRLMSL-UHFFFAOYSA-N
Shipping	AMBIENT
Short Term Storage	RT
Long Term Storage	+4°C
Handling Advice	Protect from light and moisture.
Use / Stability	Stable for at least 2 years after receipt when stored at +4°C.
Transportation	Not dangerous goods
References	(1) D. Beer Stolz, et al., BBRC 184, 160 (1992), (2) M. Stepanek & K. Prochazka, Langmuir 15, 8800 (1999), (3) C. Kang, et al., 24, 3297 (2003), (4) M.D. Wenger, et al., Anal. Biochem. 327, 48 (2005), (5) M.V. Thorsteinsson, et al., Anal. Biochem. 340, 220 (2005)
InChi	InChI=1S/C32H35NO6/c1-2-3-4-5-6-7-8-9-10-11-30(36)33-21-12-15-25-24(18-21)31(37)39-32(25)26-16-13-22(34)19-28(26)38-29-20-23(35)14-17-27(29)32/h12-20,34-35H,2-11H2,1H3,(H,33,36)
Quantity	50 mg, 250 mg, Bulk