Available from stock

5-DTAF hydrochloride

SKU: D0123

Categories: Fluorescent Detection, Fluorescent Detection

Synonyms: 5-[(4,6-Dichlorotriazin-2-yl)amino]fluorescein hydrochloride , NSC378139; 21811-74-5
CAS-Number: C₂₃H₁₂Cl₂N₄O₅ . HCl
Molecular Formula: 531.73
Molecular Weight

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Product Data
Handling
Safety Information
Details

Specifications

Purity: ≥98% (HPLC)
Appearance: Yellow to orange powder
Identity: 1H-NMR

Properties

Solvents: chloroform

Documentation

Safety Data Sheet (SDS): CDX D0123 SDS.pdf

Shipping: AMBIENT
Short Term Storage: +4°C
Long Term Storage: -20°C
Handling Advice: Protect from light and moisture.
Use / Stability: Stable for at least 2 years after receipt when stored at -20°C.

Transportation: Not dangerous goods

Description: 5-DTAF is highly reactive with proteins. Unlike other reactive fluoresceins, 5-DTAF not only reacts with amino groups, but also reacts with thiol groups and even directly reacts with hydroxy groups such as polysaccharides and other alcohols in aqueous solution at pH above 9. Due to its high reactivity, 5-DTAF is also used to label carbohydrates. Spectral properties: Abs/Em = 492/517 nm
Smiles: Cl.OC(=O)C1=C(C=CC(NC2=NC(Cl)=NC(Cl)=N2)=C1)C1=C2C=CC(=O)C=C2OC2=CC(O)=CC=C12
InChi Key: CEKSHCAIHGNWBK-UHFFFAOYSA-N
References: (1) D. Blakeslee & M.G. Baines: J. Immunol. Methods 13, 305 (1976) , (2) D. Blakeslee: J. Immunol. Methods 17, 361 (1977) , (3) R. Siegler, et al.: J .Pharm. Biomed. Anal. 7, 45 (1989) , (4) S. Prigent-Richard, et al.: J. Biomed. Mater. Res. 40, 275 (1998) , (5) K.N. Krahn, et al.: Anal. Biochem. 350, 177 (2006) , (6) M. Ahmed, et al.: J. Ind. Microbiol. Biotechnol. 38, 249 (2011) , (7) S.E. Szczesny, et al.: PLoS One 9, e99588 (2014)
InChi: InChI=1S/C23H12Cl2N4O5.ClH/c24-21-27-22(25)29-23(28-21)26-10-1-4-13(16(7-10)20(32)33)19-14-5-2-11(30)8-17(14)34-18-9-12(31)3-6-15(18)19

5-DTAF is highly reactive with proteins. Unlike other reactive fluoresceins, 5-DTAF not only reacts with amino groups, but also reacts with thiol groups and even directly reacts with hydroxy groups such as polysaccharides and other alcohols in aqueous solution at pH above 9. Due to its high reactivity, 5-DTAF is also used to label carbohydrates. Spectral properties: Abs/Em = 492/517 nm

SKU: D0123 Categories: Fluorescent Detection, Fluorescent Detection

Additional information

Additional information

Synonyms	5-[(4,6-Dichlorotriazin-2-yl)amino]fluorescein hydrochloride, NSC378139
Purity	≥98% (HPLC)
Appearance	Yellow to orange powder
CAS-Number	21811-74-5
Molecular Formula	C23H12Cl2N4O5 . HCl
Molecular Weight	531.73
Identity	1H-NMR
Solvents	chloroform
Smiles	Cl.OC(=O)C1=C(C=CC(NC2=NC(Cl)=NC(Cl)=N2)=C1)C1=C2C=CC(=O)C=C2OC2=CC(O)=CC=C12
InChi Key	CEKSHCAIHGNWBK-UHFFFAOYSA-N
Shipping	AMBIENT
Short Term Storage	+4°C
Long Term Storage	-20°C
Handling Advice	Protect from light and moisture.
Use / Stability	Stable for at least 2 years after receipt when stored at -20°C.
Transportation	Not dangerous goods
References	(1) D. Blakeslee & M.G. Baines: J. Immunol. Methods 13, 305 (1976), (2) D. Blakeslee: J. Immunol. Methods 17, 361 (1977), (3) R. Siegler, et al.: J .Pharm. Biomed. Anal. 7, 45 (1989), (4) S. Prigent-Richard, et al.: J. Biomed. Mater. Res. 40, 275 (1998), (5) K.N. Krahn, et al.: Anal. Biochem. 350, 177 (2006), (6) M. Ahmed, et al.: J. Ind. Microbiol. Biotechnol. 38, 249 (2011), (7) S.E. Szczesny, et al.: PLoS One 9, e99588 (2014)
InChi	InChI=1S/C23H12Cl2N4O5.ClH/c24-21-27-22(25)29-23(28-21)26-10-1-4-13(16(7-10)20(32)33)19-14-5-2-11(30)8-17(14)34-18-9-12(31)3-6-15(18)19
Quantity	500 mg, 2.5 g, Bulk