- Synonyms: 5-[(4,6-Dichlorotriazin-2-yl)amino]fluorescein hydrochloride, NSC378139
- Purity: ≥98% (HPLC)
- Appearance: Yellow to orange powder.
- CAS-Number: 21811-74-5
- Molecular Formula: C23H12Cl2N4O5 . HCl
- Molecular Weight: 531.73
- Identity: 1H-NMR
- Solvents: chloroform
- Smiles: Cl.OC(=O)C1=C(C=CC(NC2=NC(Cl)=NC(Cl)=N2)=C1)C1=C2C=CC(=O)C=C2OC2=CC(O)=CC=C12
- InChi: InChI=1S/C23H12Cl2N4O5.ClH/c24-21-27-22(25)29-23(28-21)26-10-1-4-13(16(7-10)20(32)33)19-14-5-2-11(30)8-17(14)34-18-9-12(31)3-6-15(18)19
- InChi Key: CEKSHCAIHGNWBK-UHFFFAOYSA-N
Available from stock
5-DTAF hydrochloride
SKU: D0123
Category: Fluorescent Detection
- Shipping: AMBIENT
- Short Term Storage: +4°C
- Long Term Storage: -20°C
- Handling Advice: Protect from light and moisture. Keep cool and dry.
- Use / Stability: Stable for at least 2 years after receipt when stored at -20°C.
- Description: 5-DTAF is highly reactive with proteins. Unlike other reactive fluoresceins, 5-DTAF not only reacts with amino groups, but also reacts with thiol groups and even directly reacts with hydroxy groups such as polysaccharides and other alcohols in aqueous solution at pH above 9. Due to its high reactivity, 5-DTAF is also used to label carbohydrates. Spectral properties: Abs/Em = 492/517 nm
- References: (1) D. Blakeslee & M.G. Baines; J. Immunol. Methods 13, 305 (1976), (2) D. Blakeslee; J. Immunol. Methods 17, 361 (1977), (3) R. Siegler, et al.; J .Pharm. Biomed. Anal. 7, 45 (1989), (4) S. Prigent-Richard, et al.; J. Biomed. Mater. Res. 40, 275 (1998), (5) K.N. Krahn, et al.; Anal. Biochem. 350, 177 (2006), (6) M. Ahmed, et al.; J. Ind. Microbiol. Biotechnol. 38, 249 (2011), (7) S.E. Szczesny, et al.; PLoS One 9, e99588 (2014)
5-DTAF is highly reactive with proteins. Unlike other reactive fluoresceins, 5-DTAF not only reacts with amino groups, but also reacts with thiol groups and even directly reacts with hydroxy groups such as polysaccharides and other alcohols in aqueous solution at pH above 9. Due to its high reactivity, 5-DTAF is also used to label carbohydrates. Spectral properties: Abs/Em = 492/517 nm
Additional information
| Quantity | 2.5 g, 500 mg, Bulk |
|---|---|
| Handling Advice | Protect from light and moisture. Keep cool and dry. |
| Availability | 1 |
| CAS-Number | 21811-74-5 |
| InChi | InChI=1S/C23H12Cl2N4O5.ClH/c24-21-27-22(25)29-23(28-21)26-10-1-4-13(16(7-10)20(32)33)19-14-5-2-11(30)8-17(14)34-18-9-12(31)3-6-15(18)19 |
| Molecular Formula | |
| Appearance | Yellow to orange powder. |
| InChi Key | CEKSHCAIHGNWBK-UHFFFAOYSA-N |
| Long Term Storage | -20°C |
| Identity | 1H-NMR |
| Short Term Storage | +4°C |
| Molecular Weight | |
| Pricetable | |
| References | (1) D. Blakeslee & M.G. Baines; J. Immunol. Methods 13, 305 (1976), (2) D. Blakeslee; J. Immunol. Methods 17, 361 (1977), (3) R. Siegler, et al.; J .Pharm. Biomed. Anal. 7, 45 (1989), (4) S. Prigent-Richard, et al.; J. Biomed. Mater. Res. 40, 275 (1998), (5) K.N. Krahn, et al.; Anal. Biochem. 350, 177 (2006), (6) M. Ahmed, et al.; J. Ind. Microbiol. Biotechnol. 38, 249 (2011), (7) S.E. Szczesny, et al.; PLoS One 9, e99588 (2014) |
| Shipping | AMBIENT |
| Smiles | Cl.OC(=O)C1=C(C=CC(NC2=NC(Cl)=NC(Cl)=N2)=C1)C1=C2C=CC(=O)C=C2OC2=CC(O)=CC=C12 |
| Use / Stability | Stable for at least 2 years after receipt when stored at -20°C. |
| Purity | |
| Solvents | chloroform |
| Synonyms | 5-[(4,6-Dichlorotriazin-2-yl)amino]fluorescein hydrochloride, NSC378139 |
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