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    5-DTAF hydrochloride

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    5-DTAF hydrochloride

    SKU: D0123

    Category: Fluorescent Detection

    • Product Data
    • Handling
    • Details
    • Synonyms: 5-[(4,6-Dichlorotriazin-2-yl)amino]fluorescein hydrochloride, NSC378139
    • Purity: ≥98% (HPLC)
    • Appearance: Yellow to orange powder.
    • CAS-Number: 21811-74-5
    • Molecular Formula: C23H12Cl2N4O5 . HCl
    • Molecular Weight: 531.73
    • Identity: 1H-NMR
    • Solvents: chloroform
    • Smiles: Cl.OC(=O)C1=C(C=CC(NC2=NC(Cl)=NC(Cl)=N2)=C1)C1=C2C=CC(=O)C=C2OC2=CC(O)=CC=C12
    • InChi: InChI=1S/C23H12Cl2N4O5.ClH/c24-21-27-22(25)29-23(28-21)26-10-1-4-13(16(7-10)20(32)33)19-14-5-2-11(30)8-17(14)34-18-9-12(31)3-6-15(18)19
    • InChi Key: CEKSHCAIHGNWBK-UHFFFAOYSA-N
    • Shipping: AMBIENT
    • Short Term Storage: +4°C
    • Long Term Storage: -20°C
    • Handling Advice: Protect from light and moisture. Keep cool and dry.
    • Use / Stability: Stable for at least 2 years after receipt when stored at -20°C.
    • Description: 5-DTAF is highly reactive with proteins. Unlike other reactive fluoresceins, 5-DTAF not only reacts with amino groups, but also reacts with thiol groups and even directly reacts with hydroxy groups such as polysaccharides and other alcohols in aqueous solution at pH above 9. Due to its high reactivity, 5-DTAF is also used to label carbohydrates. Spectral properties: Abs/Em = 492/517 nm
    • References: (1) D. Blakeslee & M.G. Baines; J. Immunol. Methods 13, 305 (1976), (2) D. Blakeslee; J. Immunol. Methods 17, 361 (1977), (3) R. Siegler, et al.; J .Pharm. Biomed. Anal. 7, 45 (1989), (4) S. Prigent-Richard, et al.; J. Biomed. Mater. Res. 40, 275 (1998), (5) K.N. Krahn, et al.; Anal. Biochem. 350, 177 (2006), (6) M. Ahmed, et al.; J. Ind. Microbiol. Biotechnol. 38, 249 (2011), (7) S.E. Szczesny, et al.; PLoS One 9, e99588 (2014)

    5-DTAF is highly reactive with proteins. Unlike other reactive fluoresceins, 5-DTAF not only reacts with amino groups, but also reacts with thiol groups and even directly reacts with hydroxy groups such as polysaccharides and other alcohols in aqueous solution at pH above 9. Due to its high reactivity, 5-DTAF is also used to label carbohydrates. Spectral properties: Abs/Em = 492/517 nm

    SKU: D0123 Category: Fluorescent Detection
    • Additional information
    • Reviews (0)

    Additional information

    Quantity

    2.5 g, 500 mg, Bulk

    Handling Advice

    Protect from light and moisture. Keep cool and dry.

    Availability

    1

    CAS-Number

    21811-74-5

    InChi

    InChI=1S/C23H12Cl2N4O5.ClH/c24-21-27-22(25)29-23(28-21)26-10-1-4-13(16(7-10)20(32)33)19-14-5-2-11(30)8-17(14)34-18-9-12(31)3-6-15(18)19

    Molecular Formula

    C23H12Cl2N4O5 . HCl

    Appearance

    Yellow to orange powder.

    InChi Key

    CEKSHCAIHGNWBK-UHFFFAOYSA-N

    Long Term Storage

    -20°C

    Identity

    1H-NMR

    Short Term Storage

    +4°C

    Molecular Weight

    531.73

    Pricetable

    2.5 g_EUR_480, 500 mg_EUR_120

    References

    (1) D. Blakeslee & M.G. Baines; J. Immunol. Methods 13, 305 (1976), (2) D. Blakeslee; J. Immunol. Methods 17, 361 (1977), (3) R. Siegler, et al.; J .Pharm. Biomed. Anal. 7, 45 (1989), (4) S. Prigent-Richard, et al.; J. Biomed. Mater. Res. 40, 275 (1998), (5) K.N. Krahn, et al.; Anal. Biochem. 350, 177 (2006), (6) M. Ahmed, et al.; J. Ind. Microbiol. Biotechnol. 38, 249 (2011), (7) S.E. Szczesny, et al.; PLoS One 9, e99588 (2014)

    Shipping

    AMBIENT

    Smiles

    Cl.OC(=O)C1=C(C=CC(NC2=NC(Cl)=NC(Cl)=N2)=C1)C1=C2C=CC(=O)C=C2OC2=CC(O)=CC=C12

    Use / Stability

    Stable for at least 2 years after receipt when stored at -20°C.

    Purity

    ≥98% (HPLC)

    Solvents

    chloroform

    Synonyms

    5-[(4,6-Dichlorotriazin-2-yl)amino]fluorescein hydrochloride, NSC378139

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    Dihydrofluorescein diacetate Dihydrofluorescein diacetate 3,3'-Dipropylthiadicarbocyanine iodide 3,3′-Dipropylthiadicarbocyanine iodide
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