Specifications
- Purity
- ≥98% (HPLC)
- Identity
- 1H-NMR
- Appearance
- Yellow to orange powder
Properties
- Solvents
- chloroform
Available from stock
5-DTAF hydrochloride
- SKU
- D0123
Category: Fluorescent Detection
- Synonyms
- 5-[(4,6-Dichlorotriazin-2-yl)amino]fluorescein hydrochloride , NSC378139
- 21811-74-5
- CAS-Number
- C23H12Cl2N4O5 . HCl
- Molecular Formula
- 531.73
- Molecular Weight
- Short Term Storage
- +4°C
- Use / Stability
- Stable for at least 2 years after receipt when stored at -20°C.
- Handling Advice
- Protect from light and moisture.
- Long Term Storage
- -20°C
- Shipping
- AMBIENT
- Transportation
- Not dangerous goods
- Description
- 5-DTAF is highly reactive with proteins. Unlike other reactive fluoresceins, 5-DTAF not only reacts with amino groups, but also reacts with thiol groups and even directly reacts with hydroxy groups such as polysaccharides and other alcohols in aqueous solution at pH above 9. Due to its high reactivity, 5-DTAF is also used to label carbohydrates. Spectral properties: Abs/Em = 492/517 nm
- InChi
- InChI=1S/C23H12Cl2N4O5.ClH/c24-21-27-22(25)29-23(28-21)26-10-1-4-13(16(7-10)20(32)33)19-14-5-2-11(30)8-17(14)34-18-9-12(31)3-6-15(18)19
- References
- (1) D. Blakeslee & M.G. Baines: J. Immunol. Methods 13, 305 (1976) , (2) D. Blakeslee: J. Immunol. Methods 17, 361 (1977) , (3) R. Siegler, et al.: J .Pharm. Biomed. Anal. 7, 45 (1989) , (4) S. Prigent-Richard, et al.: J. Biomed. Mater. Res. 40, 275 (1998) , (5) K.N. Krahn, et al.: Anal. Biochem. 350, 177 (2006) , (6) M. Ahmed, et al.: J. Ind. Microbiol. Biotechnol. 38, 249 (2011) , (7) S.E. Szczesny, et al.: PLoS One 9, e99588 (2014)
- InChi Key
- CEKSHCAIHGNWBK-UHFFFAOYSA-N
- Smiles
- Cl.OC(=O)C1=C(C=CC(NC2=NC(Cl)=NC(Cl)=N2)=C1)C1=C2C=CC(=O)C=C2OC2=CC(O)=CC=C12
5-DTAF is highly reactive with proteins. Unlike other reactive fluoresceins, 5-DTAF not only reacts with amino groups, but also reacts with thiol groups and even directly reacts with hydroxy groups such as polysaccharides and other alcohols in aqueous solution at pH above 9. Due to its high reactivity, 5-DTAF is also used to label carbohydrates. Spectral properties: Abs/Em = 492/517 nm
Additional information
| Synonyms | 5-[(4,6-Dichlorotriazin-2-yl)amino]fluorescein hydrochloride, NSC378139 |
|---|---|
| Purity | ≥98% (HPLC) |
| Appearance | Yellow to orange powder |
| CAS-Number | 21811-74-5 |
| Molecular Formula | C23H12Cl2N4O5 . HCl |
| Molecular Weight | 531.73 |
| Identity | 1H-NMR |
| Solvents | chloroform |
| Smiles | Cl.OC(=O)C1=C(C=CC(NC2=NC(Cl)=NC(Cl)=N2)=C1)C1=C2C=CC(=O)C=C2OC2=CC(O)=CC=C12 |
| InChi Key | CEKSHCAIHGNWBK-UHFFFAOYSA-N |
| Shipping | AMBIENT |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice | Protect from light and moisture. |
| Use / Stability | Stable for at least 2 years after receipt when stored at -20°C. |
| Transportation | Not dangerous goods |
| References | (1) D. Blakeslee & M.G. Baines: J. Immunol. Methods 13, 305 (1976), (2) D. Blakeslee: J. Immunol. Methods 17, 361 (1977), (3) R. Siegler, et al.: J .Pharm. Biomed. Anal. 7, 45 (1989), (4) S. Prigent-Richard, et al.: J. Biomed. Mater. Res. 40, 275 (1998), (5) K.N. Krahn, et al.: Anal. Biochem. 350, 177 (2006), (6) M. Ahmed, et al.: J. Ind. Microbiol. Biotechnol. 38, 249 (2011), (7) S.E. Szczesny, et al.: PLoS One 9, e99588 (2014) |
| InChi | InChI=1S/C23H12Cl2N4O5.ClH/c24-21-27-22(25)29-23(28-21)26-10-1-4-13(16(7-10)20(32)33)19-14-5-2-11(30)8-17(14)34-18-9-12(31)3-6-15(18)19 |
| Quantity | 500 mg, 2.5 g, Bulk |
