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    5-FITC

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      5-FITC

      SKU
      F0011

      Categories: Fluorescent Detection, Fluorescent Detection

      Synonyms
      Fluorescein 5-isothiocyanate
      3326-32-7
      CAS-Number
      C21H11NO5S
      Molecular Formula
      389.38
      Molecular Weight

      For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

      • Product Data
      • Handling
      • Safety Information
      • Details

      Specifications

      Purity
      ≥95% (HPLC)
      Appearance
      Yellow to orange powder
      Identity
      1H-NMR

      Properties

      Solvents
      aqueous buffers (pH ≥ 6)
      Melting Point
      >360 °C
      Fluorescence
      λex 492 nm, λem 518 nm in 0,1 M phosphate pH 8.0

      Documentation

      Safety Data Sheet (SDS)
      CDX F0011 MSDS.pdf
      Shipping
      AMBIENT
      Short Term Storage
      +4°C
      Long Term Storage
      -20°C
      Handling Advice
      Protect from light and moisture.
      Use / Stability
      Stable for at least 2 years after receipt when stored at -20°C.
      Hazard statements
      H317, H334
      Precautionary statements
      P261, P280, P342 + P311
      GHS Symbol
      GHS08
      Signal word
      Danger
      Transportation
      Not dangerous goods
      Description
      Fluorescent marker for biochemical applications. Reacts under mild conditions with primary amines. Used in modification of actin at lys-61, labelling of FAB and the modification of thiol groups.
      Smiles
      OC1=CC2=C(C=C1)C1(OC(=O)C3=C1C=CC(=C3)N=C=S)C1=C(O2)C=C(O)C=C1
      InChi Key
      MHMNJMPURVTYEJ-UHFFFAOYSA-N
      References
      (1) D-W. Han et al.: Bioorg.& Med. Chem. 16(22), 9652 (2008) , (2) L. Miller et al.: Eur. J. Biochem. 174, 23 (1998) , (3) G.P. Der-Balian et al.: Anal. Biochem. 173, 59 (1988) , (4) K. Muramoto et al.: Anal. Biochem. 141, 446 (1984) , (5) A.F. Wilderspin et al.: Anal. Biochem. 132, 449 (1983)
      InChi
      InChI=1S/C21H11NO5S/c23-12-2-5-16-18(8-12)26-19-9-13(24)3-6-17(19)21(16)15-4-1-11(22-10-28)7-14(15)20(25)27-21/h1-9,23-24H

      Fluorescent marker for biochemical applications. Reacts under mild conditions with primary amines. Used in modification of actin at lys-61, labelling of FAB and the modification of thiol groups.

      SKU: F0011 Categories: Fluorescent Detection, Fluorescent Detection
      • Additional information

      Additional information

      Synonyms

      Fluorescein 5-isothiocyanate

      Purity

      ≥95% (HPLC)

      Appearance

      Yellow to orange powder

      CAS-Number

      3326-32-7

      Molecular Formula

      C21H11NO5S

      Molecular Weight

      389.38

      Identity

      1H-NMR

      Solvents

      aqueous buffers (pH ≥ 6)

      Melting Point

      >360 °C

      Fluorescence

      λex 492 nm, λem 518 nm in 0,1 M phosphate pH 8.0

      Smiles

      OC1=CC2=C(C=C1)C1(OC(=O)C3=C1C=CC(=C3)N=C=S)C1=C(O2)C=C(O)C=C1

      InChi Key

      MHMNJMPURVTYEJ-UHFFFAOYSA-N

      Shipping

      AMBIENT

      Short Term Storage

      +4°C

      Long Term Storage

      -20°C

      Handling Advice

      Protect from light and moisture.

      Use / Stability

      Stable for at least 2 years after receipt when stored at -20°C.

      Hazard statements

      H317, H334

      Precautionary statements

      P261, P280, P342 + P311

      GHS Symbol

      GHS08

      Signal word

      Danger

      Transportation

      Not dangerous goods

      References

      (1) D-W. Han et al.: Bioorg.& Med. Chem. 16(22), 9652 (2008), (2) L. Miller et al.: Eur. J. Biochem. 174, 23 (1998), (3) G.P. Der-Balian et al.: Anal. Biochem. 173, 59 (1988), (4) K. Muramoto et al.: Anal. Biochem. 141, 446 (1984), (5) A.F. Wilderspin et al.: Anal. Biochem. 132, 449 (1983)

      InChi

      InChI=1S/C21H11NO5S/c23-12-2-5-16-18(8-12)26-19-9-13(24)3-6-17(19)21(16)15-4-1-11(22-10-28)7-14(15)20(25)27-21/h1-9,23-24H

      Quantity

      250 mg, 1 g, 10 g, Bulk

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