5-Hexadecanoylaminofluorescein

SKU: H0028

Categories: Fluorescent Detection, Fluorescent Detection

Synonyms: AFC16 , HAF , HEDAF; 73024-80-3
CAS-Number: C₃₆H₄₃NO₆
Molecular Formula: 585.73
Molecular Weight

Product Data
Handling
Safety Information
Details

Specifications

Purity: ≥97% (HPLC)
Identity: 1H-NMR
Appearance: Beige powder

Properties

Solvents: water (slightly)

Downloads

Safety Data Sheet: CDX H0028 MSDS.pdf

Short Term Storage: RT
Use / Stability: Stable for at least 2 years after receipt when stored at +4°C.
Handling Advice: Protect from light and moisture.
Long Term Storage: +4°C
Shipping: AMBIENT

Description: Lipophilic and pH-sensitive green-emitting fluorescent cell staining probe contains a C16 alkyl chain that will bind to membranes with the fluorophore at the aqueous interface and the alkyl tail protruding into the lipid interior. Useful to label plasma mebranes. Can be used to monitor surface pH by two-wavelength micro-spectrofluorometry. Used for visualization of proteins in 1-D and 2-D SDS-PAGE gels. Spectral data: λex=497nm, λem=520nm.
InChi: InChI=1S/C36H43NO6/c1-2-3-4-5-6-7-8-9-10-11-12-13-14-15-34(40)37-25-16-19-29-28(22-25)35(41)43-36(29)30-20-17-26(38)23-32(30)42-33-24-27(39)18-21-31(33)36/h16-24,38-39H,2-15H2,1H3,(H,37,40)
References: (1) L.A. Sklar, et al., Biochemistry 19, 1294 (1980) , (2) D. Beer Stolz, et al., BBRC 184, 160 (1992) , (3) A.-K. Genz, et al., Comp. Biochem. Physiol. Vol. 118A, 407 (1997) , (4) F. Chevalier, et al., Phytochem. 65, 1499 (2004) , (5) X. Wang, et al., Angew. Chem. Int. Ed. 52, 406 (2013) , (6) J. Jacoby, et al., J. Neurophysiol. 111, 1056 (2014)
InChi Key: QWSRVJGBAIRGMP-UHFFFAOYSA-N
Smiles: O=C1OC2(C3=C(C=C(O)C=C3)OC4=CC(O)=CC=C24)C5=C1C=C(NC(CCCCCCCCCCCCCCC)=O)C=C5

Lipophilic and pH-sensitive green-emitting fluorescent cell staining probe contains a C16 alkyl chain that will bind to membranes with the fluorophore at the aqueous interface and the alkyl tail protruding into the lipid interior. Useful to label plasma mebranes. Can be used to monitor surface pH by two-wavelength micro-spectrofluorometry. Used for visualization of proteins in 1-D and 2-D SDS-PAGE gels. Spectral data: λex=497nm, λem=520nm.

SKU: H0028 Categories: Fluorescent Detection, Fluorescent Detection

Additional information

Additional information

Synonyms	AFC16, HAF, HEDAF
Purity	≥97% (HPLC)
Appearance	Beige powder
CAS-Number	73024-80-3
Molecular Formula	C36H43NO6
Molecular Weight	585.73
Identity	1H-NMR
Solvents	water (slightly)
Smiles	O=C1OC2(C3=C(C=C(O)C=C3)OC4=CC(O)=CC=C24)C5=C1C=C(NC(CCCCCCCCCCCCCCC)=O)C=C5
InChi Key	QWSRVJGBAIRGMP-UHFFFAOYSA-N
Shipping	AMBIENT
Short Term Storage	RT
Long Term Storage	+4°C
Handling Advice	Protect from light and moisture.
Use / Stability	Stable for at least 2 years after receipt when stored at +4°C.
References	(1) L.A. Sklar, et al., Biochemistry 19, 1294 (1980), (2) D. Beer Stolz, et al., BBRC 184, 160 (1992), (3) A.-K. Genz, et al., Comp. Biochem. Physiol. Vol. 118A, 407 (1997), (4) F. Chevalier, et al., Phytochem. 65, 1499 (2004), (5) X. Wang, et al., Angew. Chem. Int. Ed. 52, 406 (2013), (6) J. Jacoby, et al., J. Neurophysiol. 111, 1056 (2014)
InChi	InChI=1S/C36H43NO6/c1-2-3-4-5-6-7-8-9-10-11-12-13-14-15-34(40)37-25-16-19-29-28(22-25)35(41)43-36(29)30-20-17-26(38)23-32(30)42-33-24-27(39)18-21-31(33)36/h16-24,38-39H,2-15H2,1H3,(H,37,40)
Quantity	50 mg, 250 mg, Bulk