Specifications
- Purity
- ≥98% (NMR)
- Appearance
- Orange powder
- Identity
- 1H-NMR
Properties
- Solvents
- Soluble in DMSO or ethanol.
- Fluorescence
- λex 497 nm, λem 520 nm±10 nm in ethanol
Available from stock
5-(Octadecanoylamino)fluorescein
- SKU
- O0013
Categories: Fluorescent Detection, Fluorescent Detection
- Synonyms
- 5-(Stearoylamino)fluorescein, ODAF
- 110698-53-8
- CAS-Number
- C38H47NO6
- Molecular Formula
- 613.78
- Molecular Weight
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
- Shipping
- AMBIENT
- Short Term Storage
- +4°C
- Long Term Storage
- +4°C
- Handling Advice
- Protect from light and moisture.
- Use / Stability
- Stable for at least 2 years after receipt when stored at +4°C.
- Transportation
- Not dangerous goods
- Description
- 4-Methylumbelliferyl palmitate (4-MUP) is a fluorogenic substrate for lysosomal acid lypase (LAL). 4-MUP is cleaved by LAL (or by other acid lipases) to release the fluorescent moiety 4-MU. Recent advances allow the assessment of LAL activity in very small blood volumes using 4-MUP. Cholesterol ester storage disease and Wolman disease are recessive autosomal disorders caused by a deficiency in lysosomal acid lipase (LAL), also known as cholesteryl ester hydrolase. Spectral data: 4-MU fluorescence is pH-dependent. λex 320nm (pH 1.97-6.72) and 360nm (pH 7.12-10.3); λem 445-455nM (increasing as pH decreases).
- Smiles
- O=C1C=C2OC3=CC(O)=CC=C3C(C4=C(C(O)=O)C=C(NC(CCCCCCCCCCCCCCCCC)=O)C=C4)=C2C=C1
- InChi Key
- XKFGZSAKZAPLGI-UHFFFAOYSA-N
- References
- (1) G.J. Guy & J. Butterworth; Clin. Chim. Acta 84, 361 (1978) , (2) S. Kelly & R. Bakhru-Kishore; Clin. Chim. Acta 97, 239 (1979) , (3) T.G. Warner, et al.; Am. J. Hum. Gen. 32, 869 (1980) , (4) T.G. Warner, et al.; J. Biol. Chem. 256, 2952 (1981) , (5) M.W. Baillargeon & S.G. McCarthy; Lipids 26, 831 (1991) , (6) J. Hamilton, et al.; Clin. Chim. Acta 413, 1207 (2012) , (7) H. Zhi, et al.; J. Spectrosc. 1, 147128 (2013) , (8) T. Dairaku, et al.; Mol. Gen. Metab. 111, 193 (2014)
4-Methylumbelliferyl palmitate (4-MUP) is a fluorogenic substrate for lysosomal acid lypase (LAL). 4-MUP is cleaved by LAL (or by other acid lipases) to release the fluorescent moiety 4-MU. Recent advances allow the assessment of LAL activity in very small blood volumes using 4-MUP. Cholesterol ester storage disease and Wolman disease are recessive autosomal disorders caused by a deficiency in lysosomal acid lipase (LAL), also known as cholesteryl ester hydrolase. Spectral data: 4-MU fluorescence is pH-dependent. λex 320nm (pH 1.97-6.72) and 360nm (pH 7.12-10.3); λem 445-455nM (increasing as pH decreases).
Additional information
| Synonyms | 5-(Stearoylamino)fluorescein, ODAF |
|---|---|
| Purity | ≥98% (NMR) |
| Appearance | Orange powder |
| CAS-Number | 110698-53-8 |
| Molecular Formula | C38H47NO6 |
| Molecular Weight | 613.78 |
| Identity | 1H-NMR |
| Solvents | Soluble in DMSO or ethanol. |
| Fluorescence | λex 497 nm, λem 520 nm±10 nm in ethanol |
| Smiles | O=C1C=C2OC3=CC(O)=CC=C3C(C4=C(C(O)=O)C=C(NC(CCCCCCCCCCCCCCCCC)=O)C=C4)=C2C=C1 |
| InChi Key | XKFGZSAKZAPLGI-UHFFFAOYSA-N |
| Shipping | AMBIENT |
| Short Term Storage | +4°C |
| Long Term Storage | +4°C |
| Handling Advice | Protect from light and moisture. |
| Use / Stability | Stable for at least 2 years after receipt when stored at +4°C. |
| Transportation | Not dangerous goods |
| References | (1) G.J. Guy & J. Butterworth; Clin. Chim. Acta 84, 361 (1978), (2) S. Kelly & R. Bakhru-Kishore; Clin. Chim. Acta 97, 239 (1979), (3) T.G. Warner, et al.; Am. J. Hum. Gen. 32, 869 (1980), (4) T.G. Warner, et al.; J. Biol. Chem. 256, 2952 (1981), (5) M.W. Baillargeon & S.G. McCarthy; Lipids 26, 831 (1991), (6) J. Hamilton, et al.; Clin. Chim. Acta 413, 1207 (2012), (7) H. Zhi, et al.; J. Spectrosc. 1, 147128 (2013), (8) T. Dairaku, et al.; Mol. Gen. Metab. 111, 193 (2014) |
| Quantity | 100 mg, Bulk |
