Specifications
- Purity
- ≥95% (HPLC)
- Appearance
- Orange powder
- Identity
- 1H-NMR
Properties
- Solvents
- DMSO, DMF, methanol
Documentation
- Safety Data Sheet (SDS)
- CDX F0035 MSDS_V1.1.pdf
Available from stock
5-SFX SE
- SKU
- F0035
Categories: Fluorescent Detection, Fluorescent Detection
- Synonyms
- 5-FAM-X SE , Fluorescein-5-carboxamidocaproic acid N-succinimidyl ester , 6-(Fluorescein-5-carboxamido)hexanoic acid N-succinimidyl ester
- 148356-00-7
- CAS-Number
- C31H26N2O10
- Molecular Formula
- 586.55
- Molecular Weight
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
- Shipping
- AMBIENT
- Short Term Storage
- -20°C
- Long Term Storage
- -20°C
- Handling Advice
- Protect from light and moisture.
- Use / Stability
- Stable for at least 2 years after receipt when stored at -20°C.
- Hazard statements
- H315, H319, H335
- Precautionary statements
- P261, P305 + P351 + P338
- GHS Symbol
- GHS07
- Signal word
- Warning
- Transportation
- Not dangerous goods
- Description
- Amine-reactive fluorescent probe for introducing a fluorescein label at a nucleophilic residue of a target molecule. This fluorescein labeling reagent has an added (X) spacer to improve accessibility of the reactive group by nucleophiles in preparation of bioconjugates. The reactive N-hydroxysuccinimide (NHS) group can be used for easy attachment to proteins or amine containing nucleotides using simple procedures. Spectral properties, lambdaex 490 nm, lambdaem 514 nm in DMF (pH 8.2) and lambdaex 491 nm, lambdaem 515 nm in 0.1 M Tris pH 9.0.
- Smiles
- OC1=CC2=C(C(C3=CC=C(C(NCCCCCC(ON4C(CCC4=O)=O)=O)=O)C=C3C(O)=O)=C(C=C5)C(O2)=CC5=O)C=C1
- InChi Key
- GQROVDGSBTYULQ-UHFFFAOYSA-N
- References
- (1) G.P. Jamieson, et al., Cytometry 14, 32 (1993) , (2) K.A. Cruickshank, Anal. Biochem. 269, 21 (1999) , (3) J.J. MacLean, et al., Biophys. J. 78, 1441 (2000) , (4) W.C. Cooper, et al., Biophys. J. 78, 1449 (2000) , (5) M.P. Gygi, et al., Nucleic Acids Res. 30, 2790 (2002)
- InChi
- InChI=1S/C31H26N2O10/c34-18-6-9-21-24(15-18)42-25-16-19(35)7-10-22(25)29(21)20-8-5-17(14-23(20)31(40)41)30(39)32-13-3-1-2-4-28(38)43-33-26(36)11-12-27(33)37/h5-10,14-16,34H,1-4,11-13H2,(H,32,39)(H,40,41)
Amine-reactive fluorescent probe for introducing a fluorescein label at a nucleophilic residue of a target molecule. This fluorescein labeling reagent has an added (X) spacer to improve accessibility of the reactive group by nucleophiles in preparation of bioconjugates. The reactive N-hydroxysuccinimide (NHS) group can be used for easy attachment to proteins or amine containing nucleotides using simple procedures. Spectral properties, lambdaex 490 nm, lambdaem 514 nm in DMF (pH 8.2) and lambdaex 491 nm, lambdaem 515 nm in 0.1 M Tris pH 9.0.
Additional information
| Synonyms | 5-FAM-X SE, Fluorescein-5-carboxamidocaproic acid N-succinimidyl ester, 6-(Fluorescein-5-carboxamido)hexanoic acid N-succinimidyl ester |
|---|---|
| Purity | ≥95% (HPLC) |
| Appearance | Orange powder |
| CAS-Number | 148356-00-7 |
| Molecular Formula | C31H26N2O10 |
| Molecular Weight | 586.55 |
| Identity | 1H-NMR |
| Solvents | DMSO, DMF, methanol |
| Smiles | OC1=CC2=C(C(C3=CC=C(C(NCCCCCC(ON4C(CCC4=O)=O)=O)=O)C=C3C(O)=O)=C(C=C5)C(O2)=CC5=O)C=C1 |
| InChi Key | GQROVDGSBTYULQ-UHFFFAOYSA-N |
| Shipping | AMBIENT |
| Short Term Storage | -20°C |
| Long Term Storage | -20°C |
| Handling Advice | Protect from light and moisture. |
| Use / Stability | Stable for at least 2 years after receipt when stored at -20°C. |
| Hazard statements | H315, H319, H335 |
| Precautionary statements | P261, P305 + P351 + P338 |
| GHS Symbol | GHS07 |
| Signal word | Warning |
| Transportation | Not dangerous goods |
| References | (1) G.P. Jamieson, et al., Cytometry 14, 32 (1993), (2) K.A. Cruickshank, Anal. Biochem. 269, 21 (1999), (3) J.J. MacLean, et al., Biophys. J. 78, 1441 (2000), (4) W.C. Cooper, et al., Biophys. J. 78, 1449 (2000), (5) M.P. Gygi, et al., Nucleic Acids Res. 30, 2790 (2002) |
| InChi | InChI=1S/C31H26N2O10/c34-18-6-9-21-24(15-18)42-25-16-19(35)7-10-22(25)29(21)20-8-5-17(14-23(20)31(40)41)30(39)32-13-3-1-2-4-28(38)43-33-26(36)11-12-27(33)37/h5-10,14-16,34H,1-4,11-13H2,(H,32,39)(H,40,41) |
| Quantity | 10 mg, 25 mg, Bulk |
