Specifications
- Purity
- ≥95% (HPLC)
- Identity
- 1H-NMR
- Appearance
- Orange powder
Properties
- Solvents
- DMSO, DMF, methanol
Available from stock
5-SFX SE
- SKU
- F0035
Category: Fluorescent Detection
- Synonyms
- 5-FAM-X SE , Fluorescein-5-carboxamidocaproic acid N-succinimidyl ester , 6-(Fluorescein-5-carboxamido)hexanoic acid N-succinimidyl ester
- 148356-00-7
- CAS-Number
- C31H26N2O10
- Molecular Formula
- 586.55
- Molecular Weight
- Handling Advice
- Protect from light and moisture.
- Use / Stability
- Stable for at least 2 years after receipt when stored at -20°C.
- Long Term Storage
- -20°C
- Short Term Storage
- -20°C
- Shipping
- AMBIENT
- Transportation
- Not dangerous goods
- Signal word
- Warning
- GHS Symbol
- GHS07
- Precautionary statements
- P261, P305 + P351 + P338
- Hazard statements
- H315, H319, H335
- Description
- Amine-reactive fluorescent probe for introducing a fluorescein label at a nucleophilic residue of a target molecule. This fluorescein labeling reagent has an added (X) spacer to improve accessibility of the reactive group by nucleophiles in preparation of bioconjugates. The reactive N-hydroxysuccinimide (NHS) group can be used for easy attachment to proteins or amine containing nucleotides using simple procedures. Spectral properties, lambdaex 490 nm, lambdaem 514 nm in DMF (pH 8.2) and lambdaex 491 nm, lambdaem 515 nm in 0.1 M Tris pH 9.0.
- InChi
- InChI=1S/C31H26N2O10/c34-18-6-9-21-24(15-18)42-25-16-19(35)7-10-22(25)29(21)20-8-5-17(14-23(20)31(40)41)30(39)32-13-3-1-2-4-28(38)43-33-26(36)11-12-27(33)37/h5-10,14-16,34H,1-4,11-13H2,(H,32,39)(H,40,41)
- References
- (1) G.P. Jamieson, et al., Cytometry 14, 32 (1993) , (2) K.A. Cruickshank, Anal. Biochem. 269, 21 (1999) , (3) J.J. MacLean, et al., Biophys. J. 78, 1441 (2000) , (4) W.C. Cooper, et al., Biophys. J. 78, 1449 (2000) , (5) M.P. Gygi, et al., Nucleic Acids Res. 30, 2790 (2002)
- InChi Key
- GQROVDGSBTYULQ-UHFFFAOYSA-N
- Smiles
- OC1=CC2=C(C(C3=CC=C(C(NCCCCCC(ON4C(CCC4=O)=O)=O)=O)C=C3C(O)=O)=C(C=C5)C(O2)=CC5=O)C=C1
Amine-reactive fluorescent probe for introducing a fluorescein label at a nucleophilic residue of a target molecule. This fluorescein labeling reagent has an added (X) spacer to improve accessibility of the reactive group by nucleophiles in preparation of bioconjugates. The reactive N-hydroxysuccinimide (NHS) group can be used for easy attachment to proteins or amine containing nucleotides using simple procedures. Spectral properties, lambdaex 490 nm, lambdaem 514 nm in DMF (pH 8.2) and lambdaex 491 nm, lambdaem 515 nm in 0.1 M Tris pH 9.0.
Additional information
| Synonyms | 5-FAM-X SE, Fluorescein-5-carboxamidocaproic acid N-succinimidyl ester, 6-(Fluorescein-5-carboxamido)hexanoic acid N-succinimidyl ester |
|---|---|
| Purity | ≥95% (HPLC) |
| Appearance | Orange powder |
| CAS-Number | 148356-00-7 |
| Molecular Formula | C31H26N2O10 |
| Molecular Weight | 586.55 |
| Identity | 1H-NMR |
| Solvents | DMSO, DMF, methanol |
| Smiles | OC1=CC2=C(C(C3=CC=C(C(NCCCCCC(ON4C(CCC4=O)=O)=O)=O)C=C3C(O)=O)=C(C=C5)C(O2)=CC5=O)C=C1 |
| InChi Key | GQROVDGSBTYULQ-UHFFFAOYSA-N |
| Shipping | AMBIENT |
| Short Term Storage | -20°C |
| Long Term Storage | -20°C |
| Handling Advice | Protect from light and moisture. |
| Use / Stability | Stable for at least 2 years after receipt when stored at -20°C. |
| Hazard statements | H315, H319, H335 |
| Precautionary statements | P261, P305 + P351 + P338 |
| GHS Symbol | GHS07 |
| Signal word | Warning |
| Transportation | Not dangerous goods |
| References | (1) G.P. Jamieson, et al., Cytometry 14, 32 (1993), (2) K.A. Cruickshank, Anal. Biochem. 269, 21 (1999), (3) J.J. MacLean, et al., Biophys. J. 78, 1441 (2000), (4) W.C. Cooper, et al., Biophys. J. 78, 1449 (2000), (5) M.P. Gygi, et al., Nucleic Acids Res. 30, 2790 (2002) |
| InChi | InChI=1S/C31H26N2O10/c34-18-6-9-21-24(15-18)42-25-16-19(35)7-10-22(25)29(21)20-8-5-17(14-23(20)31(40)41)30(39)32-13-3-1-2-4-28(38)43-33-26(36)11-12-27(33)37/h5-10,14-16,34H,1-4,11-13H2,(H,32,39)(H,40,41) |
| Quantity | 10 mg, 25 mg, Bulk |
