Available from stock

5(6)-Carboxy-2′,7′-difluorofluorescein

SKU: C0262

Category: Fluorescent Detection

251450-64-3
CAS-Number: C₂₁H₁₂F₂O₇
Molecular Formula: 414.32
Molecular Weight

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Product Data
Handling
Safety Information
Details

Specifications

Purity: ≥95% (NMR)
Appearance: Red powder
Identity: 1H-NMR

Properties

Solvents: DMSO
UV / Vis: 490 nm
Fluorescence: 514 nm

Shipping: AMBIENT
Short Term Storage: +4°C
Long Term Storage: -20°C
Handling Advice: Protect from light and moisture.
Use / Stability: Stable for at least 2 years after receipt when stored at -20°C.

Transportation: Not dangerous goods

Description: 5(6)-Carboxy-2',7'-difluorofluorescein is a fluorinated fluorescein used as a fluorescent dye used as a building block for synthesis of fluorescent probes. It is a replacement for the widely used carboxyfluorescein and fluorescein isothiocyanate since the absorption and emission are virtually unchanged. It can be excited with the 488nm spectral line of the argon-ion laser used in flow cytometers and confocal laser scanning microscopes. The lower pKa, increased resistance to photobleaching, high quantum yield, and diminished quenching on protein conjugation make the dyes superior to other fluoresceins for bioconjugation. Spectral data: Absoprtion 492nm, Emmision 516nm
Smiles: OC(C(F)=C1)=CC2=C1C3(C(C=CC=C4)=C4C(O3)=O)C5=CC(F)=C(O)C=C5O2.CC(O)=O
InChi Key: DDDAACFHCLQORF-UHFFFAOYSA-N
References: (1) W.-C. Sun, et al., J. Org. Chem. 62, 6469 (1997) , (2) N.G. Norager, et al., ACS Chem. Biol. 8, 2033 (2013)
InChi: InChI=1S/C20H10F2O5.CH2O2/c21-13-5-11-17(7-15(13)23)27-18-8-16(24)14(22)6-12(18)19(11)9-3-1-2-4-10(9)20(25)26,2-1-3/h1-8,23H,(H,25,26),1H,(H,2,3)

5(6)-Carboxy-2′,7′-difluorofluorescein is a fluorinated fluorescein used as a fluorescent dye used as a building block for synthesis of fluorescent probes. It is a replacement for the widely used carboxyfluorescein and fluorescein isothiocyanate since the absorption and emission are virtually unchanged. It can be excited with the 488nm spectral line of the argon-ion laser used in flow cytometers and confocal laser scanning microscopes. The lower pKa, increased resistance to photobleaching, high quantum yield, and diminished quenching on protein conjugation make the dyes superior to other fluoresceins for bioconjugation. Spectral data: Absoprtion 492nm, Emmision 516nm

SKU: C0262 Category: Fluorescent Detection

Additional information

Additional information

Purity	≥95% (NMR)
Appearance	Red powder
CAS-Number	251450-64-3
Molecular Formula	C21H12F2O7
Molecular Weight	414.32
Identity	1H-NMR
Solvents	DMSO
UV / Vis	490 nm
Fluorescence	514 nm
Smiles	OC(C(F)=C1)=CC2=C1C3(C(C=CC=C4)=C4C(O3)=O)C5=CC(F)=C(O)C=C5O2.CC(O)=O
InChi Key	DDDAACFHCLQORF-UHFFFAOYSA-N
Shipping	AMBIENT
Short Term Storage	+4°C
Long Term Storage	-20°C
Handling Advice	Protect from light and moisture.
Use / Stability	Stable for at least 2 years after receipt when stored at -20°C.
Transportation	Not dangerous goods
References	(1) W.-C. Sun, et al., J. Org. Chem. 62, 6469 (1997), (2) N.G. Norager, et al., ACS Chem. Biol. 8, 2033 (2013)
InChi	InChI=1S/C20H10F2O5.CH2O2/c21-13-5-11-17(7-15(13)23)27-18-8-16(24)14(22)6-12(18)19(11)9-3-1-2-4-10(9)20(25)26,2-1-3/h1-8,23H,(H,25,26),1H,(H,2,3)
Quantity	50 mg, Bulk