Available from stock

5(6)-FITC

SKU: F0010

Categories: Fluorescent Detection, Fluorescent Detection

Synonyms: Fluorescein 5(6)-isothiocyanate; 27072-45-3
CAS-Number: C₂₁H₁₁NO₅S
Molecular Formula: 389.38
Molecular Weight

Product Data
Handling
Safety Information
Details

Specifications

Purity: ≥95% (HPLC)
Appearance: Yellow to orange powder
Identity: 1H-NMR

Properties

Solvents: aqueous buffers (pH ≥ 6), DMSO
Fluorescence: λex 492 nm, λem 518 nm in 0.1 M phosphate pH 8.0

Documentation

Safety Data Sheet (SDS): CDX F0010 MSDS.pdf

Shipping: AMBIENT
Short Term Storage: +4°C
Long Term Storage: -20°C
Handling Advice: Protect from light and moisture.
Use / Stability: Stable for at least 2 years after receipt when stored at -20°C.

Hazard statements: H334
Precautionary statements: P261, P342 + P311
GHS Symbol: GHS08
Signal word: Danger
Transportation: Not dangerous goods

Description: Fluorescein isothiocyanate (FITC) remains one of the most popular fluorescent labeling reagents. Oligonucleotide conjugates of FITC are frequently employed as hybridization probes. Peptide conjugates of FITC and other fluorescent isothiocyanates are susceptible to Edman degradation, making them useful for high-sensitivity amino acid sequencing, FITC-labeled amino acidd and peptides have been separated by capillary electrophoresis with a detection limit of fewer than 1000 molecules. FITC has also been used to detect proteins in gels and on nitrocellulose membranes and is a selective inhibitor of several membrane ATPases.
Smiles: CN=C=S.OC1=CC2=C(C=C1)C1(OC(=O)C3=C1C=CC=C3)C1=C(O2)C=C(O)C=C1
InChi Key: QRQQVJDEAPQYJW-UHFFFAOYSA-N
References: (1) R.W. Dirks et al.: J. Histochem. Cytochem 38, 467 (1990) , (2) S. Wu et al.: J. Chromatogr 480, 141 (1989) , (3) J.C. Vera et al.: Anal. Biochem 174, 38 (1988) , (4) E.T. Thean et al.: Anal. Biochem 177, 263 (1989) , (5) D.S. Perlin et al.: J. Biol. Chem 259, 9532 (1984) , (6) K.G. Mann et al.: Meth. Enzymol. 26, 28 (1972)
InChi: InChI=1S/C20H12O5.C2H3NS/c21-11-5-7-15-17(9-11)24-18-10-12(22)6-8-16(18)20(15)14-4-2-1-3-13(14)19(23)25-20

Fluorescein isothiocyanate (FITC) remains one of the most popular fluorescent labeling reagents. Oligonucleotide conjugates of FITC are frequently employed as hybridization probes. Peptide conjugates of FITC and other fluorescent isothiocyanates are susceptible to Edman degradation, making them useful for high-sensitivity amino acid sequencing, FITC-labeled amino acidd and peptides have been separated by capillary electrophoresis with a detection limit of fewer than 1000 molecules. FITC has also been used to detect proteins in gels and on nitrocellulose membranes and is a selective inhibitor of several membrane ATPases.

SKU: F0010 Categories: Fluorescent Detection, Fluorescent Detection

Additional information

Additional information

Synonyms	Fluorescein 5(6)-isothiocyanate
Purity	≥95% (HPLC)
Appearance	Yellow to orange powder
CAS-Number	27072-45-3
Molecular Formula	C21H11NO5S
Molecular Weight	389.38
Identity	1H-NMR
Solvents	aqueous buffers (pH ≥ 6), DMSO
Fluorescence	λex 492 nm, λem 518 nm in 0.1 M phosphate pH 8.0
Smiles	CN=C=S.OC1=CC2=C(C=C1)C1(OC(=O)C3=C1C=CC=C3)C1=C(O2)C=C(O)C=C1
InChi Key	QRQQVJDEAPQYJW-UHFFFAOYSA-N
Shipping	AMBIENT
Short Term Storage	+4°C
Long Term Storage	-20°C
Handling Advice	Protect from light and moisture.
Use / Stability	Stable for at least 2 years after receipt when stored at -20°C.
Hazard statements	H334
Precautionary statements	P261, P342 + P311
GHS Symbol	GHS08
Signal word	Danger
Transportation	Not dangerous goods
References	(1) R.W. Dirks et al.: J. Histochem. Cytochem 38, 467 (1990), (2) S. Wu et al.: J. Chromatogr 480, 141 (1989), (3) J.C. Vera et al.: Anal. Biochem 174, 38 (1988), (4) E.T. Thean et al.: Anal. Biochem 177, 263 (1989), (5) D.S. Perlin et al.: J. Biol. Chem 259, 9532 (1984), (6) K.G. Mann et al.: Meth. Enzymol. 26, 28 (1972)
InChi	InChI=1S/C20H12O5.C2H3NS/c21-11-5-7-15-17(9-11)24-18-10-12(22)6-8-16(18)20(15)14-4-2-1-3-13(14)19(23)25-20
Quantity	100 mg, 1 g, 10 g, Bulk