Specifications
- Purity
- ≥90% (HPLC)
- Appearance
- Yellow to orange powder
- Identity
- 1H-NMR
Properties
- Solvents
- DMF, MeOH
Downloads
- Safety Data Sheet
- CDX F0044 MSDS.pdf
Category: Fluorescent Detection, Fluorescent Detection
Amine-reactive fluorescent probe for introducing a fluorescein label at a nucleophilic residue of a target molecule. This fluorescein labeling reagent has an added (X) spacer to improve accessibility of the reactive group by nucleophiles in preparation of bioconjugates. The reactive N-hydroxysuccinimide (NHS) group can be used for easy attachment to proteins or amine containing nucleotides using simple procedures. Spectral properties: λex 490 nm| λem 514 nm in DMF (pH 8.2) and λex 491 nm| ?em 515 nm in 0.1 M Tris pH 9.0.
Synonyms | 5(6)-FAM-X SE, Fluorescein-5(6)-carboxamidocaproic acid N-succinimidyl ester, 6-(Fluorescein-5(6)-carboxamido)hexanoic acid N-succinimidyl ester |
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Purity | ≥90% (HPLC) |
Appearance | Yellow to orange powder |
CAS-Number | 114616-31-8 |
Molecular Formula | C31H26N2O10 |
Molecular Weight | 586.55 |
Identity | 1H-NMR |
Solvents | DMF, MeOH |
Smiles | O=C(C1=C2C=CC=C1)OC32C4=C(C=C(O)C=C4)OC5=CC(O)=CC=C53.CC(NCCCCCC(ON6C(CCC6=O)=O)=O)=O |
InChi Key | AZTAZTWVNFVDPK-UHFFFAOYSA-N |
Shipping | AMBIENT |
Short Term Storage | -20°C |
Long Term Storage | -20°C |
Handling Advice | Protect from light and moisture. |
Use / Stability | Stable for at least 2 years after receipt when stored at -20°C. |
Hazard statements | H315, H319, H335 |
Precautionary statements | P261, P305 + P351 + P338 |
GHS Symbol | GHS07 |
Signal word | Warning |
Transportation | Not dangerous goods |
References | (1) G.P. Jamieson, et al., Cytometry 14, 32 (1993), (2) K.A. Cruickshank, Anal. Biochem. 269, 21 (1999), (3) J.J. MacLean, et al., Biophys. J. 78, 1441 (2000), (4) W.C. Cooper, et al., Biophys. J. 78, 1449 (2000), (5) M.P. Gygi, et al., Nucleic Acids Res. 30, 2790 (2002) |
InChi | InChI=1S/C20H12O5.C12H18N2O5/c21-11-5-7-15-17(9-11)24-18-10-12(22)6-8-16(18)20(15)14-4-2-1-3-13(14)19(23)25-20, 1-9(15)13-8-4-2-3-5-12(18)19-14-10(16)6-7-11(14)17/h1-10,21-22H, 2-8H2,1H3,(H,13,15) |
Quantity | 5 mg, 25 mg, Bulk |