6-SFX SE

SKU: F0048

Category: Fluorescent Detection

Synonyms: 6-FAM-X SE , Fluorescein-6-carboxamidocaproic acid N-succinimidyl ester , 6-(Fluorescein-6-carboxamido)hexanoic acid N-succinimidyl ester; 148356-01-8
CAS-Number: C₃₁H₂₆N₂O₁₀
Molecular Formula: 586.55
Molecular Weight

Product Data
Handling
Safety Information
Details

Specifications

Purity: ≥95% (HPLC)
Identity: 1H-NMR
Appearance: Yellow powder

Properties

Solvents: DMSO, acetone, DMF, methanol

Handling Advice: Protect from light and moisture.
Use / Stability: Stable for at least 2 years after receipt when stored at -20°C.
Long Term Storage: -20°C
Short Term Storage: -20°C
Shipping: AMBIENT

Transportation: Not dangerous goods
Signal word: Warning
GHS Symbol: GHS07
Precautionary statements: P261, P305 + P351 + P338
Hazard statements: H315, H319, H335

Description: Amine-reactive fluorescent probe for introducing a fluorescein label at a nucleophilic residue of a target molecule. This fluorescein labeling reagent has an added (X) spacer to improve accessibility of the reactive group by nucleophiles in preparation of bioconjugates. The reactive N-hydroxysuccinimide (NHS) group can be used for easy attachment to proteins or amine containing nucleotides using simple procedures. Spectral properties, lambdaex 490 nm, lambdaem 514 nm in DMF (pH 8.2) and lambdaex 491 nm, lambdaem 515 nm in 0.1 M Tris pH 9.0.
InChi: InChI=1S/C31H26N2O10/c34-17-8-10-19-23(15-17)42-24-16-18(35)9-11-20(24)28(19)21-5-4-6-22(29(21)31(40)41)30(39)32-14-3-1-2-7-27(38)43-33-25(36)12-13-26(33)37/h4-6,8-11,15-16,34H,1-3,7,12-14H2,(H,32,39)(H,40,41)
References: (1) G.P. Jamieson, et al., Cytometry 14, 32 (1993) , (2) K.A. Cruickshank, Anal. Biochem. 269, 21 (1999) , (3) J.J. MacLean, et al., Biophys. J. 78, 1441 (2000) , (4) W.C. Cooper, et al., Biophys. J. 78, 1449 (2000) , (5) M.P. Gygi, et al., Nucleic Acids Res. 30, 2790 (2002)
InChi Key: LTNYRCVGLBVMPJ-UHFFFAOYSA-N
Smiles: OC1=CC2=C(C(C3=CC=CC(C(NCCCCCC(ON4C(CCC4=O)=O)=O)=O)=C3C(O)=O)=C(C=C5)C(O2)=CC5=O)C=C1

Amine-reactive fluorescent probe for introducing a fluorescein label at a nucleophilic residue of a target molecule. This fluorescein labeling reagent has an added (X) spacer to improve accessibility of the reactive group by nucleophiles in preparation of bioconjugates. The reactive N-hydroxysuccinimide (NHS) group can be used for easy attachment to proteins or amine containing nucleotides using simple procedures. Spectral properties, lambdaex 490 nm, lambdaem 514 nm in DMF (pH 8.2) and lambdaex 491 nm, lambdaem 515 nm in 0.1 M Tris pH 9.0.

SKU: F0048 Category: Fluorescent Detection

Additional information

Additional information

Synonyms	6-FAM-X SE, Fluorescein-6-carboxamidocaproic acid N-succinimidyl ester, 6-(Fluorescein-6-carboxamido)hexanoic acid N-succinimidyl ester
Purity	≥95% (HPLC)
Appearance	Yellow powder
CAS-Number	148356-01-8
Molecular Formula	C31H26N2O10
Molecular Weight	586.55
Identity	1H-NMR
Solvents	DMSO, acetone, DMF, methanol
Smiles	OC1=CC2=C(C(C3=CC=CC(C(NCCCCCC(ON4C(CCC4=O)=O)=O)=O)=C3C(O)=O)=C(C=C5)C(O2)=CC5=O)C=C1
InChi Key	LTNYRCVGLBVMPJ-UHFFFAOYSA-N
Shipping	AMBIENT
Short Term Storage	-20°C
Long Term Storage	-20°C
Handling Advice	Protect from light and moisture.
Use / Stability	Stable for at least 2 years after receipt when stored at -20°C.
Hazard statements	H315, H319, H335
Precautionary statements	P261, P305 + P351 + P338
GHS Symbol	GHS07
Signal word	Warning
Transportation	Not dangerous goods
References	(1) G.P. Jamieson, et al., Cytometry 14, 32 (1993), (2) K.A. Cruickshank, Anal. Biochem. 269, 21 (1999), (3) J.J. MacLean, et al., Biophys. J. 78, 1441 (2000), (4) W.C. Cooper, et al., Biophys. J. 78, 1449 (2000), (5) M.P. Gygi, et al., Nucleic Acids Res. 30, 2790 (2002)
InChi	InChI=1S/C31H26N2O10/c34-17-8-10-19-23(15-17)42-24-16-18(35)9-11-20(24)28(19)21-5-4-6-22(29(21)31(40)41)30(39)32-14-3-1-2-7-27(38)43-33-25(36)12-13-26(33)37/h4-6,8-11,15-16,34H,1-3,7,12-14H2,(H,32,39)(H,40,41)
Quantity	10 mg, 25 mg, Bulk