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    6-SFX SE

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      6-SFX SE

      SKU
      F0048

      Categories: Fluorescent Detection, Fluorescent Detection

      Synonyms
      6-FAM-X SE , Fluorescein-6-carboxamidocaproic acid N-succinimidyl ester , 6-(Fluorescein-6-carboxamido)hexanoic acid N-succinimidyl ester
      148356-01-8
      CAS-Number
      C31H26N2O10
      Molecular Formula
      586.55
      Molecular Weight

      • Product Data
      • Handling
      • Safety Information
      • Details

      Specifications

      Purity
      ≥95% (HPLC)
      Identity
      1H-NMR
      Appearance
      Yellow powder

      Properties

      Solvents
      DMSO, acetone, DMF, methanol
      Handling Advice
      Protect from light and moisture.
      Use / Stability
      Stable for at least 2 years after receipt when stored at -20°C.
      Long Term Storage
      -20°C
      Short Term Storage
      -20°C
      Shipping
      AMBIENT
      Transportation
      Not dangerous goods
      Signal word
      Warning
      GHS Symbol
      GHS07
      Precautionary statements
      P261, P305 + P351 + P338
      Hazard statements
      H315, H319, H335
      Description
      Amine-reactive fluorescent probe for introducing a fluorescein label at a nucleophilic residue of a target molecule. This fluorescein labeling reagent has an added (X) spacer to improve accessibility of the reactive group by nucleophiles in preparation of bioconjugates. The reactive N-hydroxysuccinimide (NHS) group can be used for easy attachment to proteins or amine containing nucleotides using simple procedures. Spectral properties, lambdaex 490 nm, lambdaem 514 nm in DMF (pH 8.2) and lambdaex 491 nm, lambdaem 515 nm in 0.1 M Tris pH 9.0.
      InChi
      InChI=1S/C31H26N2O10/c34-17-8-10-19-23(15-17)42-24-16-18(35)9-11-20(24)28(19)21-5-4-6-22(29(21)31(40)41)30(39)32-14-3-1-2-7-27(38)43-33-25(36)12-13-26(33)37/h4-6,8-11,15-16,34H,1-3,7,12-14H2,(H,32,39)(H,40,41)
      References
      (1) G.P. Jamieson, et al., Cytometry 14, 32 (1993) , (2) K.A. Cruickshank, Anal. Biochem. 269, 21 (1999) , (3) J.J. MacLean, et al., Biophys. J. 78, 1441 (2000) , (4) W.C. Cooper, et al., Biophys. J. 78, 1449 (2000) , (5) M.P. Gygi, et al., Nucleic Acids Res. 30, 2790 (2002)
      InChi Key
      LTNYRCVGLBVMPJ-UHFFFAOYSA-N
      Smiles
      OC1=CC2=C(C(C3=CC=CC(C(NCCCCCC(ON4C(CCC4=O)=O)=O)=O)=C3C(O)=O)=C(C=C5)C(O2)=CC5=O)C=C1

      Amine-reactive fluorescent probe for introducing a fluorescein label at a nucleophilic residue of a target molecule. This fluorescein labeling reagent has an added (X) spacer to improve accessibility of the reactive group by nucleophiles in preparation of bioconjugates. The reactive N-hydroxysuccinimide (NHS) group can be used for easy attachment to proteins or amine containing nucleotides using simple procedures. Spectral properties, lambdaex 490 nm, lambdaem 514 nm in DMF (pH 8.2) and lambdaex 491 nm, lambdaem 515 nm in 0.1 M Tris pH 9.0.

      SKU: F0048 Categories: Fluorescent Detection, Fluorescent Detection
      • Additional information

      Additional information

      Synonyms

      6-FAM-X SE, Fluorescein-6-carboxamidocaproic acid N-succinimidyl ester, 6-(Fluorescein-6-carboxamido)hexanoic acid N-succinimidyl ester

      Purity

      ≥95% (HPLC)

      Appearance

      Yellow powder

      CAS-Number

      148356-01-8

      Molecular Formula

      C31H26N2O10

      Molecular Weight

      586.55

      Identity

      1H-NMR

      Solvents

      DMSO, acetone, DMF, methanol

      Smiles

      OC1=CC2=C(C(C3=CC=CC(C(NCCCCCC(ON4C(CCC4=O)=O)=O)=O)=C3C(O)=O)=C(C=C5)C(O2)=CC5=O)C=C1

      InChi Key

      LTNYRCVGLBVMPJ-UHFFFAOYSA-N

      Shipping

      AMBIENT

      Short Term Storage

      -20°C

      Long Term Storage

      -20°C

      Handling Advice

      Protect from light and moisture.

      Use / Stability

      Stable for at least 2 years after receipt when stored at -20°C.

      Hazard statements

      H315, H319, H335

      Precautionary statements

      P261, P305 + P351 + P338

      GHS Symbol

      GHS07

      Signal word

      Warning

      Transportation

      Not dangerous goods

      References

      (1) G.P. Jamieson, et al., Cytometry 14, 32 (1993), (2) K.A. Cruickshank, Anal. Biochem. 269, 21 (1999), (3) J.J. MacLean, et al., Biophys. J. 78, 1441 (2000), (4) W.C. Cooper, et al., Biophys. J. 78, 1449 (2000), (5) M.P. Gygi, et al., Nucleic Acids Res. 30, 2790 (2002)

      InChi

      InChI=1S/C31H26N2O10/c34-17-8-10-19-23(15-17)42-24-16-18(35)9-11-20(24)28(19)21-5-4-6-22(29(21)31(40)41)30(39)32-14-3-1-2-7-27(38)43-33-25(36)12-13-26(33)37/h4-6,8-11,15-16,34H,1-3,7,12-14H2,(H,32,39)(H,40,41)

      Quantity

      10 mg, 25 mg, Bulk

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