Specifications
- Purity
- ≥99% (Titr.)
- Appearance
- White to off-white powder
- Identity
- 1H-NMR
Properties
- Solvents
- Soluble in methanol or chloroform.
- Melting Point
- 69.0 - 75.0 °C
Available from stock
7-Diethylamino-4-methylcoumarin
- SKU
- D0124
Category: Building Blocks, Intermediates, Reagents
- Synonyms
- Coumarin 1, Coumarin 460, Coumarin 47, 7D4MC, C47, C460, DEMC, C.I. 551100, C.I. Fluorescent Brightener 140, C.I. Fluorescent Brightener 52, Hakkol P, Neo-Super HR 1, NSC 61830
- 91-44-1
- CAS-Number
- C14H17NO2
- Molecular Formula
- 231.29
- Molecular Weight
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
- Shipping
- AMBIENT
- Short Term Storage
- RT
- Long Term Storage
- RT
- Handling Advice
- Protect from light and moisture.
- Use / Stability
- Stable for at least 2 years after receipt when stored at RT.
- Transportation
- Not dangerous goods
- Description
- 3,3'-Diethyloxacarbocyanine iodide is a cyanine based dye that has been used for measuring membrane potentials in bacteria. The green fluorescent dye forms red fluorescent aggregates with increasing membrane potential. The spectral shifting phenomenon has been exploited to measure membrane potential and analyze bacterial viability by flow cytometry ratiometrically. Spectral data: λmax 483nm (methanol); λem 498nm (methanol). The dye has also been used as laser dye and in photosensitization of mitochondria and endoplastic recticulum in animals and plant cells. It may be used as a conjugate for labelling cells for cytofluorometry studies and in the imaging of carbon nanotubes(CNTs) using fluorescence microscopy.
- Smiles
- CC(C1=CC=C(N(CC)CC)C=C1O2)=CC2=O
- InChi Key
- AFYCEAFSNDLKSX-UHFFFAOYSA-N
- References
- (1) D.N. Dempster, et al.; J. Chem. Soc. Faraday II 68, 1479 (1972) , (2) D. Magde & M.W. Windsor; Chem. Phys. Lett. 27, 31 (1974) , (3) M. Zalokar & C. Sardet; Dev. Biol. 102, 195 (1984) , (4) M. Castedo, et al.; J. Immunol. Meth. 265, 39 (2002) , (5) H.J. Broxterman, et al.; Leukemia 11, 1110 (1997) , (6) D.J. Novo, et al.; Cytometry 35, 55 (1999) , (7) D.J. Novo, et al.; Antimicrob. Agents Chemother. 44, 827 (2000) , (8) D.R. Gentry, et al.; J. Microbiol. Methods 83, 254 (2010) , (9) R.W. Sabnis; Handbook of biological dyes and stains (2010) , (10) M.A. Hudson, et al.; Antimicrob. Agents Chemother. 64, e00910 (2020)
3,3′-Diethyloxacarbocyanine iodide is a cyanine based dye that has been used for measuring membrane potentials in bacteria. The green fluorescent dye forms red fluorescent aggregates with increasing membrane potential. The spectral shifting phenomenon has been exploited to measure membrane potential and analyze bacterial viability by flow cytometry ratiometrically. Spectral data: λmax 483nm (methanol); λem 498nm (methanol). The dye has also been used as laser dye and in photosensitization of mitochondria and endoplastic recticulum in animals and plant cells. It may be used as a conjugate for labelling cells for cytofluorometry studies and in the imaging of carbon nanotubes(CNTs) using fluorescence microscopy.
Additional information
| Synonyms | Coumarin 1, Coumarin 460, Coumarin 47, 7D4MC, C47, C460, DEMC, C.I. 551100, C.I. Fluorescent Brightener 140, C.I. Fluorescent Brightener 52, Hakkol P, Neo-Super HR 1, NSC 61830 |
|---|---|
| Purity | ≥99% (Titr.) |
| Appearance | White to off-white powder |
| CAS-Number | 91-44-1 |
| Molecular Formula | C14H17NO2 |
| Molecular Weight | 231.29 |
| Identity | 1H-NMR |
| Solvents | Soluble in methanol or chloroform. |
| Melting Point | 69.0 – 75.0 °C |
| Smiles | CC(C1=CC=C(N(CC)CC)C=C1O2)=CC2=O |
| InChi Key | AFYCEAFSNDLKSX-UHFFFAOYSA-N |
| Shipping | AMBIENT |
| Short Term Storage | RT |
| Long Term Storage | RT |
| Handling Advice | Protect from light and moisture. |
| Use / Stability | Stable for at least 2 years after receipt when stored at RT. |
| Transportation | Not dangerous goods |
| References | (1) D.N. Dempster, et al.; J. Chem. Soc. Faraday II 68, 1479 (1972), (2) D. Magde & M.W. Windsor; Chem. Phys. Lett. 27, 31 (1974), (3) M. Zalokar & C. Sardet; Dev. Biol. 102, 195 (1984), (4) M. Castedo, et al.; J. Immunol. Meth. 265, 39 (2002), (5) H.J. Broxterman, et al.; Leukemia 11, 1110 (1997), (6) D.J. Novo, et al.; Cytometry 35, 55 (1999), (7) D.J. Novo, et al.; Antimicrob. Agents Chemother. 44, 827 (2000), (8) D.R. Gentry, et al.; J. Microbiol. Methods 83, 254 (2010), (9) R.W. Sabnis; Handbook of biological dyes and stains (2010), (10) M.A. Hudson, et al.; Antimicrob. Agents Chemother. 64, e00910 (2020) |
| Quantity | 100 g, Bulk |
