Specifications
- Purity
- ≥98% (NMR)
- Appearance
- Beige powder
- Identity
- 1H-NMR
Properties
- Solvents
- DMSO, methanol
- Melting Point
- 212 °C (dec.) (lit.)
Available from stock
7-Hydroxy-4-coumarinylacetic acid
- SKU
- H0008
Categories: Fluorescent Detection, Fluorescent Detection
- Synonyms
- 7-HCA , 7-Hydroxycoumarin-4-acetic acid , Umbelliferone-4-acetic acid , NSC 642907 , NSC 65625
- 6950-82-9
- CAS-Number
- C11H8O5
- Molecular Formula
- 220.18
- Molecular Weight
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
- Shipping
- AMBIENT
- Short Term Storage
- +4°C
- Long Term Storage
- -20°C
- Handling Advice
- Protect from light and moisture.
- Use / Stability
- Stable for at least 2 years after receipt when stored at -20°C.
- Hazard statements
- H315-H319-H335
- Precautionary statements
- P261-P305 + P351 + P338
- GHS Symbol
- GHS07
- Signal word
- Warning
- Transportation
- Not dangerous goods
- Description
- 7-Hydroxycoumarin-4-acetic acid is a fluorescent pH indicator. 7-Hydroxycoumarin-4-acetic acid is used for labeling amine-specific peptides, proteins and other biological molecules to give blue fluorescence. It is used as fluorigenic substrate, e.g. in BSA-HCA complexes for fatty acid concentration measurement or for fluorometric enzymatic analysis. It also can be used as a building block for synthesis of other coumarin-based probes. Spectral data: Ex=360nm, Em=450nm (DMSO).
- Smiles
- OC1=CC=C2C(OC(C=C2CC(O)=O)=O)=C1
- InChi Key
- BNHPMQBVNXMPDU-UHFFFAOYSA-N
- References
- (1) G. Gabor & D.R. Walt, Anal. Chem. 63, 793 (1991) , (2) E.J.F. Demant, BBA 1304, 43 (1996) , (3) J.F. Sierra, et al., Anal. Chim. Acta 368, 97 (1998) , (4) E.J.F. Demant, Anal. Biochem. 267, 366 (1999) , (5) C. Charitos, et al., J. Heterocyclic Chem. 38, 153 (2001) , (6) K.F. Chilvers, et al., J. Appl. Microbiol. 91, 1118 (2001) , (7) S. Chen, et al., Anal. Meth. 7, 7646 (2015) , (8) L. Rong, et al., Chem. Commun. 50, 667 (2014)
- InChi
- InChI=1S/C11H8O5/c12-7-1-2-8-6(3-10(13)14)4-11(15)16-9(8)5-7/h1-2,4-5,12H,3H2,(H,13,14)
7-Hydroxycoumarin-4-acetic acid is a fluorescent pH indicator. 7-Hydroxycoumarin-4-acetic acid is used for labeling amine-specific peptides, proteins and other biological molecules to give blue fluorescence. It is used as fluorigenic substrate, e.g. in BSA-HCA complexes for fatty acid concentration measurement or for fluorometric enzymatic analysis. It also can be used as a building block for synthesis of other coumarin-based probes. Spectral data: Ex=360nm, Em=450nm (DMSO).
Additional information
| Synonyms | 7-HCA, 7-Hydroxycoumarin-4-acetic acid, Umbelliferone-4-acetic acid, NSC 642907, NSC 65625 |
|---|---|
| Purity | ≥98% (NMR) |
| Appearance | Beige powder |
| CAS-Number | 6950-82-9 |
| Molecular Formula | C11H8O5 |
| Molecular Weight | 220.18 |
| Identity | 1H-NMR |
| Solvents | DMSO, methanol |
| Melting Point | 212 °C (dec.) (lit.) |
| Smiles | OC1=CC=C2C(OC(C=C2CC(O)=O)=O)=C1 |
| InChi Key | BNHPMQBVNXMPDU-UHFFFAOYSA-N |
| Shipping | AMBIENT |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice | Protect from light and moisture. |
| Use / Stability | Stable for at least 2 years after receipt when stored at -20°C. |
| Hazard statements | H315-H319-H335 |
| Precautionary statements | P261-P305 + P351 + P338 |
| GHS Symbol | GHS07 |
| Signal word | Warning |
| Transportation | Not dangerous goods |
| References | (1) G. Gabor & D.R. Walt, Anal. Chem. 63, 793 (1991), (2) E.J.F. Demant, BBA 1304, 43 (1996), (3) J.F. Sierra, et al., Anal. Chim. Acta 368, 97 (1998), (4) E.J.F. Demant, Anal. Biochem. 267, 366 (1999), (5) C. Charitos, et al., J. Heterocyclic Chem. 38, 153 (2001), (6) K.F. Chilvers, et al., J. Appl. Microbiol. 91, 1118 (2001), (7) S. Chen, et al., Anal. Meth. 7, 7646 (2015), (8) L. Rong, et al., Chem. Commun. 50, 667 (2014) |
| InChi | InChI=1S/C11H8O5/c12-7-1-2-8-6(3-10(13)14)4-11(15)16-9(8)5-7/h1-2,4-5,12H,3H2,(H,13,14) |
| Quantity | 5 g, Bulk |
