Specifications
- Purity
- ≥98% (TLC)
- Appearance
- Beige crystalline powder
- Identity
- 1H-NMR
Properties
- Solvents
- DMF, DMSO, methanol
- Melting Point
- 261 °C (lit.)
Available from stock
7-Hydroxycoumarin-3-carboxylic acid
- SKU
- H0015
Categories: Fluorescent Detection, Fluorescent Detection
- Synonyms
- Umbelliferone-3-carboxylic acid , HCC , NSC 115545 , 7-OHCCA
- 779-27-1
- CAS-Number
- C10H6O5
- Molecular Formula
- 206.15
- Molecular Weight
- Shipping
- AMBIENT
- Short Term Storage
- +4°C
- Long Term Storage
- -20°C
- Handling Advice
- Protect from light and moisture.
- Use / Stability
- Stable for at least 2 years after receipt when stored at -20°C.
- Hazard statements
- H315-H319-H335
- Precautionary statements
- P261-P305 + P351 + P338
- GHS Symbol
- GHS07
- Signal word
- Warning
- Transportation
- Not dangerous goods
- Description
- 7-Hydroxycoumarin-3-carboxylic acid is one of the most popular blue fluorophores for labeling proteins and nucleic acids mostly through the in situ formation of its succinimidyl ester. It is also used to label peptides, nucleotides and carbohydrates. This coumarin is used as a fluorogenic substrate for glycosyltransferase assays and as a pH-indicator. Spectral data: Ex/Em = 386/448 nm (TRIS, pH9). Shown to have cytotoxic activity in some cancer cells.
- Smiles
- OC1=CC=C2C(OC(C(C(O)=O)=C2)=O)=C1
- InChi Key
- LKLWLDOUZJEHDY-UHFFFAOYSA-N
- References
- (1) H. Offenbacher, et al., Sens. Actuators 9, 73 (1986) , (2) H. Li, et al., J. Cell Biol. 134, 1019 (1996) , (3) K. Higai, et al., Biol. Pharm. Bull. 22, 333 (1999) , (4) B. Thati, et al., Cancer Lett. 248, 321 (2007) , (5) R.W. Sabnis, Handbook of Fluorescent Dyes and Probes p246 (2015)
7-Hydroxycoumarin-3-carboxylic acid is one of the most popular blue fluorophores for labeling proteins and nucleic acids mostly through the in situ formation of its succinimidyl ester. It is also used to label peptides, nucleotides and carbohydrates. This coumarin is used as a fluorogenic substrate for glycosyltransferase assays and as a pH-indicator. Spectral data: Ex/Em = 386/448 nm (TRIS, pH9). Shown to have cytotoxic activity in some cancer cells.
Additional information
| Synonyms | Umbelliferone-3-carboxylic acid, HCC, NSC 115545, 7-OHCCA |
|---|---|
| Purity | ≥98% (TLC) |
| Appearance | Beige crystalline powder |
| CAS-Number | 779-27-1 |
| Molecular Formula | C10H6O5 |
| Molecular Weight | 206.15 |
| Identity | 1H-NMR |
| Solvents | DMF, DMSO, methanol |
| Melting Point | 261 °C (lit.) |
| Smiles | OC1=CC=C2C(OC(C(C(O)=O)=C2)=O)=C1 |
| InChi Key | LKLWLDOUZJEHDY-UHFFFAOYSA-N |
| Shipping | AMBIENT |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice | Protect from light and moisture. |
| Use / Stability | Stable for at least 2 years after receipt when stored at -20°C. |
| Hazard statements | H315-H319-H335 |
| Precautionary statements | P261-P305 + P351 + P338 |
| GHS Symbol | GHS07 |
| Signal word | Warning |
| Transportation | Not dangerous goods |
| References | (1) H. Offenbacher, et al., Sens. Actuators 9, 73 (1986), (2) H. Li, et al., J. Cell Biol. 134, 1019 (1996), (3) K. Higai, et al., Biol. Pharm. Bull. 22, 333 (1999), (4) B. Thati, et al., Cancer Lett. 248, 321 (2007), (5) R.W. Sabnis, Handbook of Fluorescent Dyes and Probes p246 (2015) |
| Quantity | 100 mg, 1 g, Bulk |
