Available from stock

8-Aminopyrene-1,3,6-trisulfonic acid trisodium salt

SKU: A0097

Category: Fluorescent Detection

Synonyms: APTS, 1,3,6-Pyrenetrisulfonic acid, 8-amino trisodium salt, Trisodium 8-aminopyrene-1,3,6-trisulfonate; 196504-57-1
CAS-Number: C₁₆H₈NNa₃O₉S₃
Molecular Formula: 523.40
Molecular Weight

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Product Data
Handling
Safety Information
Details

Specifications

Purity: ≥96% (HPLC)
Appearance: Yellow to dark yellow powder
Identity: 1H-NMR

Properties

Solvents: Soluble in water (10mg/ml), DMF or DMSO (1mg/ml).
Melting Point: ≥250 °C (lit.)

Shipping: AMBIENT
Short Term Storage: +4°C
Long Term Storage: -20°C
Handling Advice: Protect from light and moisture.
Use / Stability: Stable for at least 2 years after receipt when stored at -20°C.

Hazard statements: H315,H319,H335
Precautionary statements: P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338
GHS Symbol: GHS07
Signal word: Warning
Transportation: Not dangerous goods

Description: 8-Aminopyrene-1,3,6-trisulfonic acid (APTS) is an anionic fluorescent dye. It forms a ground state complex with viologen-type quenchers, inducing a red-shift in the absorbance spectrum from 425 to 464 nm. APTS is pH insensitive, with the emission maximum remaining constant over the pH range of 4-10. APTS is a highly fluorescent labeling reagent widely used for glycan and carbohydrate analysis. Through reductive amination, APTS efficiently labels reducing sugars, enabling sensitive detection and high-resolution separation by capillary electrophoresis with laser-induced fluorescence (CE-LIF). Spectral Data: Ex/Em maxima 425/503 nm (in pH 7.4 PBS).
Smiles: [Na+].[Na+].[Na+].Nc1cc(c2ccc3c(cc(c4ccc1c2c34)S([O-])(=O)=O)S([O-])(=O)=O)S([O-])(=O)=O
InChi Key: XSTNYACEWLNWPY-UHFFFAOYSA-K
References: (1) R.A. Evangelista, et al., Electrophoresis 17, 347 (1996) , (2) Y. Yoshinaka, et al., J.Chromatogr. 1143, 83 (2007) , (3) Z. Sharrett, et al., Org. Biomol. Chem. 7, 1461 (2009) , (4) P. Smejkal, et al., Electrophoresis 31, 3783 (2010) , (5) D.B. Cordes & B. Singaram, Pure Appl. chem. 84, 2183 (2012) , (6) S.C. Bunz, et al., Anal. Bioanal. Chem. 405, 8277 (2013) , (7) H.T. Feng, et al., Electrophoresis 38, 1788 (2017) , (8) S. Yamamoto, et al., J. Chromatogr. A. 1566, 44 (2018) , (9) J. Krenkova, et al., Electrophoresis 42, 1333 (2021) , (10) Y. Deng, et al., J. Pharm. Anal. 13, 201 (2023) , (11) J.M. Garber, et al., Methods Mol. Biol. 2657, 223 (2023)
InChi: InChI=1S/C16H11NO9S3.3Na/c17-11-5-12(27(18,19)20)8-3-4-10-14(29(24,25)26)6-13(28(21,22)23)9-2-1-7(11)15(8)16(9)10,,,/h1-6H,17H2,(H,18,19,20)(H,21,22,23)(H,24,25,26),,,/q,3*+1/p-3

8-Aminopyrene-1,3,6-trisulfonic acid (APTS) is an anionic fluorescent dye. It forms a ground state complex with viologen-type quenchers, inducing a red-shift in the absorbance spectrum from 425 to 464 nm. APTS is pH insensitive, with the emission maximum remaining constant over the pH range of 4-10. APTS is a highly fluorescent labeling reagent widely used for glycan and carbohydrate analysis. Through reductive amination, APTS efficiently labels reducing sugars, enabling sensitive detection and high-resolution separation by capillary electrophoresis with laser-induced fluorescence (CE-LIF). Spectral Data: Ex/Em maxima 425/503 nm (in pH 7.4 PBS).

SKU: A0097 Category: Fluorescent Detection

Additional information

Additional information

Synonyms	APTS, 1,3,6-Pyrenetrisulfonic acid, 8-amino trisodium salt, Trisodium 8-aminopyrene-1,3,6-trisulfonate
Purity	≥96% (HPLC)
Appearance	Yellow to dark yellow powder
CAS-Number	196504-57-1
Molecular Formula	C16H8NNa3O9S3
Molecular Weight	523.40
Identity	1H-NMR
Solvents	Soluble in water (10mg/ml), DMF or DMSO (1mg/ml).
Melting Point	≥250 °C (lit.)
Smiles	[Na+].[Na+].[Na+].Nc1cc(c2ccc3c(cc(c4ccc1c2c34)S([O-])(=O)=O)S([O-])(=O)=O)S([O-])(=O)=O
InChi Key	XSTNYACEWLNWPY-UHFFFAOYSA-K
Shipping	AMBIENT
Short Term Storage	+4°C
Long Term Storage	-20°C
Handling Advice	Protect from light and moisture.
Use / Stability	Stable for at least 2 years after receipt when stored at -20°C.
Hazard statements	H315,H319,H335
Precautionary statements	P261 – P264 – P271 – P280 – P302 + P352 – P305 + P351 + P338
GHS Symbol	GHS07
Signal word	Warning
Transportation	Not dangerous goods
References	(1) R.A. Evangelista, et al., Electrophoresis 17, 347 (1996), (2) Y. Yoshinaka, et al., J.Chromatogr. 1143, 83 (2007), (3) Z. Sharrett, et al., Org. Biomol. Chem. 7, 1461 (2009), (4) P. Smejkal, et al., Electrophoresis 31, 3783 (2010), (5) D.B. Cordes & B. Singaram, Pure Appl. chem. 84, 2183 (2012), (6) S.C. Bunz, et al., Anal. Bioanal. Chem. 405, 8277 (2013), (7) H.T. Feng, et al., Electrophoresis 38, 1788 (2017), (8) S. Yamamoto, et al., J. Chromatogr. A. 1566, 44 (2018), (9) J. Krenkova, et al., Electrophoresis 42, 1333 (2021), (10) Y. Deng, et al., J. Pharm. Anal. 13, 201 (2023), (11) J.M. Garber, et al., Methods Mol. Biol. 2657, 223 (2023)
InChi	InChI=1S/C16H11NO9S3.3Na/c17-11-5-12(27(18,19)20)8-3-4-10-14(29(24,25)26)6-13(28(21,22)23)9-2-1-7(11)15(8)16(9)10,,,/h1-6H,17H2,(H,18,19,20)(H,21,22,23)(H,24,25,26),,,/q,3*+1/p-3
Quantity	5 mg, 25 mg, Bulk