Available from stock

8-Anilino-1-naphthalenesulfonic acid

SKU: A0112

Category: Fluorescent Detection

Synonyms: N-Phenyl peri acid, 1,8-ANS, ANS, ANSA, 8-Anilino-1-naphthalenesulfonate, 1-Anilino-8-naphthalenesulfonic acid, NSC 1746; 82-76-8
CAS-Number: C₁₆H₁₃NO₃S
Molecular Formula: 299.34
Molecular Weight

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Product Data
Handling
Safety Information
Details

Specifications

Purity: ≥97% (HPLC)
Appearance: Gray to dark green to black powder
Identity: 1H-NMR

Properties

Solvents: Soluble in DMSO (20mg/ml), DMF (50 mg/ml) or methanol (20mg/ml). Slightly soluble in water (<1mg/ml).
Melting Point: 215-217 °C (dec.) (lit.)

Documentation

Safety Data Sheet (SDS): CDX A0112 SDS Vs1.1.pdf

Shipping: AMBIENT
Short Term Storage: RT
Long Term Storage: RT
Handling Advice: Protect from light and moisture.
Use / Stability: Stable for at least 2 years after receipt when stored at RT.

Transportation: Not dangerous goods

Description: 8-Anilino-1-naphthalenesulfonic acid (ANS) is a widely used environment-sensitive fluorescent probe for studying protein structure, conformational changes, and hydrophobic surface exposure. ANS exhibits weak fluorescence in aqueous solutions but shows a strong increase in fluorescence intensity and a blue shift in emission upon binding to hydrophobic regions of proteins, membranes, or macromolecular complexes. This property makes ANS an essential tool for protein folding/unfolding studies, ligand-binding assays, and investigations of protein–protein interactions. It is commonly applied in biochemical and biophysical research to monitor changes in protein conformation under varying conditions such as pH, temperature, or denaturant concentration. Spectral data: ?ex 371 nm, ?em 480 nm in methanol.
Smiles: OS(=O)(=O)c1cccc2cccc(Nc3ccccc3)c12
InChi Key: FWEOQOXTVHGIFQ-UHFFFAOYSA-N
References: [1] N. Gains & A.P. Dawson AP, Biochem. J. 148, 157 (1975) , [2] V. Cody & J. Hazel, BBRC 68, 425 (1976) , [3] X.C. Yu & H.W. Strobel, Mol. Cell Biochem. 162, 89 (1996) , [4] J. Ishida, et al., Biol. Pharm. Bull. 19, 1391 (1996) , [5] J. Malecki & Z. Wasylewski, J. Protein Chem. 17, 745 (1998) , [6] M. Andujar-Sanchez, et al., J. Mol. Recognit. 24, 548 (2011) , [7] H.K. Vivek, et al., Anal. Biochem. 461, 27 (2014) , [8] C. Ota & K. Takano, Chemphyschem. 20, 1456 (2019) , [9] E.B. Faber, et al., ACS Chem. Biol. 15, 1759 (2020)

8-Anilino-1-naphthalenesulfonic acid (ANS) is a widely used environment-sensitive fluorescent probe for studying protein structure, conformational changes, and hydrophobic surface exposure. ANS exhibits weak fluorescence in aqueous solutions but shows a strong increase in fluorescence intensity and a blue shift in emission upon binding to hydrophobic regions of proteins, membranes, or macromolecular complexes. This property makes ANS an essential tool for protein folding/unfolding studies, ligand-binding assays, and investigations of protein–protein interactions. It is commonly applied in biochemical and biophysical research to monitor changes in protein conformation under varying conditions such as pH, temperature, or denaturant concentration. Spectral data: ?ex 371 nm, ?em 480 nm in methanol.

SKU: A0112 Category: Fluorescent Detection

Additional information

Additional information

Synonyms	N-Phenyl peri acid, 1,8-ANS, ANS, ANSA, 8-Anilino-1-naphthalenesulfonate, 1-Anilino-8-naphthalenesulfonic acid, NSC 1746
Purity	≥97% (HPLC)
Appearance	Gray to dark green to black powder
CAS-Number	82-76-8
Molecular Formula	C16H13NO3S
Molecular Weight	299.34
Identity	1H-NMR
Solvents	Soluble in DMSO (20mg/ml), DMF (50 mg/ml) or methanol (20mg/ml). Slightly soluble in water (<1mg/ml).
Melting Point	215-217 °C (dec.) (lit.)
Smiles	OS(=O)(=O)c1cccc2cccc(Nc3ccccc3)c12
InChi Key	FWEOQOXTVHGIFQ-UHFFFAOYSA-N
Shipping	AMBIENT
Short Term Storage	RT
Long Term Storage	RT
Handling Advice	Protect from light and moisture.
Use / Stability	Stable for at least 2 years after receipt when stored at RT.
Transportation	Not dangerous goods
References	[1] N. Gains & A.P. Dawson AP, Biochem. J. 148, 157 (1975), [2] V. Cody & J. Hazel, BBRC 68, 425 (1976) , [3] X.C. Yu & H.W. Strobel, Mol. Cell Biochem. 162, 89 (1996) , [4] J. Ishida, et al., Biol. Pharm. Bull. 19, 1391 (1996) , [5] J. Malecki & Z. Wasylewski, J. Protein Chem. 17, 745 (1998) , [6] M. Andujar-Sanchez, et al., J. Mol. Recognit. 24, 548 (2011) , [7] H.K. Vivek, et al., Anal. Biochem. 461, 27 (2014) , [8] C. Ota & K. Takano, Chemphyschem. 20, 1456 (2019) , [9] E.B. Faber, et al., ACS Chem. Biol. 15, 1759 (2020)
Quantity	25 g, 100 g, Bulk