Specifications
- Purity
- ≥98% (HPLC)
- Appearance
- White to off-white powder
- Identity
- 1H-NMR
Properties
- Solvents
- DMSO, DMF (slightly)
- Melting Point
- 220-230 °C (dec.)
Biotin-X is an amino-reactive biotinylation reagent with a long spacer arm. The 6-aminocaproic acid spacer arm provides distance between the biotin and the target ligand which helps alleviate steric hindrance. Biotin-X can be used to biotinylate antibodies, proteins, peptides, or amino acids by reacting with primary amines as found in the N-terminus and Lysine side chains. Biotin-X may also be used in the biosynthesis of biotinylated oligosaccharides. Optimal reaction conditions are at a pH of 7-9. Avoid amine-containing buffers, which may compete in the acylation reaction.
Synonyms | N-(+)-Biotinyl-6-aminohexanoic acid, Biotin caproic acid, (+)-Biotin-epsilon-aminocaproic acid, 6-((Biotinoyl)amino)hexanoic acid, N-(+)-Biotinyl-6-aminocaproic acid |
---|---|
Purity | ≥98% (HPLC) |
Appearance | White to off-white powder |
CAS-Number | 72040-64-3 |
Molecular Formula | C16H27N3O4S |
Molecular Weight | 357.47 |
Identity | 1H-NMR |
Solvents | DMSO, DMF (slightly) |
Melting Point | 220-230 °C (dec.) |
InChi Key | CMUGHZFPFWNUQT-HUBLWGQQSA-N |
Shipping | AMBIENT |
Short Term Storage | +4°C |
Long Term Storage | -20°C |
Handling Advice | Protect from light and moisture. |
Use / Stability | Stable for at least 2 years after receipt when stored at -20°C. |
Transportation | Not dangerous goods |
References | (1) G.T. Hermanson, Bioconjugate Techn. 2nd p506-545 (2008) |
Quantity | 100 mg, 500 mg, Bulk |