5(6)-CFDA N-succinimidyl ester
- SKU
- C0037
Category: Fluorescent Detection, Fluorescent Detection
- Synonyms
- 5(6)-Carboxyfluorescein diacetate N-succinimidyl ester , CFSE
- 150347-59-4
- CAS-Number
- C29H19NO11
- Molecular Formula
- 557.46
- Molecular Weight
Specifications
- Purity
- ≥90% (HPLC)
- Appearance
- White or slightly yellow powder
- Identity
- 1H-NMR
Properties
- Solvents
- DMSO, DMF, chloroform
- Melting Point
- 152-154 °C (lit.)
- Fluorescence
- λex 492 nm, λem 517 nm in 0.1 M Tris pH 8.0
Downloads
- Safety Data Sheet
- CDX C0037 MSDS.pdf
- Shipping
- AMBIENT
- Short Term Storage
- +4°C
- Long Term Storage
- -20°C
- Handling Advice
- Protect from light and moisture.
- Use / Stability
- Stable for at least 2 years after receipt when stored at -20°C.
- Transportation
- Not dangerous goods
- Description
- 5(6)-Carboxyfluorescein diacetate N-succinimidyl ester is a useful fluorescent tracer that can passivley diffuse into cells and covalently label intracellular proteins, resulting in long-term cell labeling. The reagent itself is colorless and nonfluorescent but becomes brightly green fluorescent once it is hydrolyzed by intracellular esterases.
- Smiles
- CC(=O)ON1C(=O)CCC1=O.CC(=O)OC1=CC2=C(C=C1)C1(OC(=O)C3=C1C=CC=C3)C1=CC=C(OC(C)=O)C=C1O2
- InChi Key
- KQPBJOQXHVRGEG-UHFFFAOYSA-N
- References
- (1) V.V. Ganusov et al.: J. Immun. Meth. 298(1-2), 183 (2005) , (2) C. Bergsdorf et al.: FEBS Letters 536(1-3), 120 (2003) , (3) I.E. Dumitriu et al.: Analyt. Biochem. 299, 247 (2001) , (4) A. Lyons et al.: Meth. Cell Biol. 63, 375 (2001)
- InChi
- InChI=1S/C24H16O7.C6H7NO4/c1-13(25)28-15-7-9-19-21(11-15)30-22-12-16(29-14(2)26)8-10-20(22)24(19)18-6-4-3-5-17(18)23(27)31-24
5(6)-Carboxyfluorescein diacetate N-succinimidyl ester is a useful fluorescent tracer that can passivley diffuse into cells and covalently label intracellular proteins, resulting in long-term cell labeling. The reagent itself is colorless and nonfluorescent but becomes brightly green fluorescent once it is hydrolyzed by intracellular esterases.